Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
Quantitative Analysis of Ice Crystal Growth During Freezing of Dimethyl Sulfoxide Solutions Under Alternating Current Electric Fields
Biopreserv Biobank. 2023 Nov 22. doi: 10.1089/bio.2023.0035. Online ahead of print.ABSTRACTDuring cryopreservation, the growth of ice crystals can cause mechanical damage to samples, which is one of the important factors limiting the quality of preserved samples. To enhance the preservation quality of biological samples, scholars have tried various engineering methods. Among them, an electric field is an essential factor affecting solution freezing. Dimethyl sulfoxide, as a commonly used cryoprotectant, can cause mechanical damage to cells due to ice crystals even when freezing at the optimal freezing rate. Water is a stro...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Liting Liu Zirui Wang Menghan Wang Gang Zhao Source Type: research
Comparison of Cryopreservation Media for Mesenchymal Stem Cell Spheroids
In this study, we compared the outcomes after subjecting MSC spheroids to a freeze/thaw cycle in three Good Manufacturing Practices-grade cryopreservation media, CryoStor10 (CS10), Stem-Cellbanker (SCB), and Recovery Cell Culture Freezing Media (RFM) or conventional freezing medium (CM) (CM, Dulbecco's modified Eagle's medium containing 20% fetal bovine serum and 10% dimethyl sulfoxide) as a control for 2 months. The endpoints tested were viability, morphology, and expression of stem cell markers and other relevant genes. The results of LIVE/DEAD™ assays and annexin V/propidium iodide staining suggested that viability wa...
Source: Biopreservation and Biobanking - November 27, 2023 Category: Biomedical Science Authors: Jin Ju Park Ok-Hee Lee Jie-Eun Park Jaejin Cho Source Type: research
The Deceased, Public Health, and Research: Proposing Legal Reforms
Biopreserv Biobank. 2023 Nov 9. doi: 10.1089/bio.2023.0026. Online ahead of print.ABSTRACTThere is little guidance concerning biomedical research using tissues from deceased individuals. Unique ethical and legal challenges gained visibility during the coronavirus disease 2019 (COVID-19) pandemic, when important studies using genome sequencing required access to biological materials from deceased individuals. These studies proposed to determine whether specific genomic profiles were associated with important disease outcomes. Such research has previously required consent from next-of-kin or other surrogate decision makers. ...
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Isabel Baltzan Bartha Maria Knoppers Elisheva Tamar Anne Nemetz Jordan Lerner-Ellis Alexander Bernier Karen Devon Source Type: research
Improved Biorepository to Support Sickle Cell Disease Genomics and Clinical Research: A Practical Approach to Link Patient Data and Biospecimens from Muhimbili Sickle Cell Program, Tanzania
This article documents the physical verification process of biospecimens in the biorepository, connecting them to patient clinical and demographic data and aiding in the planning of future genomic and clinical research studies' experience from the Muhimbili Sickle Cell Program in Dar es Salaam, Tanzania. The biospecimen database was updated with the current biospecimen position following the physical verification and then mapping this information to its demographic and clinical data using demographic identifiers. The biorepository stored 74,079 biospecimens in three -80°C freezers, including 63,345 from 5159 patients enro...
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Upendo Masamu Raphael Z Sangeda Josephine Mgaya Siana Nkya Beatrice Octavian Frank R Mtiiye Joyce Nduguru Agnes Jonathan Daniel Kandonga Irene K Minja Paschal Rugajo Emmanuel Balandya Julie Makani Source Type: research
Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel
Conclusions: Potassium levels increase when serum is refrigerated in collection tubes containing separating gel. This can be attributed to contamination of the serum layer by blood cell components beyond the separating gel.PMID:37943629 | DOI:10.1089/bio.2022.0217 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Kohsuke Yoshida Hiromi Tani Sae Nobuhara Shinji Yamamoto Source Type: research
A Method to Freeze Skin Samples for Cryobanks: A Test of Some Cryoprotectants for an Endangered Deer
This study's objective was to test cryopreservation protocols of skin fragments of M. jucunda, using different cryoprotectants in slow freezing. We evaluated four treatments, composed of three cryoprotectants, dimethyl sulfoxide (DMSO), polyvinylpyrrolidone (PVP), and ethylene glycol (EG), used alone and in combination. There was also a control group where the tissue did not undergo cryopreservation. Skin fragments were collected from the medial region of the pelvic limbs of three individuals. Each fragment was divided into 10 equal parts, standardized by weight, making two pieces for each treatment and control from each a...
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Laura Campos Cassavia Cintra de Oliveira Gabrielle Queiroz Vacari Jos é Maurício Barbanti Duarte Source Type: research
The Deceased, Public Health, and Research: Proposing Legal Reforms
Biopreserv Biobank. 2023 Nov 9. doi: 10.1089/bio.2023.0026. Online ahead of print.ABSTRACTThere is little guidance concerning biomedical research using tissues from deceased individuals. Unique ethical and legal challenges gained visibility during the coronavirus disease 2019 (COVID-19) pandemic, when important studies using genome sequencing required access to biological materials from deceased individuals. These studies proposed to determine whether specific genomic profiles were associated with important disease outcomes. Such research has previously required consent from next-of-kin or other surrogate decision makers. ...
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Isabel Baltzan Bartha Maria Knoppers Elisheva Tamar Anne Nemetz Jordan Lerner-Ellis Alexander Bernier Karen Devon Source Type: research
Improved Biorepository to Support Sickle Cell Disease Genomics and Clinical Research: A Practical Approach to Link Patient Data and Biospecimens from Muhimbili Sickle Cell Program, Tanzania
This article documents the physical verification process of biospecimens in the biorepository, connecting them to patient clinical and demographic data and aiding in the planning of future genomic and clinical research studies' experience from the Muhimbili Sickle Cell Program in Dar es Salaam, Tanzania. The biospecimen database was updated with the current biospecimen position following the physical verification and then mapping this information to its demographic and clinical data using demographic identifiers. The biorepository stored 74,079 biospecimens in three -80°C freezers, including 63,345 from 5159 patients enro...
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Upendo Masamu Raphael Z Sangeda Josephine Mgaya Siana Nkya Beatrice Octavian Frank R Mtiiye Joyce Nduguru Agnes Jonathan Daniel Kandonga Irene K Minja Paschal Rugajo Emmanuel Balandya Julie Makani Source Type: research
Increase in Serum Potassium Levels After Refrigerated Storage: A Component of Blood Clot Contaminates the Serum Layer Over the Separator Gel
Conclusions: Potassium levels increase when serum is refrigerated in collection tubes containing separating gel. This can be attributed to contamination of the serum layer by blood cell components beyond the separating gel.PMID:37943629 | DOI:10.1089/bio.2022.0217 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - November 9, 2023 Category: Biomedical Science Authors: Kohsuke Yoshida Hiromi Tani Sae Nobuhara Shinji Yamamoto Source Type: research
