3D-QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti-hyperplasia drugs
Biotechnol J. 2024 Feb;19(2):e2300437. doi: 10.1002/biot.202300437.ABSTRACTPsoriasis is a common immune-mediated skin condition characterized by aberrant keratinocytes and cell proliferation. The purpose of this study was to explore the FDA-approved drugs by 3D-QSAR pharmacophore model and evaluate their efficiency by in-silico, in vitro, and in vivo psoriasis animal model. A 3D-QSAR pharmacophore model was developed by utilizing HypoGen algorithm using the structural features of 48 diaryl derivatives with diverse molecular patterns. The model was validated by a test set of 27 compounds, by cost analysis method, and Fische...
Source: Biotechnology Journal - February 25, 2024 Category: Biotechnology Authors: Muhammad Zafar Irshad Khan Dildar Khan Muhammad Yasir Akbar Hao Wang Ihsan-Ul Haq Jian-Zhong Chen Source Type: research
CFD evaluation of hydrophobic feedstock bench-scale fermenters for efficient high agitation volumetric mass transfer
Biotechnol J. 2024 Feb;19(2):e2300384. doi: 10.1002/biot.202300384.ABSTRACTA new biomanufacturing platform combining intracellular metabolic engineering of the oleaginous yeast Yarrowia lipolytica and extracellular bioreaction engineering provides efficient bioconversion of plant oils/animal fats into high-value products. However, predicting the hydrodynamics and mass transfer parameters is difficult due to the high agitation and sparging required to create dispersed oil droplets in an aqueous medium for efficient yeast fermentation. In the current study, commercial computational fluid dynamic (CFD) solver Ansys CFX couple...
Source: Biotechnology Journal - February 25, 2024 Category: Biotechnology Authors: Richard Marx Huolong Liu Seongkyu Yoon Dongming Xie Source Type: research
CRISPR/Cas system: A revolutionary tool for crop improvement
Biotechnol J. 2024 Feb;19(2):e2300298. doi: 10.1002/biot.202300298.ABSTRACTWorld's population is elevating at an alarming rate thus, the rising demands of producing crops with better adaptability to biotic and abiotic stresses, superior nutritional as well as morphological qualities, and generation of high-yielding varieties have led to encourage the development of new plant breeding technologies. The availability and easy accessibility of genome sequences for a number of crop plants as well as the development of various genome editing technologies such as zinc finger nucleases (ZFNs), transcription activator-like effector...
Source: Biotechnology Journal - February 25, 2024 Category: Biotechnology Authors: Ayushi Mishra Veda P Pandey Source Type: research
Innovative translational platforms for rapid developing clinical vaccines against COVID-19 and other infectious disease
Biotechnol J. 2024 Feb;19(2):e2300658. doi: 10.1002/biot.202300658.ABSTRACTA vaccine is a biological preparation that contains the antigen capable of stimulating the immune system to form the defense against pathogens. Vaccine development often confronts big challenges, including time/energy-consuming, low efficacy, lag to pathogen emergence and mutation, and even safety concern. However, these seem now mostly conquerable through constructing the advanced translational platforms that can make innovative vaccines, sometimes, potentiated with a distinct multifunctional VADS (vaccine adjuvant delivery system), as evidenced by...
Source: Biotechnology Journal - February 25, 2024 Category: Biotechnology Authors: Ning Wang Ting Wang Source Type: research
Effect of extracellular matrices on production and potency of mesenchymal stem cell-derived exosomes
Biotechnol J. 2024 Feb;19(2):e2300474. doi: 10.1002/biot.202300474.ABSTRACTMesenchymal stem cell (MSC) derived exosomes have emerged as potential acellular therapeutics for various tissue regenerative applications. However, successful clinical translation of exosome-based therapy is limited by lack of a structured production platform. Thus, in this study, the effect of decellularized extracellular matrix (dECM) was assessed on the production and potency of exosomes secreted by bone marrow-derived human MSCs. The results indicate that there was a ∼2-fold increase in MSC-exosome production when MSCs were cultured on dECM c...
Source: Biotechnology Journal - February 25, 2024 Category: Biotechnology Authors: Rittika Somadder Lina Faraj Saurav Datta Mathumai Kanapathipillai Gargi Ghosh Source Type: research
Recombinant production of a highly efficient photolyase from Thermus thermophilus
Biotechnol J. 2024 Jan;19(2):e2300325. doi: 10.1002/biot.202300325.ABSTRACTUltraviolet (UV) radiation from sunlight can damage DNA, inducing mutagenesis and eventually leading to skin cancer. Topical sunscreens are used to avoid the effect of UV irradiation, but the topical application of DNA repair enzymes, such as photolyase, can provide active photoprotection by DNA recovery. Here we produced a recombinant Thermus thermophilus photolyase expressed in Escherichia coli, evaluated the kinetic parameters of bacterial growth and the kinetics and stability of the enzyme. The maximum biomass (𝑋𝑚𝑎𝑥 ) of 2.0 g L-1 wa...
Source: Biotechnology Journal - February 22, 2024 Category: Biotechnology Authors: Karin Torres-Obreque Felipe Gobbi Gon çalves Rafael Bertelli Ferraro Fabiana Fuentes-Le ón Carlos Frederico Martins Menck Tales Alexandre Costa-Silva Gisele Monteiro Patrizia Perego Carlota de Oliveira Rangel-Yagui Source Type: research
Explainable deep learning enhances robust and reliable real-time monitoring of a chromatographic protein A capture step
This study represents a major step toward improved viability of data-driven models in biopharmaceutical manufacturing.PMID:38385524 | DOI:10.1002/biot.202300554 (Source: Biotechnology Journal)
Source: Biotechnology Journal - February 22, 2024 Category: Biotechnology Authors: Matthias Medl Friedrich Leisch Astrid D ürauer Theresa Scharl Source Type: research
Recombinant production of a highly efficient photolyase from Thermus thermophilus
Biotechnol J. 2024 Jan;19(2):e2300325. doi: 10.1002/biot.202300325.ABSTRACTUltraviolet (UV) radiation from sunlight can damage DNA, inducing mutagenesis and eventually leading to skin cancer. Topical sunscreens are used to avoid the effect of UV irradiation, but the topical application of DNA repair enzymes, such as photolyase, can provide active photoprotection by DNA recovery. Here we produced a recombinant Thermus thermophilus photolyase expressed in Escherichia coli, evaluated the kinetic parameters of bacterial growth and the kinetics and stability of the enzyme. The maximum biomass (𝑋𝑚𝑎𝑥 ) of 2.0 g L-1 wa...
Source: Biotechnology Journal - February 22, 2024 Category: Biotechnology Authors: Karin Torres-Obreque Felipe Gobbi Gon çalves Rafael Bertelli Ferraro Fabiana Fuentes-Le ón Carlos Frederico Martins Menck Tales Alexandre Costa-Silva Gisele Monteiro Patrizia Perego Carlota de Oliveira Rangel-Yagui Source Type: research
Explainable deep learning enhances robust and reliable real-time monitoring of a chromatographic protein A capture step
This study represents a major step toward improved viability of data-driven models in biopharmaceutical manufacturing.PMID:38385524 | DOI:10.1002/biot.202300554 (Source: Biotechnology Journal)
Source: Biotechnology Journal - February 22, 2024 Category: Biotechnology Authors: Matthias Medl Friedrich Leisch Astrid D ürauer Theresa Scharl Source Type: research
Recombinant production of a highly efficient photolyase from Thermus thermophilus
Biotechnol J. 2024 Jan;19(2):e2300325. doi: 10.1002/biot.202300325.ABSTRACTUltraviolet (UV) radiation from sunlight can damage DNA, inducing mutagenesis and eventually leading to skin cancer. Topical sunscreens are used to avoid the effect of UV irradiation, but the topical application of DNA repair enzymes, such as photolyase, can provide active photoprotection by DNA recovery. Here we produced a recombinant Thermus thermophilus photolyase expressed in Escherichia coli, evaluated the kinetic parameters of bacterial growth and the kinetics and stability of the enzyme. The maximum biomass (𝑋𝑚𝑎𝑥 ) of 2.0 g L-1 wa...
Source: Biotechnology Journal - February 22, 2024 Category: Biotechnology Authors: Karin Torres-Obreque Felipe Gobbi Gon çalves Rafael Bertelli Ferraro Fabiana Fuentes-Le ón Carlos Frederico Martins Menck Tales Alexandre Costa-Silva Gisele Monteiro Patrizia Perego Carlota de Oliveira Rangel-Yagui Source Type: research
Explainable deep learning enhances robust and reliable real-time monitoring of a chromatographic protein A capture step
This study represents a major step toward improved viability of data-driven models in biopharmaceutical manufacturing.PMID:38385524 | DOI:10.1002/biot.202300554 (Source: Biotechnology Journal)
Source: Biotechnology Journal - February 22, 2024 Category: Biotechnology Authors: Matthias Medl Friedrich Leisch Astrid D ürauer Theresa Scharl Source Type: research
Production of < sub > D < /sub > -tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess
This study provides a promising sustainable process for functional food (D -tagatose) production. Moreover, this process fully utilized whey powder, demonstrating good atom economy.PMID:38375553 | DOI:10.1002/biot.202300415 (Source: Biotechnology Journal)
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Wei Ma Fengyi Li Longyue Li Bin Li Kangle Niu Qinghua Liu Laichuang Han Lijuan Han Xu Fang Source Type: research
Dual cytoplasmic-peroxisomal compartmentalization engineering and multiple metabolic engineering strategies for high yield non-psychoactive cannabinoid in Saccharomyces cerevisiae
In this study, we developed an engineered cell factory for CBG production using Saccharomyces cerevisiae. We introduced the CBGA biosynthetic pathway into S. cerevisiae and employed several strategies to enhance CBGA production. These strategies included dynamically inhibiting the competitive bypass of key metabolic pathways regulated by Erg20p. Additionally, we implemented a dual cytoplasmic-peroxisomal compartmentalization approach to further increase CBGA production. Furthermore, we ensured efficient CBGA production by optimizing NADPH and acetyl-CoA pools. Ultimately, our engineered strain achieved a CBG titer of 138 m...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yun-Kun Ding Yuan Ning Di Xin Yu-Jie Fu Source Type: research
A high-fidelity, dual site-specific integration system in CHO cells by a Bxb1 recombinase
Biotechnol J. 2024 Jan;19(2):e2300410. doi: 10.1002/biot.202300410.ABSTRACTSite-specific integration (SSI) via recombinase mediated cassette exchange (RMCE) has shown advantages over random integration methods for expression of biotherapeutics. As an extension of our previous work developing SSI host cells, we developed a dual-site SSI system having two independent integration sites at different genomic loci, each containing a unique landing pad (LP). This system was leveraged to generate and compare two RMCE hosts, one (dFRT) compatible with the Flp recombinase, the other (dBxb1) compatible with the Bxb1 recombinase. Our ...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yifeng Xu Kerstin B Crowe Paulena L Lieske Michael Barnes Kalpanie Bandara Jianlin Chu Wei Wei John J Scarcelli Lin Zhang Source Type: research
Establishment and application of quantitative detection of Bacillus velezensis HMB26553, a biocontrol agent against cotton damping-off caused by Rhizoctonia
Biotechnol J. 2024 Jan;19(2):e2300412. doi: 10.1002/biot.202300412.ABSTRACTA highly sensitive quantitative PCR (qPCR) method was developed for detection and quantification of Bacillus velezensis HMB26553 in cotton rhizosphere. The study aimed to develop a quantitative detection method for the strain HMB26553, and explore the relationship between its colonization of the cotton rhizosphere and its control effect. The whole genome sequence of strain HMB26553 was obtained by genome sequencing and a unique specific sequence pB-gene0026 on plasmid plaBV2 was identified by using high-throughput alignment against NCBI. Plasmid pla...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Zhenhe Su Gaoge Liu Cong Li Xiaomeng Liu Qinggang Guo Peipei Wang Lihong Dong Xiuyun Lu Weisong Zhao Xiaoyun Zhang Yuanghang Qu Jiaqi Zhang Shaojing Mo Shezeng Li Ping Ma Source Type: research
