Evaluation of in vitro corrosion behavior and biocompatibility of poly[xylitol-(1,12-dodecanedioate)](PXDD)-HA coated porous iron for bone scaffolds applications
Biotechnol J. 2024 Mar;19(3):e2300464. doi: 10.1002/biot.202300464.ABSTRACTThe present study evaluates the corrosion behavior of poly[xylitol-(1,12-dodecanedioate)](PXDD)-HA coated porous iron (PXDD140/HA-Fe) and its cell-material interaction aimed for temporary bone scaffold applications. The physicochemical analyses show that the addition of 20 wt.% HA into the PXDD polymers leads to a higher crystallinity and lower surface roughness. The corrosion assessments of the PXDD140/HA-Fe evaluated by electrochemical methods and surface chemistry analysis indicate that HA decelerates Fe corrosion due to a lower hydrolysis rate f...
Source: Biotechnology Journal - March 21, 2024 Category: Biotechnology Authors: Abdul Hakim Md Yusop Wan Fahmin Faiz Wan Ali Farah Hidayah Jamaludin Fatihhi Szali Januddi Murni Nazira Sarian Norazalina Saad Tuck-Whye Wong Arif Hidayat Hadi Nur Source Type: research
Highly chromophoric fluorescent-labeled methionyl-initiator tRNAs applicable in living cells
We present an optimized strategy to effectively generate highly fluorescent initiator-tRNA complexes in living cells. Our strategy allows the generation of Fluo-Met-tRNAiMet complexes. These complexes can have highly chromogenic N-terminal labeling. For generating such complexes, we use either purified fluorescent methionine (PFM) or non-purified fluorescently labeled methionine (NPFM). Furthermore, PFM promotes the active generation of endogenous tRNAi in cells, leading to highly efficient Fluo-Met-tRNAiMet complexes. Finally, PFM-tRNAiMet complexes also facilitate the visualization of native fluorescently labeled Tat bin...
Source: Biotechnology Journal - March 17, 2024 Category: Biotechnology Authors: Jung Min Kim Jinho Jung Source Type: research
Highly chromophoric fluorescent-labeled methionyl-initiator tRNAs applicable in living cells
We present an optimized strategy to effectively generate highly fluorescent initiator-tRNA complexes in living cells. Our strategy allows the generation of Fluo-Met-tRNAiMet complexes. These complexes can have highly chromogenic N-terminal labeling. For generating such complexes, we use either purified fluorescent methionine (PFM) or non-purified fluorescently labeled methionine (NPFM). Furthermore, PFM promotes the active generation of endogenous tRNAi in cells, leading to highly efficient Fluo-Met-tRNAiMet complexes. Finally, PFM-tRNAiMet complexes also facilitate the visualization of native fluorescently labeled Tat bin...
Source: Biotechnology Journal - March 17, 2024 Category: Biotechnology Authors: Jung Min Kim Jinho Jung Source Type: research
Highly chromophoric fluorescent-labeled methionyl-initiator tRNAs applicable in living cells
We present an optimized strategy to effectively generate highly fluorescent initiator-tRNA complexes in living cells. Our strategy allows the generation of Fluo-Met-tRNAiMet complexes. These complexes can have highly chromogenic N-terminal labeling. For generating such complexes, we use either purified fluorescent methionine (PFM) or non-purified fluorescently labeled methionine (NPFM). Furthermore, PFM promotes the active generation of endogenous tRNAi in cells, leading to highly efficient Fluo-Met-tRNAiMet complexes. Finally, PFM-tRNAiMet complexes also facilitate the visualization of native fluorescently labeled Tat bin...
Source: Biotechnology Journal - March 17, 2024 Category: Biotechnology Authors: Jung Min Kim Jinho Jung Source Type: research
Modification of nitrilase based on computer screening and efficient biosynthesis of 4-cyanobenzoic acid
Biotechnol J. 2024 Mar;19(3):e2300706. doi: 10.1002/biot.202300706.ABSTRACT4-cyanobenzoic acid serves as a crucial intermediate for the synthesis of various high-value organic compounds. The enzymatic hydrolysis of terephthalonitrile to produce 4-cyanobenzoic acid using nitrilase offers the advantages of a simple reaction pathway, environmental friendliness, and easy product separation. In order to efficiently develop nitrilases that meet industrial production requirements, the virtual screening method used in the study is established and mature. From a total of 371 amino acids in the nitrilase AfNIT, which exhibits activi...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Yi-Zhong Jin Ji-Dong Shen Yu-Xin Wang Hong-Ming Shao Hai-Jian Xia Jiao-Hua Lin Zhi-Qiang Liu Yu-Guo Zheng Source Type: research
Promoter screening and identification for metabolic regulation in Acremonium chrysogenum
In this study, based on the analysis of the transcriptome profile, 27 candidate promoters were selected to drive the expression of the reporter genes. The promoter activities of this library ranged from 0.0075 to 101 times of the control promoter PAngpdA . Simultaneously, a rapid screening method for potential bidirectional promoters was developed and 4 strong bidirectional promoters from 27 candidate options were identified and validated. Finally, the Golden Gate method was employed to combine promoter modules from the library with various target genes. Through a mixed transformation and screening process, high-yielding s...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Ling Liu Runge Li Xinrun Zhang Zhen Chen Ali Mohsin Haifeng Hang Xiwei Tian Ju Chu Source Type: research
Enhancing the production of adeno-associated virus (AAV)2 and AAV9 with high full capsid ratio in HEK293 cells through design-of-experiment optimization of triple plasmid ratio
Biotechnol J. 2024 Mar;19(3):e2300667. doi: 10.1002/biot.202300667.ABSTRACTThe recombinant adeno-associated virus (rAAV) vectors used in gene therapy are usually produced by transfecting three different plasmids (Adenoviral helper plasmid (pHelper), AAV rep/cap plasmids (pRepCap), and Transgene plasmid (pAAV-GOI)) into human embryonic kidney 293 (HEK293) cells. However, the high proportion of unwanted empty capsids generated during rAAV production is problematic. To simultaneously enhance the genome titer and full capsid ratio, the ratio of the three plasmids transfected into HEK293 cells was optimized using design-of-expe...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Sungje Park Seunghyeon Shin Haeshin Lee Jae-Hyung Jang Gyun Min Lee Source Type: research
Tuning capsid formation dynamics in recombinant adeno-associated virus producing synthetic cell lines to enhance full particle productivity
Biotechnol J. 2024 Mar;19(3):e2400051. doi: 10.1002/biot.202400051.ABSTRACTRecombinant adeno-associated virus (rAAV) is widely used as an in vivo delivery vector for gene therapy. It is used in a very large dose, and the large quantities required for broad applications present manufacturing challenges. We have developed a synthetic biology platform of constructing cell lines integrated with essential viral genes which can be induced to produce rAAV without plasmid transfection or virus transduction. Through iterative design-construct-characterization cycles, we have showcased the potential of this synthetic cell production...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Min Lu Yu-Chieh Lin Han-Jung Kuo Wen Cai Qian Ye Liang Zhao Wei-Shou Hu Source Type: research
Recent advances and fundamentals of microalgae cultivation technology
Biotechnol J. 2024 Mar;19(3):e2300725. doi: 10.1002/biot.202300725.ABSTRACTMicroalgae are considered to be a promising group of organisms for fuel production, waste processing, pharmaceutical applications, and as a source of food components. Unicellular algae are worth being considered because of their capacity to produce comparatively large amounts of lipids, proteins, and vitamins while requiring little room for growth. They can also grow on waste and fix CO2 and nitrogen compounds. However, production costs limit the industrial use of microalgae to the most profitable applications including micronutrient production and ...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Julian M Rozenberg Boris A Sorokin Amina N Mukhambetova Anna A Emelianova Valentin V Kuzmin Oxana Y Belogurova-Ovchinnikova Denis V Kuzmin Source Type: research
Enhanced synthesis of S-adenosyl-L-methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae
Biotechnol J. 2024 Mar;19(3):e2300650. doi: 10.1002/biot.202300650.ABSTRACTS-Adenosyl-L-methionine (SAM) is a substrate for many enzyme-catalyzed reactions and provides methyl groups in numerous biological methylations, and thus has vast applications in the agriculture and medical field. Saccharomyces cerevisiae has been engineered as a platform with significant potential for producing SAM, but the current production has room for improvement. Thus, a method that consists of a series of metabolic engineering strategies was established in this study. These strategies included enhancing SAM synthesis, increasing ATP supply, d...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Wenhan Xiao Xiangliu Shi Haowei Huang Xiaogang Wang Wenshu Liang Jianguo Xu Fei Liu Xiaomei Zhang Guoqiang Xu Jinsong Shi Zhenghong Xu Source Type: research
Enhanced targeting: Production of filamentous bacteriophage monoclonal antibodies using the major coat protein pVIII as anchor
This study not only provides a new idea for simplifying the preparation of antifilamentous bacteriophage antibodies which could apply in filamentous bacteriophage display, but it also presents a novel strategy for preparing antibodies against protein-specific epitopes with high sensitivity.PMID:38479991 | DOI:10.1002/biot.202300688 (Source: Biotechnology Journal)
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Qing Li Jianyu Zhu Ting Yuan Leina Dou Xuezhi Yu Jianzhong Shen Huijuan Yang Zhanhui Wang Source Type: research
Standardized approach for accurate and reliable model development of ion-exchange chromatography based on parameter-by-parameter method and consideration of extra-column effects
Biotechnol J. 2024 Mar;19(3):e2300687. doi: 10.1002/biot.202300687.ABSTRACTDeveloping an accurate and reliable model for chromatographic separation that meets regulatory requirements and ensures consistency in model development remains challenging. In order to address this challenge, a standardized approach was proposed in this study with ion-exchange chromatography (IEC). The approach includes the following steps: liquid flow identification, system and column-specific parameters determination and validation, multi-component system identification, protein amount validation, steric mass action parameters determination and e...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Yu-Cheng Chen Hui-Li Lu Rong-Zhu Wang Guo Sun Xue-Qin Zhang Jing-Qi Liang Alois Jungbauer Shan-Jing Yao Dong-Qiang Lin Source Type: research
Rigid linker peptides improve the stability and anti-inflammation effect of human serum albumin and α-melanocyte-stimulating hormone fusion proteins
In this study, optimization of the structures of fusion proteins was attempted by changing the linker peptide between HSA and α-MSH. The optimization resulted in the improvement of various important characteristics, especially the stability and anti-inflammatory bioactivity, which are important features in protein medicines. Compared to the original linker peptide L0, the 5-amino-acid rigid linker peptide L6 (PAPAP) is the best option for further investigation due to its higher expression (increased by 6.27%), improved purification recovery (increased by 60.8%), excellent thermal stability (Tm = 83.5°C) and better inhibi...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Yiyao Liu Yang Li Xueyan Wei Inam Ullah Shahab Uddin Jiatao Wang Runjie Xia MeiZhu Wang Hui Yang Hongyu Li Source Type: research
Predicting protein retention in ion-exchange chromatography using an open source QSPR workflow
Biotechnol J. 2024 Mar;19(3):e2300708. doi: 10.1002/biot.202300708.ABSTRACTProtein-based biopharmaceuticals require high purity before final formulation to ensure product safety, making process development time consuming. Implementation of computational approaches at the initial stages of process development offers a significant reduction in development efforts. By preselecting process conditions, experimental screening can be limited to only a subset. One such computational selection approach is the application of Quantitative Structure Property Relationship (QSPR) models that describe the properties exploited during puri...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Tim Neijenhuis Olivier Le Bussy Geoffroy Geldhof Marieke E Klijn Marcel Ottens Source Type: research
Modification of nitrilase based on computer screening and efficient biosynthesis of 4-cyanobenzoic acid
Biotechnol J. 2024 Mar;19(3):e2300706. doi: 10.1002/biot.202300706.ABSTRACT4-cyanobenzoic acid serves as a crucial intermediate for the synthesis of various high-value organic compounds. The enzymatic hydrolysis of terephthalonitrile to produce 4-cyanobenzoic acid using nitrilase offers the advantages of a simple reaction pathway, environmental friendliness, and easy product separation. In order to efficiently develop nitrilases that meet industrial production requirements, the virtual screening method used in the study is established and mature. From a total of 371 amino acids in the nitrilase AfNIT, which exhibits activi...
Source: Biotechnology Journal - March 13, 2024 Category: Biotechnology Authors: Yi-Zhong Jin Ji-Dong Shen Yu-Xin Wang Hong-Ming Shao Hai-Jian Xia Jiao-Hua Lin Zhi-Qiang Liu Yu-Guo Zheng Source Type: research
