Direct interaction between the transmembrane helices stabilize cytochrome P450 2B4 and cytochrome b5 redox complex
Biophys Chem. 2023 Aug 8;301:107092. doi: 10.1016/j.bpc.2023.107092. Online ahead of print.ABSTRACTThe catalytic activity of cytochrome P450 2B4 (CYP2B4) is moderated by its cognate redox partner cytochrome b5 (Cyt-b5). The endoplasmic reticulum (ER) membrane and intermolecular transmembrane (TM) interaction between CYP2B4 and Cyt-b5 regulate the substrate catalysis and the reaction rate. This emphasizes the significance of elucidating the molecular basis of CYP2B4 and Cyt-b5 complexation in a membrane environment to better understand the enzymatic activity of CYP2B4. Our previous solid-state NMR studies revealed the membr...
Source: Biophysical Chemistry - August 16, 2023 Category: Chemistry Authors: Bikash R Sahoo Ayyalusamy Ramamoorthy Source Type: research
Direct interaction between the transmembrane helices stabilize cytochrome P450 2B4 and cytochrome b5 redox complex
Biophys Chem. 2023 Aug 8;301:107092. doi: 10.1016/j.bpc.2023.107092. Online ahead of print.ABSTRACTThe catalytic activity of cytochrome P450 2B4 (CYP2B4) is moderated by its cognate redox partner cytochrome b5 (Cyt-b5). The endoplasmic reticulum (ER) membrane and intermolecular transmembrane (TM) interaction between CYP2B4 and Cyt-b5 regulate the substrate catalysis and the reaction rate. This emphasizes the significance of elucidating the molecular basis of CYP2B4 and Cyt-b5 complexation in a membrane environment to better understand the enzymatic activity of CYP2B4. Our previous solid-state NMR studies revealed the membr...
Source: Biophysical Chemistry - August 16, 2023 Category: Chemistry Authors: Bikash R Sahoo Ayyalusamy Ramamoorthy Source Type: research
Direct interaction between the transmembrane helices stabilize cytochrome P450 2B4 and cytochrome b5 redox complex
Biophys Chem. 2023 Aug 8;301:107092. doi: 10.1016/j.bpc.2023.107092. Online ahead of print.ABSTRACTThe catalytic activity of cytochrome P450 2B4 (CYP2B4) is moderated by its cognate redox partner cytochrome b5 (Cyt-b5). The endoplasmic reticulum (ER) membrane and intermolecular transmembrane (TM) interaction between CYP2B4 and Cyt-b5 regulate the substrate catalysis and the reaction rate. This emphasizes the significance of elucidating the molecular basis of CYP2B4 and Cyt-b5 complexation in a membrane environment to better understand the enzymatic activity of CYP2B4. Our previous solid-state NMR studies revealed the membr...
Source: Biophysical Chemistry - August 16, 2023 Category: Chemistry Authors: Bikash R Sahoo Ayyalusamy Ramamoorthy Source Type: research
Direct interaction between the transmembrane helices stabilize cytochrome P450 2B4 and cytochrome b5 redox complex
Biophys Chem. 2023 Aug 8;301:107092. doi: 10.1016/j.bpc.2023.107092. Online ahead of print.ABSTRACTThe catalytic activity of cytochrome P450 2B4 (CYP2B4) is moderated by its cognate redox partner cytochrome b5 (Cyt-b5). The endoplasmic reticulum (ER) membrane and intermolecular transmembrane (TM) interaction between CYP2B4 and Cyt-b5 regulate the substrate catalysis and the reaction rate. This emphasizes the significance of elucidating the molecular basis of CYP2B4 and Cyt-b5 complexation in a membrane environment to better understand the enzymatic activity of CYP2B4. Our previous solid-state NMR studies revealed the membr...
Source: Biophysical Chemistry - August 16, 2023 Category: Chemistry Authors: Bikash R Sahoo Ayyalusamy Ramamoorthy Source Type: research
Non-micellar ganglioside GM1 induces an instantaneous conformational change in A β < sub > 42 < /sub > leading to the modulation of the peptide amyloid-fibril pathway
This study unravels the modulatory effect of non-micellar GM1, a glycosphingolipid frequently released from the damaged neuronal membranes, on Aβ42 amyloid fibril formation. Using far-UV circular dichroism experiments, we observed a change in the peptide secondary structure from random-coil to β-turn structures with subsequent generation of predominantly β-sheet-rich species upon interaction with GM1. Thioflavin-T (ThT) fluorescence assays further indicated that GM1 likely interacts with an amyloidogenic Aβ42 intermediate species leading to a possible formation of GM1-modified Aβ42 fibril. Statistically, no significan...
Source: Biophysical Chemistry - August 7, 2023 Category: Chemistry Authors: Manjeet Kumar Magdalena I Ivanova Ayyalusamy Ramamoorthy Source Type: research
