Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel
CONCLUSIONS: The results demonstrated the better redifferentiation effect of DCs with the combinational use of transgenic TGF-β3 and a microcavitary alginate hydrogel and implied that DCs would be alternative seed cells for cartilage tissue engineering due to their easily achieved sufficient cell amounts through multiple passages and great potential to redifferentiate to produce cartilaginous extracellular matrix.PMID:38523201 | DOI:10.1007/s10529-024-03475-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Yongchang Yao Ke Chen Qian Pan Hui Gao Weixian Su Shicong Zheng Weiqiang Dong Dongyang Qian Source Type: research
Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus
CONCLUSION: Cloning of gene gju768 and rational design based on surface charge engineering contributed to the identification of thermostable esterase from Geobacillus sp. and the exploration of evolutionary strategies for thermal stability.PMID:38523202 | DOI:10.1007/s10529-024-03473-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Runfei Song Jin Zhang Mengyu Zhu Lin Lin Wei Wei Dongzhi Wei Source Type: research
A recombinant fungal photolyase autonomously enters human cell nuclei to fix UV-induced DNA lesions
In this study, we aim to construct recombinant CPD photolyases that can autonomously enter human cell nuclei to fix UV-induced CPDs. A fly cell penetration peptide and a viral nucleus localization signal peptide were recombined with a fungal CPD photolyase to construct a recombinant protein. This engineered CPD photolyase autonomously crosses cytoplasm and nuclear membrane of human cell nuclei, which then efficiently photo-repairs UV-induced CPD lesions in the genomic DNA. This further protects the cells by increasing SOD activity, and decreasing cellular ROSs, malondialdehyde and apoptosis.PMID:38523200 | DOI:10.1007/s105...
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Yuting Bao Weiguo Fang Source Type: research
Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel
CONCLUSIONS: The results demonstrated the better redifferentiation effect of DCs with the combinational use of transgenic TGF-β3 and a microcavitary alginate hydrogel and implied that DCs would be alternative seed cells for cartilage tissue engineering due to their easily achieved sufficient cell amounts through multiple passages and great potential to redifferentiate to produce cartilaginous extracellular matrix.PMID:38523201 | DOI:10.1007/s10529-024-03475-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Yongchang Yao Ke Chen Qian Pan Hui Gao Weixian Su Shicong Zheng Weiqiang Dong Dongyang Qian Source Type: research
Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus
CONCLUSION: Cloning of gene gju768 and rational design based on surface charge engineering contributed to the identification of thermostable esterase from Geobacillus sp. and the exploration of evolutionary strategies for thermal stability.PMID:38523202 | DOI:10.1007/s10529-024-03473-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Runfei Song Jin Zhang Mengyu Zhu Lin Lin Wei Wei Dongzhi Wei Source Type: research
A recombinant fungal photolyase autonomously enters human cell nuclei to fix UV-induced DNA lesions
In this study, we aim to construct recombinant CPD photolyases that can autonomously enter human cell nuclei to fix UV-induced CPDs. A fly cell penetration peptide and a viral nucleus localization signal peptide were recombined with a fungal CPD photolyase to construct a recombinant protein. This engineered CPD photolyase autonomously crosses cytoplasm and nuclear membrane of human cell nuclei, which then efficiently photo-repairs UV-induced CPD lesions in the genomic DNA. This further protects the cells by increasing SOD activity, and decreasing cellular ROSs, malondialdehyde and apoptosis.PMID:38523200 | DOI:10.1007/s105...
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Yuting Bao Weiguo Fang Source Type: research
Redifferentiation of genetically modified dedifferentiated chondrocytes in a microcavitary hydrogel
CONCLUSIONS: The results demonstrated the better redifferentiation effect of DCs with the combinational use of transgenic TGF-β3 and a microcavitary alginate hydrogel and implied that DCs would be alternative seed cells for cartilage tissue engineering due to their easily achieved sufficient cell amounts through multiple passages and great potential to redifferentiate to produce cartilaginous extracellular matrix.PMID:38523201 | DOI:10.1007/s10529-024-03475-2 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Yongchang Yao Ke Chen Qian Pan Hui Gao Weixian Su Shicong Zheng Weiqiang Dong Dongyang Qian Source Type: research
Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus
CONCLUSION: Cloning of gene gju768 and rational design based on surface charge engineering contributed to the identification of thermostable esterase from Geobacillus sp. and the exploration of evolutionary strategies for thermal stability.PMID:38523202 | DOI:10.1007/s10529-024-03473-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 25, 2024 Category: Biotechnology Authors: Runfei Song Jin Zhang Mengyu Zhu Lin Lin Wei Wei Dongzhi Wei Source Type: research
Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica
CONCLUSION: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.PMID:38493279 | DOI:10.1007/s10529-024-03472-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 17, 2024 Category: Biotechnology Authors: Qiang Wilson Yan Ying-Chun Liu Christa Barrett Kelly Haake Daniel Seeler Oliver May Ross Zirkle Source Type: research
Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica
CONCLUSION: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.PMID:38493279 | DOI:10.1007/s10529-024-03472-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 17, 2024 Category: Biotechnology Authors: Qiang Wilson Yan Ying-Chun Liu Christa Barrett Kelly Haake Daniel Seeler Oliver May Ross Zirkle Source Type: research
Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica
CONCLUSION: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.PMID:38493279 | DOI:10.1007/s10529-024-03472-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 17, 2024 Category: Biotechnology Authors: Qiang Wilson Yan Ying-Chun Liu Christa Barrett Kelly Haake Daniel Seeler Oliver May Ross Zirkle Source Type: research
Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica
CONCLUSION: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.PMID:38493279 | DOI:10.1007/s10529-024-03472-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 17, 2024 Category: Biotechnology Authors: Qiang Wilson Yan Ying-Chun Liu Christa Barrett Kelly Haake Daniel Seeler Oliver May Ross Zirkle Source Type: research
Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica
CONCLUSION: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.PMID:38493279 | DOI:10.1007/s10529-024-03472-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 17, 2024 Category: Biotechnology Authors: Qiang Wilson Yan Ying-Chun Liu Christa Barrett Kelly Haake Daniel Seeler Oliver May Ross Zirkle Source Type: research
Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica
CONCLUSION: Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.PMID:38493279 | DOI:10.1007/s10529-024-03472-5 (Source: Biotechnology Letters)
Source: Biotechnology Letters - March 17, 2024 Category: Biotechnology Authors: Qiang Wilson Yan Ying-Chun Liu Christa Barrett Kelly Haake Daniel Seeler Oliver May Ross Zirkle Source Type: research
Recycling selectable markers via Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins
CONCLUSIONS: With easy manipulation, the method was effective in recycling of the selectable markers, and consequently two protein genes were sequential integrated chromosomally and successfully co-expressed in the yeast.PMID:38416308 | DOI:10.1007/s10529-024-03466-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 28, 2024 Category: Biotechnology Authors: Weixian Wang Minghai Han Guofei Zhu Xiaohui Liu Tianming Zhao Xiaoyan Ma Xun Gong Cunbin Xu Source Type: research
