Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
