Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
