Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system
CONCLUSION: The production of MSC-EVs in a 3D bioreactor system under hypoxic conditions resulted in increased EV concentration and purity. This system could be especially useful in screening culture conditions for the production of 3D-derived MSC-EVs.PMID:38349512 | DOI:10.1007/s10529-024-03465-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 13, 2024 Category: Biotechnology Authors: Ciarra Almeria Ren é Weiss Maike Keck Viktoria Weber Cornelia Kasper Dominik Egger Source Type: research
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system
CONCLUSION: The production of MSC-EVs in a 3D bioreactor system under hypoxic conditions resulted in increased EV concentration and purity. This system could be especially useful in screening culture conditions for the production of 3D-derived MSC-EVs.PMID:38349512 | DOI:10.1007/s10529-024-03465-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 13, 2024 Category: Biotechnology Authors: Ciarra Almeria Ren é Weiss Maike Keck Viktoria Weber Cornelia Kasper Dominik Egger Source Type: research
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system
CONCLUSION: The production of MSC-EVs in a 3D bioreactor system under hypoxic conditions resulted in increased EV concentration and purity. This system could be especially useful in screening culture conditions for the production of 3D-derived MSC-EVs.PMID:38349512 | DOI:10.1007/s10529-024-03465-4 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 13, 2024 Category: Biotechnology Authors: Ciarra Almeria Ren é Weiss Maike Keck Viktoria Weber Cornelia Kasper Dominik Egger Source Type: research
Effect of magnesium oxide nanoparticles and LED irradiation on the viability and differentiation of human stem cells of the apical papilla
CONCLUSION: This research concluded that exposure to SCAPs, MgONPs, and LED irradiation has a significant effect on enhancing gene expression of odontogenic/osteogenic markers and increasing matrix mineralization.PMID:38326543 | DOI:10.1007/s10529-024-03471-6 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 7, 2024 Category: Biotechnology Authors: Hamed Karkehabadi Afsaneh Rahmati Hadiseh Abbaspourrokni Abbas Farmany Rezvan Najafi Rooholah Behroozi Loghman Rezaei-Soufi Roshanak Abbasi Source Type: research
Production of bilirubin via whole-cell transformation utilizing recombinant Corynebacterium glutamicum expressing a β-glucuronidase from Staphylococcus sp. RLH1
In this study, we employed Corynebacterium glutamicum ATCC13032 to express a β-glucuronidase (StGUS), an enzyme from Staphylococcus sp. RLH1 that effectively hydrolyzes conjugated bilirubin to bilirubin. Following the optimization of the biotransformation conditions, a remarkable conversion rate of 79.7% in the generation of bilirubin was obtained at temperate 40 °C, pH 7.0, 1 mM Mg2+ and 6 mM antioxidant NaHSO3 after 12 h. These findings hold significant potential for establishing an industrially viable platform for large-scale bilirubin production.PMID:38310624 | DOI:10.1007/s10529-024-03468-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 4, 2024 Category: Biotechnology Authors: Wei Zhou Yanan Cui Mengyun Chen Qijun Gao Kai Bao Yongzhong Wang Min Zhang Source Type: research
Performance of a combined electrotrophic and electrogenic biofilm operated under long-term, continuous cycling
CONCLUSIONS: These results indicate that bidirectional electrogenic/electrotrophic biofilms are capable of efficient charge storage/release over a wide range of cycling frequency and may eventually enable development of sustainable, high energy density rechargeable batteries.PMID:38300444 | DOI:10.1007/s10529-023-03450-3 (Source: Biotechnology Letters)
Source: Biotechnology Letters - February 1, 2024 Category: Biotechnology Authors: Matthew D Yates Rebecca L Mickol Amelia Vignola Jeffrey W Baldwin Sarah M Glaven Leonard M Tender Source Type: research
Discovery, structural characterization, and functional insights into a novel apiosidase from the GH140 family, isolated from a lignocellulolytic-enriched mangrove microbial community
CONCLUSION: This enzyme represents a novel tool for researchers interested in studying the physiology and structure of plant cell walls and developing biocatalytic strategies for drug and flavor production.PMID:38280177 | DOI:10.1007/s10529-023-03460-1 (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 27, 2024 Category: Biotechnology Authors: Marcelo Vizona Liberato Douglas Antonio Alvaredo Paixao Geizecler Tomazetto Didier Ndeh David N Bolam Fabio Marcio Squina Source Type: research
Silica nanoparticles enhance the cyto- and hemocompatibility of a multilayered extracellular matrix scaffold for vascular tissue regeneration
CONCLUSION: Our findings suggest that the addition of SiNP to human amniotic membrane extracellular matrixes improves the cyto- and hemocompatibility of rolled scaffolds and highlights this strategy as a robust mechanism to stabilize layered collagen scaffolds for vascular tissue regeneration.PMID:38279044 | DOI:10.1007/s10529-023-03459-8 (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 26, 2024 Category: Biotechnology Authors: Leslie A Goldberg Helena D Zomer Calum McFetridge Peter S McFetridge Source Type: research
An inducible CRISPRi circuit for tunable dynamic regulation of gene expression in Saccharopolyspora erythraea
This study demonstrated the effectiveness of an inducible gene downregulation approach using CRISPRi and a cumate-inducible promoter, providing valuable insights for optimizing natural product production in actinomyces.PMID:38279045 | DOI:10.1007/s10529-023-03462-z (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 26, 2024 Category: Biotechnology Authors: Xing Jiang Xiang Ke Xiwei Tian Ju Chu Source Type: research
Isolation of delignifying bacteria and optimization of microbial pretreatment of biomass for bioenergy
This study focused on the isolation and characterization of two novel delignifying bacteria, GIET1 and GIET2, to enhance cellulose accessibility by lignin degradation. Molecular characterization confirmed their genetic identities, providing valuable microbial resources for biofuel production. Our results revealed distinct preferences for temperature, pH, and incubation period for the two bacteria. Bacillus haynesii exhibited optimal performance under moderate conditions and shorter incubation period, making it suitable for rice straw and sugarcane bagasse pretreatment. In contrast, Paenibacillus alvei thrived at higher tem...
Source: Biotechnology Letters - January 22, 2024 Category: Biotechnology Authors: B Rabi Prasad Suman Polaki Radha Krushna Padhi Source Type: research
Differentiation, maturation, and collection of THP-1-derived dendritic cells based on a PEG hydrogel culture platform
CONCLUSION: The collected evidence indicated that the proteolytically degradable PEG hydrogel matrix promoted DC differentiation and maturation. In addition, the matrix stiffness control could manipulate the marker expressions of differentiation and maturation. Particularly, the mature DC was successfully collected from the hydrogel matrix. These results highlighted the PEG hydrogel-based DC culture might be a useful tool for potential DC-based immunotherapies.PMID:38231384 | DOI:10.1007/s10529-023-03457-w (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 17, 2024 Category: Biotechnology Authors: Jaeho Choi Chang Seok Ki Source Type: research
Retraction Note: Characterization of a new bacteriocin-like inhibitory peptide produced by Lactobacillus sakei B-RKM 0559
Biotechnol Lett. 2024 Jan 18. doi: 10.1007/s10529-024-03470-7. Online ahead of print.NO ABSTRACTPMID:38233693 | DOI:10.1007/s10529-024-03470-7 (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 17, 2024 Category: Biotechnology Authors: Gulyaim K Abitayeva Maira S Urazova Arman S Abilkhadirov Zinigul S Sarmurzina Serik M Shaikhin Source Type: research
Highly efficient biosynthesis of salidroside by a UDP-glucosyltransferase-catalyzed cascade reaction
CONCLUSION: This is the highest salidroside titer ever reported so far using biocatalytic approach.PMID:38184486 | DOI:10.1007/s10529-023-03453-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 6, 2024 Category: Biotechnology Authors: Guosi Li Qilin Xu Nan Hu Xinyang Liu Yiqi Jiang Hailong Xue Yongjun Zang Fucheng Zhu Source Type: research
Construction of a bacteriophage-derived vector with potential applications in targeted drug delivery and cell imaging
Biotechnol Lett. 2024 Jan 6. doi: 10.1007/s10529-023-03455-y. Online ahead of print.ABSTRACTThere is a strong relationship between the dysregulation of epidermal growth factor receptor (EGFR) and the development of epithelial-derived cancers. Therefore, EGFR has usually been considered the desired target for gene therapy. Here, we propose an approach for targeting EGFR-expressing cells by phage particles capable of displaying EGF and GFP as tumor-targeting and reporting elements, respectively. For this purpose, the superfolder GFP-EGF (sfGFP-EGF) coding sequence was inserted at the N-terminus of the pIII gene in the pIT2 p...
Source: Biotechnology Letters - January 6, 2024 Category: Biotechnology Authors: Mehdi Sharifi Ali Akbar Alizadeh Maryam Hamzeh Mivehroud Siavoush Dastmalchi Source Type: research
Highly efficient biosynthesis of salidroside by a UDP-glucosyltransferase-catalyzed cascade reaction
CONCLUSION: This is the highest salidroside titer ever reported so far using biocatalytic approach.PMID:38184486 | DOI:10.1007/s10529-023-03453-0 (Source: Biotechnology Letters)
Source: Biotechnology Letters - January 6, 2024 Category: Biotechnology Authors: Guosi Li Qilin Xu Nan Hu Xinyang Liu Yiqi Jiang Hailong Xue Yongjun Zang Fucheng Zhu Source Type: research
