Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology
Biotechnol Lett. 2023 Dec 8. doi: 10.1007/s10529-023-03444-1. Online ahead of print.ABSTRACTMetabolic Engineering of yeast is a critical approach to improving the production capacity of cell factories. To obtain genetically stable recombinant strains, the exogenous DNA is preferred to be integrated into the genome. Previously, we developed a Golden Gate toolkit YALIcloneNHEJ, which could be used as an efficient modular cloning toolkit for the random integration of multigene pathways through the innate non-homologous end-joining repair mechanisms of Yarrowia lipolytica. We expanded the toolkit by designing additional buildi...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Qi Shen Fang Yan Ya-Wen Li Jian Wang Jia Ji Wen-Xin Yan Dan-Chen He Ping Song Tian-Qiong Shi Source Type: research
Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production
CONCLUSIONS: The ADD production of the rough-type M. neoaurum R was notably enhanced by the immobilization onto semi-expanded perlite. Moreover, the ADD batch yields of M. neoaurum R immobilized by semi-expanded perlite were maintained at high levels during the repeated-batch fermentation.PMID:38064040 | DOI:10.1007/s10529-023-03448-x (Source: Biotechnology Letters)
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Anqi Zhao Yamei Li Lixia Wu Zhi Wang Yongkun Lv Wenlong Xiong Mohammad Asraful Alam Guohua Liu Jingliang Xu Source Type: research
Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica
The objective of this study was to obtain sufficient information on the thermal stabilization of a food-grade lipase from Thermomyces lanuginosus (TLL) using the immobilization technique. To do this, a new non-porous support was prepared via the sequential extraction of SiO2 from rice husks, followed by functionalization with (3-aminopropyl) triethoxysilane - 3-APTES (Amino-SiO2), and activation with glutaraldehyde - GA (GA-Amino-SiO2). We evaluated the influence of GA concentration, which varied from 0.25% v v-1 to 4% v v-1, on the immobilization parameters and enzyme thermal stabilization. The thermal inactivation parame...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Iara C A Bolina Adriano A Mendes Source Type: research
Production of D-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae
Biotechnol Lett. 2023 Dec 8. doi: 10.1007/s10529-023-03443-2. Online ahead of print.ABSTRACTD-Glucaric acid is a potential biobased platform chemical. Previously mainly Escherichia coli, but also the yeast Saccharomyces cerevisiae, and Pichia pastoris, have been engineered for conversion of D-glucose to D-glucaric acid via myo-inositol. One reason for low yields from the yeast strains is the strong flux towards glycolysis. Thus, to decrease the flux of D-glucose to biomass, and to increase D-glucaric acid yield, the four step D-glucaric acid pathway was introduced into a phosphoglucose isomerase deficient (Pgi1p-deficient)...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Mervi Toivari Maija-Leena Vehkom äki Laura Ruohonen Merja Penttil ä Marilyn G Wiebe Source Type: research
Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology
Biotechnol Lett. 2023 Dec 8. doi: 10.1007/s10529-023-03444-1. Online ahead of print.ABSTRACTMetabolic Engineering of yeast is a critical approach to improving the production capacity of cell factories. To obtain genetically stable recombinant strains, the exogenous DNA is preferred to be integrated into the genome. Previously, we developed a Golden Gate toolkit YALIcloneNHEJ, which could be used as an efficient modular cloning toolkit for the random integration of multigene pathways through the innate non-homologous end-joining repair mechanisms of Yarrowia lipolytica. We expanded the toolkit by designing additional buildi...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Qi Shen Fang Yan Ya-Wen Li Jian Wang Jia Ji Wen-Xin Yan Dan-Chen He Ping Song Tian-Qiong Shi Source Type: research
Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production
CONCLUSIONS: The ADD production of the rough-type M. neoaurum R was notably enhanced by the immobilization onto semi-expanded perlite. Moreover, the ADD batch yields of M. neoaurum R immobilized by semi-expanded perlite were maintained at high levels during the repeated-batch fermentation.PMID:38064040 | DOI:10.1007/s10529-023-03448-x (Source: Biotechnology Letters)
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Anqi Zhao Yamei Li Lixia Wu Zhi Wang Yongkun Lv Wenlong Xiong Mohammad Asraful Alam Guohua Liu Jingliang Xu Source Type: research
Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica
The objective of this study was to obtain sufficient information on the thermal stabilization of a food-grade lipase from Thermomyces lanuginosus (TLL) using the immobilization technique. To do this, a new non-porous support was prepared via the sequential extraction of SiO2 from rice husks, followed by functionalization with (3-aminopropyl) triethoxysilane - 3-APTES (Amino-SiO2), and activation with glutaraldehyde - GA (GA-Amino-SiO2). We evaluated the influence of GA concentration, which varied from 0.25% v v-1 to 4% v v-1, on the immobilization parameters and enzyme thermal stabilization. The thermal inactivation parame...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Iara C A Bolina Adriano A Mendes Source Type: research
Production of D-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae
Biotechnol Lett. 2023 Dec 8. doi: 10.1007/s10529-023-03443-2. Online ahead of print.ABSTRACTD-Glucaric acid is a potential biobased platform chemical. Previously mainly Escherichia coli, but also the yeast Saccharomyces cerevisiae, and Pichia pastoris, have been engineered for conversion of D-glucose to D-glucaric acid via myo-inositol. One reason for low yields from the yeast strains is the strong flux towards glycolysis. Thus, to decrease the flux of D-glucose to biomass, and to increase D-glucaric acid yield, the four step D-glucaric acid pathway was introduced into a phosphoglucose isomerase deficient (Pgi1p-deficient)...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Mervi Toivari Maija-Leena Vehkom äki Laura Ruohonen Merja Penttil ä Marilyn G Wiebe Source Type: research
Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology
Biotechnol Lett. 2023 Dec 8. doi: 10.1007/s10529-023-03444-1. Online ahead of print.ABSTRACTMetabolic Engineering of yeast is a critical approach to improving the production capacity of cell factories. To obtain genetically stable recombinant strains, the exogenous DNA is preferred to be integrated into the genome. Previously, we developed a Golden Gate toolkit YALIcloneNHEJ, which could be used as an efficient modular cloning toolkit for the random integration of multigene pathways through the innate non-homologous end-joining repair mechanisms of Yarrowia lipolytica. We expanded the toolkit by designing additional buildi...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Qi Shen Fang Yan Ya-Wen Li Jian Wang Jia Ji Wen-Xin Yan Dan-Chen He Ping Song Tian-Qiong Shi Source Type: research
Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production
CONCLUSIONS: The ADD production of the rough-type M. neoaurum R was notably enhanced by the immobilization onto semi-expanded perlite. Moreover, the ADD batch yields of M. neoaurum R immobilized by semi-expanded perlite were maintained at high levels during the repeated-batch fermentation.PMID:38064040 | DOI:10.1007/s10529-023-03448-x (Source: Biotechnology Letters)
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Anqi Zhao Yamei Li Lixia Wu Zhi Wang Yongkun Lv Wenlong Xiong Mohammad Asraful Alam Guohua Liu Jingliang Xu Source Type: research
Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica
The objective of this study was to obtain sufficient information on the thermal stabilization of a food-grade lipase from Thermomyces lanuginosus (TLL) using the immobilization technique. To do this, a new non-porous support was prepared via the sequential extraction of SiO2 from rice husks, followed by functionalization with (3-aminopropyl) triethoxysilane - 3-APTES (Amino-SiO2), and activation with glutaraldehyde - GA (GA-Amino-SiO2). We evaluated the influence of GA concentration, which varied from 0.25% v v-1 to 4% v v-1, on the immobilization parameters and enzyme thermal stabilization. The thermal inactivation parame...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Iara C A Bolina Adriano A Mendes Source Type: research
Production of D-glucaric acid with phosphoglucose isomerase-deficient Saccharomyces cerevisiae
Biotechnol Lett. 2023 Dec 8. doi: 10.1007/s10529-023-03443-2. Online ahead of print.ABSTRACTD-Glucaric acid is a potential biobased platform chemical. Previously mainly Escherichia coli, but also the yeast Saccharomyces cerevisiae, and Pichia pastoris, have been engineered for conversion of D-glucose to D-glucaric acid via myo-inositol. One reason for low yields from the yeast strains is the strong flux towards glycolysis. Thus, to decrease the flux of D-glucose to biomass, and to increase D-glucaric acid yield, the four step D-glucaric acid pathway was introduced into a phosphoglucose isomerase deficient (Pgi1p-deficient)...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Mervi Toivari Maija-Leena Vehkom äki Laura Ruohonen Merja Penttil ä Marilyn G Wiebe Source Type: research
Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology
Biotechnol Lett. 2023 Dec 8. doi: 10.1007/s10529-023-03444-1. Online ahead of print.ABSTRACTMetabolic Engineering of yeast is a critical approach to improving the production capacity of cell factories. To obtain genetically stable recombinant strains, the exogenous DNA is preferred to be integrated into the genome. Previously, we developed a Golden Gate toolkit YALIcloneNHEJ, which could be used as an efficient modular cloning toolkit for the random integration of multigene pathways through the innate non-homologous end-joining repair mechanisms of Yarrowia lipolytica. We expanded the toolkit by designing additional buildi...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Qi Shen Fang Yan Ya-Wen Li Jian Wang Jia Ji Wen-Xin Yan Dan-Chen He Ping Song Tian-Qiong Shi Source Type: research
Immobilization of rough morphotype Mycolicibacterium neoaurum R for androstadienedione production
CONCLUSIONS: The ADD production of the rough-type M. neoaurum R was notably enhanced by the immobilization onto semi-expanded perlite. Moreover, the ADD batch yields of M. neoaurum R immobilized by semi-expanded perlite were maintained at high levels during the repeated-batch fermentation.PMID:38064040 | DOI:10.1007/s10529-023-03448-x (Source: Biotechnology Letters)
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Anqi Zhao Yamei Li Lixia Wu Zhi Wang Yongkun Lv Wenlong Xiong Mohammad Asraful Alam Guohua Liu Jingliang Xu Source Type: research
Kinetic and thermodynamic studies on the thermal inactivation of lipase immobilized on glutaraldehyde-activated rice husk silica
The objective of this study was to obtain sufficient information on the thermal stabilization of a food-grade lipase from Thermomyces lanuginosus (TLL) using the immobilization technique. To do this, a new non-porous support was prepared via the sequential extraction of SiO2 from rice husks, followed by functionalization with (3-aminopropyl) triethoxysilane - 3-APTES (Amino-SiO2), and activation with glutaraldehyde - GA (GA-Amino-SiO2). We evaluated the influence of GA concentration, which varied from 0.25% v v-1 to 4% v v-1, on the immobilization parameters and enzyme thermal stabilization. The thermal inactivation parame...
Source: Biotechnology Letters - December 8, 2023 Category: Biotechnology Authors: Iara C A Bolina Adriano A Mendes Source Type: research
