Gaidai-Yakimov reliability method for high-dimensional spatio-temporal biosystems
This study presents novel methodology for pandemic risks assessment for a national health system of interest. The 2019 coronavirus disease (COVID-19) is a contagious disease with certain potential for worldwide spread and potentially significant effects on public health globally. Suggested methodology enables risks assessment of an epidemic, that may happen in the near future at any time, and in any national region of interest. Traditional spatio-temporal reliability methodologies do not have benefit of easily handling health system's high-dimensionality and complex cross-correlations between regional observations. Contrar...
Source: Biosystems - November 15, 2023 Category: Biotechnology Authors: Oleg Gaidai Vladimir Yakimov Yuhao Niu Zirui Liu Source Type: research
Gaidai-Yakimov reliability method for high-dimensional spatio-temporal biosystems
This study presents novel methodology for pandemic risks assessment for a national health system of interest. The 2019 coronavirus disease (COVID-19) is a contagious disease with certain potential for worldwide spread and potentially significant effects on public health globally. Suggested methodology enables risks assessment of an epidemic, that may happen in the near future at any time, and in any national region of interest. Traditional spatio-temporal reliability methodologies do not have benefit of easily handling health system's high-dimensionality and complex cross-correlations between regional observations. Contrar...
Source: Biosystems - November 15, 2023 Category: Biotechnology Authors: Oleg Gaidai Vladimir Yakimov Yuhao Niu Zirui Liu Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
Modeling tumor growth using fractal calculus: Insights into tumor dynamics
Biosystems. 2023 Nov 7:105071. doi: 10.1016/j.biosystems.2023.105071. Online ahead of print.ABSTRACTImportant concepts like fractal calculus and fractal analysis, the sum of squared residuals, and Aikaike's information criterion must be thoroughly understood in order to correctly fit cancer-related data using the proposed models. The fractal growth models employed in this work are classified in three main categories: Sigmodial growth models (Logistic, Gompertz, and Richards models), Power Law growth model, and Exponential growth models (Exponential and Exponential-Lineal models)". We fitted the data, computed the sum of sq...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Amirreza Khalili Golmankhaneh S ümeyye Tunç Agnieszka Matylda Schlichtinger Dachel Martinez Asanza Alireza Khalili Golmankhaneh Source Type: research
Overview of the fourth special issue in Code Biology
Biosystems. 2023 Nov 7:105074. doi: 10.1016/j.biosystems.2023.105074. Online ahead of print.NO ABSTRACTPMID:37944633 | DOI:10.1016/j.biosystems.2023.105074 (Source: Biosystems)
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Marcello Barbieri Source Type: research
Unexpected extra exon skipping in the DYSF gene during restoring the reading frame by CRISPR/Cas9
In this study, we evaluated the possibility of permanent skipping of exons 3-4, and 26-27 which deletion does not violate the reading frame and allows to eliminate truncated variants within exons. Editing was performed with simultaneous transfection of two sgRNA- and sa/spCas9-containing plasmids on HEK293T cell cultures and healthy donor myoblasts. Skipping of exons 3-4 was performed by destroying the splicing acceptor sites, and exons 26-27 by cuts in the flanking exons with the corresponding deletion in the DNA. Some unexpected results were obtained, when exons 26-27 were skipped, exon 30 was also absent in the transcri...
Source: Biosystems - November 9, 2023 Category: Biotechnology Authors: Olga Levchenko Irina Panchuk Konstantin Kochergin-Nikitsky Irina Petrova Sabina Nagieva Maxim Pilkin Ivan Yakovlev Svetlana Smirnikhina Roman Deev Alexander Lavrov Source Type: research
