A recombinant fungal photolyase autonomously enters human cell nuclei to fix UV-induced DNA lesions

In this study, we aim to construct recombinant CPD photolyases that can autonomously enter human cell nuclei to fix UV-induced CPDs. A fly cell penetration peptide and a viral nucleus localization signal peptide were recombined with a fungal CPD photolyase to construct a recombinant protein. This engineered CPD photolyase autonomously crosses cytoplasm and nuclear membrane of human cell nuclei, which then efficiently photo-repairs UV-induced CPD lesions in the genomic DNA. This further protects the cells by increasing SOD activity, and decreasing cellular ROSs, malondialdehyde and apoptosis.PMID:38523200 | DOI:10.1007/s10529-024-03474-3
Source: Biotechnology Letters - Category: Biotechnology Authors: Source Type: research