Construction and characterization of a functional variant hFGF7 with enhanced properties by circular permutation
Biotechnol J. 2024 Mar;19(3):e2300712. doi: 10.1002/biot.202300712.ABSTRACTHuman fibroblast growth factor 7 (hFGF7) is a member of the paracrine-acting FGF family and mediates various reactions such as wound healing, tissue homeostasis, and liver regeneration. These activities make it a plausible candidate for pharmaceutical applications as a drug. However, the low expression level and stability of the recombinant hFGF7 were known to be major hurdles for further applications. Here, the expression level and stability of hFGF7 were attempted to improve by changing the order of amino acids through circular permutation (CP), t...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Hye-Ji Choi Hanui Lee Dae-Eun Cheong Su-Kyoung Yoo Dong-Eun Lee Geun-Joong Kim Source Type: research
Development of synthetic small regulatory RNA for Rhodococcus erythropolis
In this study, synthetic sRNA strategy was exploited for gene repression in R. erythropolis XP. The synthetic sRNA based on the RhlS scaffold from Pseudomonas aeruginosa functions better in repressing sfgfp expression than those based on E. coli MicC, SgrS, and P. aeruginosa PrrF1-2 scaffold. The RhlS-based sRNAs were applied to study the influence of sulfur metabolism on biodesulfurization (BDS) efficiency in R. erythropolis XP and successfully identified two genes involved in sulfur metabolism that affect the BDS efficiency significantly. The RhlS-based synthetic sRNAs show promise in the metabolic engineering of Rhodoco...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Lijuan Wang Jie Hou Kun Yang Haonan Yu Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Characterization of cellular responses and cell lysis to elevated hydrodynamic stress from benchtop perfusion bioreactors
Biotechnol J. 2024 Mar;19(3):e2400063. doi: 10.1002/biot.202400063.ABSTRACTThe effective design of perfusion cell culture is currently challenging regarding balancing the operating parameters associated with the hydrodynamic conditions due to increased system complexity. To address this issue, cellular responses of an industrial CHO cell line to different types of hydrodynamic stress in benchtop perfusion bioreactors originating from agitation, sparging, and hollow fibers (HF) in the cell retention devices were systematically investigated here with the analysis of cell lysis. It was found that cell lysis was very common an...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Weijian Zhang Qingyuan Ran Liang Zhao Qian Ye Wen-Song Tan Source Type: research
Fc engineered anti-virus therapeutic human IgG < sub > 1 < /sub > expressed in plants with altered binding to the neonatal Fc receptor
In this study, Fc-engineering of antirabies virus therapeutic mAb SO57 with the endoplasmic reticulum (ER)-retention peptide signal (Lys-Asp-Glu-Leu; KDEL) (mAbpK SO57) in plant cell was conducted to enhance its binding activity to human neonatal Fc receptor (hFcRn), consequently improve its serum half-life. Enzyme-linked immunosorbent assay (ELISA) and Surface plasmon resonance assay showed altered binding affinity of the Fc region of three different mAbpK SO57 variants [M252Y/S254T/T256E (MST), M428L/N434S (MN), H433K/N434F (HN)] to hFcRn compared to wild type (WT) of mAbpK SO57. Molecular modeling data visualized the st...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Sol-Ah Park Yoonji Lee Hyunjoo Hwang Jeong Hwan Lee Yang Joo Kang Yerin Kim Caiquan Jin Hyun Joo An Yoo Jin Oh Peter Hinterdorfer Eunhye Kim Sun Choi Kisung Ko Source Type: research
An innovative hybrid modeling approach for simultaneous prediction of cell culture process dynamics and product quality
Biotechnol J. 2024 Mar;19(3):e2300473. doi: 10.1002/biot.202300473.ABSTRACTThe use of hybrid models is extensively described in the literature to predict the process evolution in cell cultures. These models combine mechanistic and machine learning methods, allowing the prediction of complex process behavior, in the presence of many process variables, without the need to collect a large amount of data. Hybrid models cannot be directly used to predict final product critical quality attributes, or CQAs, because they are usually measured only at the end of the process, and more mechanistic knowledge is needed for many classes ...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Jakub Polak Zhuangrong Huang Michael Sokolov Moritz von Stosch Alessandro Butt é C Eric Hodgman Michael Borys Anurag Khetan Source Type: research
R2D ligase: Unveiling a novel DNA ligase with surprising DNA-to-RNA ligation activity
Biotechnol J. 2024 Mar;19(3):e2300711. doi: 10.1002/biot.202300711.ABSTRACTDNA ligases catalyze bond formation in the backbone of nucleic acids via the formation of a phosphodiester bond between adjacent 5' phosphates and 3' hydroxyl groups on one strand of the duplex. While DNA ligases preferentially ligate single breaks in double-stranded DNA (dsDNA), they are capable of ligating a multitude of other nucleic acid substrates like blunt-ended dsDNA, TA overhangs, short overhangs and various DNA-RNA hybrids. Here we report a novel DNA ligase from Cronobacter phage CR 9 (R2D Ligase) with an unexpected DNA-to-RNA ligation act...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Sigurd Eidem Gundes ø Ulli Rothweiler Elise Heimland Yvonne Piotrowski Inger Kristine R ødum Jenny Johansson S öderberg Ildik ó Moustaka Gábor Terese Solstad Adele Williamson Olav Lanes Bernd Ketelsen Striberny Source Type: research
Construction and characterization of a functional variant hFGF7 with enhanced properties by circular permutation
Biotechnol J. 2024 Mar;19(3):e2300712. doi: 10.1002/biot.202300712.ABSTRACTHuman fibroblast growth factor 7 (hFGF7) is a member of the paracrine-acting FGF family and mediates various reactions such as wound healing, tissue homeostasis, and liver regeneration. These activities make it a plausible candidate for pharmaceutical applications as a drug. However, the low expression level and stability of the recombinant hFGF7 were known to be major hurdles for further applications. Here, the expression level and stability of hFGF7 were attempted to improve by changing the order of amino acids through circular permutation (CP), t...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Hye-Ji Choi Hanui Lee Dae-Eun Cheong Su-Kyoung Yoo Dong-Eun Lee Geun-Joong Kim Source Type: research
Development of synthetic small regulatory RNA for Rhodococcus erythropolis
In this study, synthetic sRNA strategy was exploited for gene repression in R. erythropolis XP. The synthetic sRNA based on the RhlS scaffold from Pseudomonas aeruginosa functions better in repressing sfgfp expression than those based on E. coli MicC, SgrS, and P. aeruginosa PrrF1-2 scaffold. The RhlS-based sRNAs were applied to study the influence of sulfur metabolism on biodesulfurization (BDS) efficiency in R. erythropolis XP and successfully identified two genes involved in sulfur metabolism that affect the BDS efficiency significantly. The RhlS-based synthetic sRNAs show promise in the metabolic engineering of Rhodoco...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Lijuan Wang Jie Hou Kun Yang Haonan Yu Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Characterization of cellular responses and cell lysis to elevated hydrodynamic stress from benchtop perfusion bioreactors
Biotechnol J. 2024 Mar;19(3):e2400063. doi: 10.1002/biot.202400063.ABSTRACTThe effective design of perfusion cell culture is currently challenging regarding balancing the operating parameters associated with the hydrodynamic conditions due to increased system complexity. To address this issue, cellular responses of an industrial CHO cell line to different types of hydrodynamic stress in benchtop perfusion bioreactors originating from agitation, sparging, and hollow fibers (HF) in the cell retention devices were systematically investigated here with the analysis of cell lysis. It was found that cell lysis was very common an...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Weijian Zhang Qingyuan Ran Liang Zhao Qian Ye Wen-Song Tan Source Type: research
Fc engineered anti-virus therapeutic human IgG < sub > 1 < /sub > expressed in plants with altered binding to the neonatal Fc receptor
In this study, Fc-engineering of antirabies virus therapeutic mAb SO57 with the endoplasmic reticulum (ER)-retention peptide signal (Lys-Asp-Glu-Leu; KDEL) (mAbpK SO57) in plant cell was conducted to enhance its binding activity to human neonatal Fc receptor (hFcRn), consequently improve its serum half-life. Enzyme-linked immunosorbent assay (ELISA) and Surface plasmon resonance assay showed altered binding affinity of the Fc region of three different mAbpK SO57 variants [M252Y/S254T/T256E (MST), M428L/N434S (MN), H433K/N434F (HN)] to hFcRn compared to wild type (WT) of mAbpK SO57. Molecular modeling data visualized the st...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Sol-Ah Park Yoonji Lee Hyunjoo Hwang Jeong Hwan Lee Yang Joo Kang Yerin Kim Caiquan Jin Hyun Joo An Yoo Jin Oh Peter Hinterdorfer Eunhye Kim Sun Choi Kisung Ko Source Type: research
An innovative hybrid modeling approach for simultaneous prediction of cell culture process dynamics and product quality
Biotechnol J. 2024 Mar;19(3):e2300473. doi: 10.1002/biot.202300473.ABSTRACTThe use of hybrid models is extensively described in the literature to predict the process evolution in cell cultures. These models combine mechanistic and machine learning methods, allowing the prediction of complex process behavior, in the presence of many process variables, without the need to collect a large amount of data. Hybrid models cannot be directly used to predict final product critical quality attributes, or CQAs, because they are usually measured only at the end of the process, and more mechanistic knowledge is needed for many classes ...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Jakub Polak Zhuangrong Huang Michael Sokolov Moritz von Stosch Alessandro Butt é C Eric Hodgman Michael Borys Anurag Khetan Source Type: research
R2D ligase: Unveiling a novel DNA ligase with surprising DNA-to-RNA ligation activity
Biotechnol J. 2024 Mar;19(3):e2300711. doi: 10.1002/biot.202300711.ABSTRACTDNA ligases catalyze bond formation in the backbone of nucleic acids via the formation of a phosphodiester bond between adjacent 5' phosphates and 3' hydroxyl groups on one strand of the duplex. While DNA ligases preferentially ligate single breaks in double-stranded DNA (dsDNA), they are capable of ligating a multitude of other nucleic acid substrates like blunt-ended dsDNA, TA overhangs, short overhangs and various DNA-RNA hybrids. Here we report a novel DNA ligase from Cronobacter phage CR 9 (R2D Ligase) with an unexpected DNA-to-RNA ligation act...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Sigurd Eidem Gundes ø Ulli Rothweiler Elise Heimland Yvonne Piotrowski Inger Kristine R ødum Jenny Johansson S öderberg Ildik ó Moustaka Gábor Terese Solstad Adele Williamson Olav Lanes Bernd Ketelsen Striberny Source Type: research
Construction and characterization of a functional variant hFGF7 with enhanced properties by circular permutation
Biotechnol J. 2024 Mar;19(3):e2300712. doi: 10.1002/biot.202300712.ABSTRACTHuman fibroblast growth factor 7 (hFGF7) is a member of the paracrine-acting FGF family and mediates various reactions such as wound healing, tissue homeostasis, and liver regeneration. These activities make it a plausible candidate for pharmaceutical applications as a drug. However, the low expression level and stability of the recombinant hFGF7 were known to be major hurdles for further applications. Here, the expression level and stability of hFGF7 were attempted to improve by changing the order of amino acids through circular permutation (CP), t...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Hye-Ji Choi Hanui Lee Dae-Eun Cheong Su-Kyoung Yoo Dong-Eun Lee Geun-Joong Kim Source Type: research
Development of synthetic small regulatory RNA for Rhodococcus erythropolis
In this study, synthetic sRNA strategy was exploited for gene repression in R. erythropolis XP. The synthetic sRNA based on the RhlS scaffold from Pseudomonas aeruginosa functions better in repressing sfgfp expression than those based on E. coli MicC, SgrS, and P. aeruginosa PrrF1-2 scaffold. The RhlS-based sRNAs were applied to study the influence of sulfur metabolism on biodesulfurization (BDS) efficiency in R. erythropolis XP and successfully identified two genes involved in sulfur metabolism that affect the BDS efficiency significantly. The RhlS-based synthetic sRNAs show promise in the metabolic engineering of Rhodoco...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Lijuan Wang Jie Hou Kun Yang Haonan Yu Bo Zhang Zhiqiang Liu Yuguo Zheng Source Type: research
Characterization of cellular responses and cell lysis to elevated hydrodynamic stress from benchtop perfusion bioreactors
Biotechnol J. 2024 Mar;19(3):e2400063. doi: 10.1002/biot.202400063.ABSTRACTThe effective design of perfusion cell culture is currently challenging regarding balancing the operating parameters associated with the hydrodynamic conditions due to increased system complexity. To address this issue, cellular responses of an industrial CHO cell line to different types of hydrodynamic stress in benchtop perfusion bioreactors originating from agitation, sparging, and hollow fibers (HF) in the cell retention devices were systematically investigated here with the analysis of cell lysis. It was found that cell lysis was very common an...
Source: Biotechnology Journal - March 26, 2024 Category: Biotechnology Authors: Weijian Zhang Qingyuan Ran Liang Zhao Qian Ye Wen-Song Tan Source Type: research
