Improved Biorepository to Support Sickle Cell Disease Genomics and Clinical Research: A Practical Approach to Link Patient Data and Biospecimens from Muhimbili Sickle Cell Program, Tanzania
This article documents the physical verification process of biospecimens in the biorepository, connecting them to patient clinical and demographic data and aiding in the planning of future genomic and clinical research studies' experience from the Muhimbili Sickle Cell Program in Dar es Salaam, Tanzania. The biospecimen database was updated with the current biospecimen position following the physical verification and then mapping this information to its demographic and clinical data using demographic identifiers. The biorepository stored 74,079 biospecimens in three -80°C freezers, including 63,345 from 5159 patients enro...
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Upendo Masamu Raphael Z Sangeda Josephine Mgaya Siana Nkya Beatrice Octavian Frank R Mtiiye Joyce Nduguru Agnes Jonathan Daniel Kandonga Irene K Minja Paschal Rugajo Emmanuel Balandya Julie Makani Source Type: research
Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel
Conclusions: Potassium levels increase when serum is refrigerated in collection tubes containing separating gel. This can be attributed to contamination of the serum layer by blood cell components beyond the separating gel.PMID:37943629 | DOI:10.1089/bio.2022.0217 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Kohsuke Yoshida Hiromi Tani Sae Nobuhara Shinji Yamamoto Source Type: research
A Method to Freeze Skin Samples for Cryobanks: A Test of Some Cryoprotectants for an Endangered Deer
This study's objective was to test cryopreservation protocols of skin fragments of M. jucunda, using different cryoprotectants in slow freezing. We evaluated four treatments, composed of three cryoprotectants, dimethyl sulfoxide (DMSO), polyvinylpyrrolidone (PVP), and ethylene glycol (EG), used alone and in combination. There was also a control group where the tissue did not undergo cryopreservation. Skin fragments were collected from the medial region of the pelvic limbs of three individuals. Each fragment was divided into 10 equal parts, standardized by weight, making two pieces for each treatment and control from each a...
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Laura Campos Cassavia Cintra de Oliveira Gabrielle Queiroz Vacari Jos é Maurício Barbanti Duarte Source Type: research
Homology Identification and Cross-Contamination Analysis: A Method for Evaluating the Quality of Biological Samples Stored in a Biobank Using the Advanta Sample ID Genotyping Panel
In this study, we used the Advanta Sample ID genotyping panel to detect homology identification and cross-contamination. We compared the single-nucleotide polymorphism (SNP) typing results of two different samples and calculated the similarity score of homologous sample pairs and nonhomologous sample pairs. Through analysis, we obtained a similarity score cutoff point of 0.8620, which was an effective way to distinguish homology and nonhomology. Cross-contamination was detected in two sets of mixtures (STD8:STD6 and jj3:1-P) mixed at a series of special ratios. Sensitivity was dependent on the sample characteristics and mi...
Source: Biopreservation and Biobanking - October 27, 2023 Category: Biomedical Science Authors: Chao Wang Zebin Hu Xiaoyan Zhang Midie Xu Weixiang Shen Lili Du Menghong Sun Hengjun Gao Source Type: research
Biospecimen Qualification in a Clinical Biobank of Urological Diseases
In this study, we evaluated the quality of biobanked specimens by comparing analytical outcomes at 1, 5, and 10 years after collection. Our results demonstrate that currently available assays and tools can be used by biobank laboratories to support objective biospecimen qualification. We have established a workflow to monitor the quality of different types of biospecimens and, in this study, present the results of a qualification exercise applied to fluid samples and their derivatives in the context of urological diseases.PMID:37878356 | DOI:10.1089/bio.2022.0190 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - October 25, 2023 Category: Biomedical Science Authors: Olga Kofanova Sangita Paul Achilleas Pexaras Camille Bellora Ala Petersons Margaux Schmitt Mohamad Baker Berjaoui Yazan Qaoud Miran Kenk Heidi Wagner Neil Fleshner Fay Betsou Source Type: research
