CellShip: An Ambient Temperature Transport and Short-Term Storage Medium for Mammalian Cell Cultures
In this study, we describe a novel transportation medium for mammalian cells. Five commonly used cell lines, (HEK293, CHO, HepG2, K562, and Jurkat) were successfully shipped and stored for a minimum of 72 hours and up to 96 hours at ambient temperature, after which, cells were recovered into standard culture conditions. Viability (%) and cell numbers, were examined, before, following the transport/storage period and following the recovery period. In all experiments, cell numbers returned to pretransport/storage concentration within 24-48 hours recovery. Imaging data indicated that HepG2 cells were fully adherent and had es...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Emma Buick Andrew Mead Abeer Alhubaysh Patricia Bou Assi Parijat Das James Dayus Mark Turner Lukasz Kowalski Jenny Murray Derek Renshaw Sebastien Farnaud Source Type: research

The Fifth Edition of the ISBER Best Practices: User Feedback Supports Broad Applicability
Biopreserv Biobank. 2023 Dec;21(6):635-636. doi: 10.1089/bio.2023.29129.adw.NO ABSTRACTPMID:38149934 | DOI:10.1089/bio.2023.29129.adw (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Annemieke De Wilde Source Type: research

Best Practices Make Perfect
Biopreserv Biobank. 2023 Dec;21(6):533-534. doi: 10.1089/bio.2023.29128.editorial.NO ABSTRACTPMID:38149935 | DOI:10.1089/bio.2023.29128.editorial (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Alison Parry-Jones Clare M Allocca Source Type: research

Updating International Society for Biological and Environmental Repositories Best Practices, Fifth Edition: A New Process for Relevance in an Evolving Landscape
Biopreserv Biobank. 2023 Dec;21(6):537-546. doi: 10.1089/bio.2023.0140.ABSTRACTThe recently published fifth edition of the International Society for Biological and Environmental Repositories (ISBER) Best Practices signifies a pivotal milestone in navigating the complexities of repository management. Repositories operate within a constantly evolving landscape influenced by the changing fields of biospecimen science, technology, legal requirements, and ethical considerations. This dynamic is further amplified by unprecedented local and global challenges, such as pandemics, conflicts, and supply chain disruptions. Creating th...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Emma Snapes Jonas J Astrin Nina Bertheussen Kr üger Gregory H Grossman Ellen Hendrickson Nicola Miller Cathy Seiler Source Type: research

The Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoa
In this study, the effects of ferulic acid (0.1, 1, ve 10 mM), tryptophan (5, 25, ve 50 mM), and L-glutamine (10, 50, ve 100 mM) at different doses added to 18% raffinose + 3% skimmed milk powder sperm extender on the freezing of mouse spermatozoa in liquid nitrogen were investigated. The combination of 18% raffinose + 3% skimmed milk powder without additives was used as the control group. Frozen spermatozoa were thawed in a 37°C water bath for 30 seconds. After freeze-thawing, motility, dead spermatozoa ratio, plasma membrane integrity, abnormal acrosome ratio, motility endurance (for 4 hours), and cell apoptosis tests w...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: G ökhan Koçak Cengiz Yildiz Source Type: research

CellShip: An Ambient Temperature Transport and Short-Term Storage Medium for Mammalian Cell Cultures
In this study, we describe a novel transportation medium for mammalian cells. Five commonly used cell lines, (HEK293, CHO, HepG2, K562, and Jurkat) were successfully shipped and stored for a minimum of 72 hours and up to 96 hours at ambient temperature, after which, cells were recovered into standard culture conditions. Viability (%) and cell numbers, were examined, before, following the transport/storage period and following the recovery period. In all experiments, cell numbers returned to pretransport/storage concentration within 24-48 hours recovery. Imaging data indicated that HepG2 cells were fully adherent and had es...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Emma Buick Andrew Mead Abeer Alhubaysh Patricia Bou Assi Parijat Das James Dayus Mark Turner Lukasz Kowalski Jenny Murray Derek Renshaw Sebastien Farnaud Source Type: research

The Fifth Edition of the ISBER Best Practices: User Feedback Supports Broad Applicability
Biopreserv Biobank. 2023 Dec;21(6):635-636. doi: 10.1089/bio.2023.29129.adw.NO ABSTRACTPMID:38149934 | DOI:10.1089/bio.2023.29129.adw (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Annemieke De Wilde Source Type: research

Best Practices Make Perfect
Biopreserv Biobank. 2023 Dec;21(6):533-534. doi: 10.1089/bio.2023.29128.editorial.NO ABSTRACTPMID:38149935 | DOI:10.1089/bio.2023.29128.editorial (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Alison Parry-Jones Clare M Allocca Source Type: research

Updating International Society for Biological and Environmental Repositories Best Practices, Fifth Edition: A New Process for Relevance in an Evolving Landscape
Biopreserv Biobank. 2023 Dec;21(6):537-546. doi: 10.1089/bio.2023.0140.ABSTRACTThe recently published fifth edition of the International Society for Biological and Environmental Repositories (ISBER) Best Practices signifies a pivotal milestone in navigating the complexities of repository management. Repositories operate within a constantly evolving landscape influenced by the changing fields of biospecimen science, technology, legal requirements, and ethical considerations. This dynamic is further amplified by unprecedented local and global challenges, such as pandemics, conflicts, and supply chain disruptions. Creating th...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Emma Snapes Jonas J Astrin Nina Bertheussen Kr üger Gregory H Grossman Ellen Hendrickson Nicola Miller Cathy Seiler Source Type: research

The Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoa
In this study, the effects of ferulic acid (0.1, 1, ve 10 mM), tryptophan (5, 25, ve 50 mM), and L-glutamine (10, 50, ve 100 mM) at different doses added to 18% raffinose + 3% skimmed milk powder sperm extender on the freezing of mouse spermatozoa in liquid nitrogen were investigated. The combination of 18% raffinose + 3% skimmed milk powder without additives was used as the control group. Frozen spermatozoa were thawed in a 37°C water bath for 30 seconds. After freeze-thawing, motility, dead spermatozoa ratio, plasma membrane integrity, abnormal acrosome ratio, motility endurance (for 4 hours), and cell apoptosis tests w...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: G ökhan Koçak Cengiz Yildiz Source Type: research

CellShip: An Ambient Temperature Transport and Short-Term Storage Medium for Mammalian Cell Cultures
In this study, we describe a novel transportation medium for mammalian cells. Five commonly used cell lines, (HEK293, CHO, HepG2, K562, and Jurkat) were successfully shipped and stored for a minimum of 72 hours and up to 96 hours at ambient temperature, after which, cells were recovered into standard culture conditions. Viability (%) and cell numbers, were examined, before, following the transport/storage period and following the recovery period. In all experiments, cell numbers returned to pretransport/storage concentration within 24-48 hours recovery. Imaging data indicated that HepG2 cells were fully adherent and had es...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Emma Buick Andrew Mead Abeer Alhubaysh Patricia Bou Assi Parijat Das James Dayus Mark Turner Lukasz Kowalski Jenny Murray Derek Renshaw Sebastien Farnaud Source Type: research

The Fifth Edition of the ISBER Best Practices: User Feedback Supports Broad Applicability
Biopreserv Biobank. 2023 Dec;21(6):635-636. doi: 10.1089/bio.2023.29129.adw.NO ABSTRACTPMID:38149934 | DOI:10.1089/bio.2023.29129.adw (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Annemieke De Wilde Source Type: research

Best Practices Make Perfect
Biopreserv Biobank. 2023 Dec;21(6):533-534. doi: 10.1089/bio.2023.29128.editorial.NO ABSTRACTPMID:38149935 | DOI:10.1089/bio.2023.29128.editorial (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Alison Parry-Jones Clare M Allocca Source Type: research

Updating International Society for Biological and Environmental Repositories Best Practices, Fifth Edition: A New Process for Relevance in an Evolving Landscape
Biopreserv Biobank. 2023 Dec;21(6):537-546. doi: 10.1089/bio.2023.0140.ABSTRACTThe recently published fifth edition of the International Society for Biological and Environmental Repositories (ISBER) Best Practices signifies a pivotal milestone in navigating the complexities of repository management. Repositories operate within a constantly evolving landscape influenced by the changing fields of biospecimen science, technology, legal requirements, and ethical considerations. This dynamic is further amplified by unprecedented local and global challenges, such as pandemics, conflicts, and supply chain disruptions. Creating th...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: Emma Snapes Jonas J Astrin Nina Bertheussen Kr üger Gregory H Grossman Ellen Hendrickson Nicola Miller Cathy Seiler Source Type: research

The Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoa
In this study, the effects of ferulic acid (0.1, 1, ve 10 mM), tryptophan (5, 25, ve 50 mM), and L-glutamine (10, 50, ve 100 mM) at different doses added to 18% raffinose + 3% skimmed milk powder sperm extender on the freezing of mouse spermatozoa in liquid nitrogen were investigated. The combination of 18% raffinose + 3% skimmed milk powder without additives was used as the control group. Frozen spermatozoa were thawed in a 37°C water bath for 30 seconds. After freeze-thawing, motility, dead spermatozoa ratio, plasma membrane integrity, abnormal acrosome ratio, motility endurance (for 4 hours), and cell apoptosis tests w...
Source: Biopreservation and Biobanking - December 27, 2023 Category: Biomedical Science Authors: G ökhan Koçak Cengiz Yildiz Source Type: research