Production of < sub > D < /sub > -tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess
This study provides a promising sustainable process for functional food (D -tagatose) production. Moreover, this process fully utilized whey powder, demonstrating good atom economy.PMID:38375553 | DOI:10.1002/biot.202300415 (Source: Biotechnology Journal)
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Wei Ma Fengyi Li Longyue Li Bin Li Kangle Niu Qinghua Liu Laichuang Han Lijuan Han Xu Fang Source Type: research
Dual cytoplasmic-peroxisomal compartmentalization engineering and multiple metabolic engineering strategies for high yield non-psychoactive cannabinoid in Saccharomyces cerevisiae
In this study, we developed an engineered cell factory for CBG production using Saccharomyces cerevisiae. We introduced the CBGA biosynthetic pathway into S. cerevisiae and employed several strategies to enhance CBGA production. These strategies included dynamically inhibiting the competitive bypass of key metabolic pathways regulated by Erg20p. Additionally, we implemented a dual cytoplasmic-peroxisomal compartmentalization approach to further increase CBGA production. Furthermore, we ensured efficient CBGA production by optimizing NADPH and acetyl-CoA pools. Ultimately, our engineered strain achieved a CBG titer of 138 m...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yun-Kun Ding Yuan Ning Di Xin Yu-Jie Fu Source Type: research
A high-fidelity, dual site-specific integration system in CHO cells by a Bxb1 recombinase
Biotechnol J. 2024 Jan;19(2):e2300410. doi: 10.1002/biot.202300410.ABSTRACTSite-specific integration (SSI) via recombinase mediated cassette exchange (RMCE) has shown advantages over random integration methods for expression of biotherapeutics. As an extension of our previous work developing SSI host cells, we developed a dual-site SSI system having two independent integration sites at different genomic loci, each containing a unique landing pad (LP). This system was leveraged to generate and compare two RMCE hosts, one (dFRT) compatible with the Flp recombinase, the other (dBxb1) compatible with the Bxb1 recombinase. Our ...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yifeng Xu Kerstin B Crowe Paulena L Lieske Michael Barnes Kalpanie Bandara Jianlin Chu Wei Wei John J Scarcelli Lin Zhang Source Type: research
Establishment and application of quantitative detection of Bacillus velezensis HMB26553, a biocontrol agent against cotton damping-off caused by Rhizoctonia
Biotechnol J. 2024 Jan;19(2):e2300412. doi: 10.1002/biot.202300412.ABSTRACTA highly sensitive quantitative PCR (qPCR) method was developed for detection and quantification of Bacillus velezensis HMB26553 in cotton rhizosphere. The study aimed to develop a quantitative detection method for the strain HMB26553, and explore the relationship between its colonization of the cotton rhizosphere and its control effect. The whole genome sequence of strain HMB26553 was obtained by genome sequencing and a unique specific sequence pB-gene0026 on plasmid plaBV2 was identified by using high-throughput alignment against NCBI. Plasmid pla...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Zhenhe Su Gaoge Liu Cong Li Xiaomeng Liu Qinggang Guo Peipei Wang Lihong Dong Xiuyun Lu Weisong Zhao Xiaoyun Zhang Yuanghang Qu Jiaqi Zhang Shaojing Mo Shezeng Li Ping Ma Source Type: research
Amino acid degradation pathway inhibitory by-products trigger apoptosis in CHO cells
Biotechnol J. 2024 Jan;19(2):e2300338. doi: 10.1002/biot.202300338.ABSTRACTChinese hamster ovary (CHO) cells are widely used to produce complex biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand for such products. One way to enhance productivity is by cultivating cells at high densities, but inhibitory by-products, such as metabolite derivatives from amino acid degradation, can hinder achieving high cell densities. This research examines the impact of these inhibitory by-products on high-density cultures. We cultured X1 and X2 CHO cell lines in a small-scale semi-perfusion system an...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Ver ónica S Martínez Karen Rodriguez Timothy McCubbin Junjie Tong Stephen Mahler Evan Shave Kym Baker Trent P Munro Esteban Marcellin Source Type: research
A generic pump-free organ-on-a-chip platform for assessment of intestinal drug absorption
In this study, we have developed a generic, pump-free organ-on-a-chip platform consisting of a 32-unit chip and an adjustable rocker, facilitating high-throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump-based systems. Through constructing a gut-on-a-chip model, we observed remarkable enhancements in the expression of barrier-associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis un...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yaqiong Guo Yingying Xie Jianhua Qin Source Type: research
Transforming native exosomes to engineered drug vehicles: A smart solution to modern cancer theranostics
Biotechnol J. 2024 Jan;19(2):e2300370. doi: 10.1002/biot.202300370.ABSTRACTExosomes have been the hidden treasure of the cell in terms of cellular interactions, transportation and therapy. The native exosomes (NEx) secreted by the parent cells hold promising aspects in cancer diagnosis and therapy. NEx has low immunogenicity, high biocompatibility, low toxicity and high stability which enables them to be an ideal prognostic biomarker in cancer diagnosis. However, due to heterogeneity, NEx lacks specificity and accuracy to be used as therapeutic drug delivery vehicle in cancer therapy. Transforming these NEx with their inna...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Rajeev Kumar Sahoo Surya Kant Tripathi Stuti Biswal Munmun Panda Santosh S Mathapati Bijesh Kumar Biswal Source Type: research
Production of < sub > D < /sub > -tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess
This study provides a promising sustainable process for functional food (D -tagatose) production. Moreover, this process fully utilized whey powder, demonstrating good atom economy.PMID:38375553 | DOI:10.1002/biot.202300415 (Source: Biotechnology Journal)
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Wei Ma Fengyi Li Longyue Li Bin Li Kangle Niu Qinghua Liu Laichuang Han Lijuan Han Xu Fang Source Type: research
Dual cytoplasmic-peroxisomal compartmentalization engineering and multiple metabolic engineering strategies for high yield non-psychoactive cannabinoid in Saccharomyces cerevisiae
In this study, we developed an engineered cell factory for CBG production using Saccharomyces cerevisiae. We introduced the CBGA biosynthetic pathway into S. cerevisiae and employed several strategies to enhance CBGA production. These strategies included dynamically inhibiting the competitive bypass of key metabolic pathways regulated by Erg20p. Additionally, we implemented a dual cytoplasmic-peroxisomal compartmentalization approach to further increase CBGA production. Furthermore, we ensured efficient CBGA production by optimizing NADPH and acetyl-CoA pools. Ultimately, our engineered strain achieved a CBG titer of 138 m...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yun-Kun Ding Yuan Ning Di Xin Yu-Jie Fu Source Type: research
A high-fidelity, dual site-specific integration system in CHO cells by a Bxb1 recombinase
Biotechnol J. 2024 Jan;19(2):e2300410. doi: 10.1002/biot.202300410.ABSTRACTSite-specific integration (SSI) via recombinase mediated cassette exchange (RMCE) has shown advantages over random integration methods for expression of biotherapeutics. As an extension of our previous work developing SSI host cells, we developed a dual-site SSI system having two independent integration sites at different genomic loci, each containing a unique landing pad (LP). This system was leveraged to generate and compare two RMCE hosts, one (dFRT) compatible with the Flp recombinase, the other (dBxb1) compatible with the Bxb1 recombinase. Our ...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yifeng Xu Kerstin B Crowe Paulena L Lieske Michael Barnes Kalpanie Bandara Jianlin Chu Wei Wei John J Scarcelli Lin Zhang Source Type: research
Establishment and application of quantitative detection of Bacillus velezensis HMB26553, a biocontrol agent against cotton damping-off caused by Rhizoctonia
Biotechnol J. 2024 Jan;19(2):e2300412. doi: 10.1002/biot.202300412.ABSTRACTA highly sensitive quantitative PCR (qPCR) method was developed for detection and quantification of Bacillus velezensis HMB26553 in cotton rhizosphere. The study aimed to develop a quantitative detection method for the strain HMB26553, and explore the relationship between its colonization of the cotton rhizosphere and its control effect. The whole genome sequence of strain HMB26553 was obtained by genome sequencing and a unique specific sequence pB-gene0026 on plasmid plaBV2 was identified by using high-throughput alignment against NCBI. Plasmid pla...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Zhenhe Su Gaoge Liu Cong Li Xiaomeng Liu Qinggang Guo Peipei Wang Lihong Dong Xiuyun Lu Weisong Zhao Xiaoyun Zhang Yuanghang Qu Jiaqi Zhang Shaojing Mo Shezeng Li Ping Ma Source Type: research
Amino acid degradation pathway inhibitory by-products trigger apoptosis in CHO cells
Biotechnol J. 2024 Jan;19(2):e2300338. doi: 10.1002/biot.202300338.ABSTRACTChinese hamster ovary (CHO) cells are widely used to produce complex biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand for such products. One way to enhance productivity is by cultivating cells at high densities, but inhibitory by-products, such as metabolite derivatives from amino acid degradation, can hinder achieving high cell densities. This research examines the impact of these inhibitory by-products on high-density cultures. We cultured X1 and X2 CHO cell lines in a small-scale semi-perfusion system an...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Ver ónica S Martínez Karen Rodriguez Timothy McCubbin Junjie Tong Stephen Mahler Evan Shave Kym Baker Trent P Munro Esteban Marcellin Source Type: research
A generic pump-free organ-on-a-chip platform for assessment of intestinal drug absorption
In this study, we have developed a generic, pump-free organ-on-a-chip platform consisting of a 32-unit chip and an adjustable rocker, facilitating high-throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump-based systems. Through constructing a gut-on-a-chip model, we observed remarkable enhancements in the expression of barrier-associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis un...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Yaqiong Guo Yingying Xie Jianhua Qin Source Type: research
Transforming native exosomes to engineered drug vehicles: A smart solution to modern cancer theranostics
Biotechnol J. 2024 Jan;19(2):e2300370. doi: 10.1002/biot.202300370.ABSTRACTExosomes have been the hidden treasure of the cell in terms of cellular interactions, transportation and therapy. The native exosomes (NEx) secreted by the parent cells hold promising aspects in cancer diagnosis and therapy. NEx has low immunogenicity, high biocompatibility, low toxicity and high stability which enables them to be an ideal prognostic biomarker in cancer diagnosis. However, due to heterogeneity, NEx lacks specificity and accuracy to be used as therapeutic drug delivery vehicle in cancer therapy. Transforming these NEx with their inna...
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Rajeev Kumar Sahoo Surya Kant Tripathi Stuti Biswal Munmun Panda Santosh S Mathapati Bijesh Kumar Biswal Source Type: research
Production of < sub > D < /sub > -tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess
This study provides a promising sustainable process for functional food (D -tagatose) production. Moreover, this process fully utilized whey powder, demonstrating good atom economy.PMID:38375553 | DOI:10.1002/biot.202300415 (Source: Biotechnology Journal)
Source: Biotechnology Journal - February 20, 2024 Category: Biotechnology Authors: Wei Ma Fengyi Li Longyue Li Bin Li Kangle Niu Qinghua Liu Laichuang Han Lijuan Han Xu Fang Source Type: research
