Pomegranate peel, chokeberry leaves and Ironwort extract as novel natural inhibitors of amylin aggregation and cellular toxicity in pancreatic β cells
Biophys Chem. 2023 Oct 31;304:107130. doi: 10.1016/j.bpc.2023.107130. Online ahead of print.ABSTRACTImpeding or reducing human amylin aggregation and/or its toxicity can be key to preventing pancreatic islet amyloidosis and β-cell loss in patients with Type 2 Diabetes Mellitus (T2DM). Here, Punica granatum (pomegranate) peel, Sideritis raeseri (ironwort) and Aronia melanocarpa (chokeberry) leaf extracts, were tested for their novel anti-aggregative and antitoxic properties in human amylin (hIAPP) treated rat pancreatic insulinoma (INS) cells. The protein aggregation (Th-T) assay revealed an inhibitory trend of all three p...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Achanta Rishisree Brayer Mallory Karnaukhova Elena Jankovic Teodora Zduni ć Gordana Šavikin Katarina Jeremic Aleksandar Source Type: research
Thermally stabilized chondroitin sulfate-hemoglobin nanoparticles and their interaction with bioactive compounds
Biophys Chem. 2023 Oct 29;304:107127. doi: 10.1016/j.bpc.2023.107127. Online ahead of print.ABSTRACTThe preparation of nanoparticles (NPs) based on hemoglobin (Hb) with a fully biocompatible methodology is presented. The spontaneous formation of electrostatic complexes of Hb with chondroitin sulfate (CS) at pH 4 in the polysaccharide/protein mass ratio regime where charge neutrality is met leads to spherical nanostructures with monomodal hydrodynamic radii distribution in the range of 50-100 nm. The integrity of the electrostatic complexes is disturbed at pH 7 as the net electric charge of Hb is very low. Treating the NPs ...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Aristeidis Papagiannopoulos Aggeliki Sklapani Nikolaos Spiliopoulos Source Type: research
Osmotic stress studies of G-protein-coupled receptor rhodopsin activation
Biophys Chem. 2023 Sep 26;304:107112. doi: 10.1016/j.bpc.2023.107112. Online ahead of print.ABSTRACTWe summarize and critically review osmotic stress studies of the G-protein-coupled receptor rhodopsin. Although small amounts of structural water are present in these receptors, the effect of bulk water on their function remains uncertain. Studies of the influences of osmotic stress on the GPCR archetype rhodopsin have given insights into the functional role of water in receptor activation. Experimental work has discovered that osmolytes shift the metarhodopsin equilibrium after photoactivation, either to the active or inact...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Andrey V Struts Alexander V Barmasov Steven D E Fried Kushani S K Hewage Suchithranga M D C Perera Michael F Brown Source Type: research
Pomegranate peel, chokeberry leaves and Ironwort extract as novel natural inhibitors of amylin aggregation and cellular toxicity in pancreatic β cells
Biophys Chem. 2023 Oct 31;304:107130. doi: 10.1016/j.bpc.2023.107130. Online ahead of print.ABSTRACTImpeding or reducing human amylin aggregation and/or its toxicity can be key to preventing pancreatic islet amyloidosis and β-cell loss in patients with Type 2 Diabetes Mellitus (T2DM). Here, Punica granatum (pomegranate) peel, Sideritis raeseri (ironwort) and Aronia melanocarpa (chokeberry) leaf extracts, were tested for their novel anti-aggregative and antitoxic properties in human amylin (hIAPP) treated rat pancreatic insulinoma (INS) cells. The protein aggregation (Th-T) assay revealed an inhibitory trend of all three p...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Achanta Rishisree Brayer Mallory Karnaukhova Elena Jankovic Teodora Zduni ć Gordana Šavikin Katarina Jeremic Aleksandar Source Type: research
Thermally stabilized chondroitin sulfate-hemoglobin nanoparticles and their interaction with bioactive compounds
Biophys Chem. 2023 Oct 29;304:107127. doi: 10.1016/j.bpc.2023.107127. Online ahead of print.ABSTRACTThe preparation of nanoparticles (NPs) based on hemoglobin (Hb) with a fully biocompatible methodology is presented. The spontaneous formation of electrostatic complexes of Hb with chondroitin sulfate (CS) at pH 4 in the polysaccharide/protein mass ratio regime where charge neutrality is met leads to spherical nanostructures with monomodal hydrodynamic radii distribution in the range of 50-100 nm. The integrity of the electrostatic complexes is disturbed at pH 7 as the net electric charge of Hb is very low. Treating the NPs ...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Aristeidis Papagiannopoulos Aggeliki Sklapani Nikolaos Spiliopoulos Source Type: research
Osmotic stress studies of G-protein-coupled receptor rhodopsin activation
Biophys Chem. 2023 Sep 26;304:107112. doi: 10.1016/j.bpc.2023.107112. Online ahead of print.ABSTRACTWe summarize and critically review osmotic stress studies of the G-protein-coupled receptor rhodopsin. Although small amounts of structural water are present in these receptors, the effect of bulk water on their function remains uncertain. Studies of the influences of osmotic stress on the GPCR archetype rhodopsin have given insights into the functional role of water in receptor activation. Experimental work has discovered that osmolytes shift the metarhodopsin equilibrium after photoactivation, either to the active or inact...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Andrey V Struts Alexander V Barmasov Steven D E Fried Kushani S K Hewage Suchithranga M D C Perera Michael F Brown Source Type: research
Pomegranate peel, chokeberry leaves and Ironwort extract as novel natural inhibitors of amylin aggregation and cellular toxicity in pancreatic β cells
Biophys Chem. 2023 Oct 31;304:107130. doi: 10.1016/j.bpc.2023.107130. Online ahead of print.ABSTRACTImpeding or reducing human amylin aggregation and/or its toxicity can be key to preventing pancreatic islet amyloidosis and β-cell loss in patients with Type 2 Diabetes Mellitus (T2DM). Here, Punica granatum (pomegranate) peel, Sideritis raeseri (ironwort) and Aronia melanocarpa (chokeberry) leaf extracts, were tested for their novel anti-aggregative and antitoxic properties in human amylin (hIAPP) treated rat pancreatic insulinoma (INS) cells. The protein aggregation (Th-T) assay revealed an inhibitory trend of all three p...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Achanta Rishisree Brayer Mallory Karnaukhova Elena Jankovic Teodora Zduni ć Gordana Šavikin Katarina Jeremic Aleksandar Source Type: research
Thermally stabilized chondroitin sulfate-hemoglobin nanoparticles and their interaction with bioactive compounds
Biophys Chem. 2023 Oct 29;304:107127. doi: 10.1016/j.bpc.2023.107127. Online ahead of print.ABSTRACTThe preparation of nanoparticles (NPs) based on hemoglobin (Hb) with a fully biocompatible methodology is presented. The spontaneous formation of electrostatic complexes of Hb with chondroitin sulfate (CS) at pH 4 in the polysaccharide/protein mass ratio regime where charge neutrality is met leads to spherical nanostructures with monomodal hydrodynamic radii distribution in the range of 50-100 nm. The integrity of the electrostatic complexes is disturbed at pH 7 as the net electric charge of Hb is very low. Treating the NPs ...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Aristeidis Papagiannopoulos Aggeliki Sklapani Nikolaos Spiliopoulos Source Type: research
Osmotic stress studies of G-protein-coupled receptor rhodopsin activation
Biophys Chem. 2023 Sep 26;304:107112. doi: 10.1016/j.bpc.2023.107112. Online ahead of print.ABSTRACTWe summarize and critically review osmotic stress studies of the G-protein-coupled receptor rhodopsin. Although small amounts of structural water are present in these receptors, the effect of bulk water on their function remains uncertain. Studies of the influences of osmotic stress on the GPCR archetype rhodopsin have given insights into the functional role of water in receptor activation. Experimental work has discovered that osmolytes shift the metarhodopsin equilibrium after photoactivation, either to the active or inact...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Andrey V Struts Alexander V Barmasov Steven D E Fried Kushani S K Hewage Suchithranga M D C Perera Michael F Brown Source Type: research
Pomegranate peel, chokeberry leaves and Ironwort extract as novel natural inhibitors of amylin aggregation and cellular toxicity in pancreatic β cells
Biophys Chem. 2023 Oct 31;304:107130. doi: 10.1016/j.bpc.2023.107130. Online ahead of print.ABSTRACTImpeding or reducing human amylin aggregation and/or its toxicity can be key to preventing pancreatic islet amyloidosis and β-cell loss in patients with Type 2 Diabetes Mellitus (T2DM). Here, Punica granatum (pomegranate) peel, Sideritis raeseri (ironwort) and Aronia melanocarpa (chokeberry) leaf extracts, were tested for their novel anti-aggregative and antitoxic properties in human amylin (hIAPP) treated rat pancreatic insulinoma (INS) cells. The protein aggregation (Th-T) assay revealed an inhibitory trend of all three p...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Achanta Rishisree Brayer Mallory Karnaukhova Elena Jankovic Teodora Zduni ć Gordana Šavikin Katarina Jeremic Aleksandar Source Type: research
Thermally stabilized chondroitin sulfate-hemoglobin nanoparticles and their interaction with bioactive compounds
Biophys Chem. 2023 Oct 29;304:107127. doi: 10.1016/j.bpc.2023.107127. Online ahead of print.ABSTRACTThe preparation of nanoparticles (NPs) based on hemoglobin (Hb) with a fully biocompatible methodology is presented. The spontaneous formation of electrostatic complexes of Hb with chondroitin sulfate (CS) at pH 4 in the polysaccharide/protein mass ratio regime where charge neutrality is met leads to spherical nanostructures with monomodal hydrodynamic radii distribution in the range of 50-100 nm. The integrity of the electrostatic complexes is disturbed at pH 7 as the net electric charge of Hb is very low. Treating the NPs ...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Aristeidis Papagiannopoulos Aggeliki Sklapani Nikolaos Spiliopoulos Source Type: research
Osmotic stress studies of G-protein-coupled receptor rhodopsin activation
Biophys Chem. 2023 Sep 26;304:107112. doi: 10.1016/j.bpc.2023.107112. Online ahead of print.ABSTRACTWe summarize and critically review osmotic stress studies of the G-protein-coupled receptor rhodopsin. Although small amounts of structural water are present in these receptors, the effect of bulk water on their function remains uncertain. Studies of the influences of osmotic stress on the GPCR archetype rhodopsin have given insights into the functional role of water in receptor activation. Experimental work has discovered that osmolytes shift the metarhodopsin equilibrium after photoactivation, either to the active or inact...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Andrey V Struts Alexander V Barmasov Steven D E Fried Kushani S K Hewage Suchithranga M D C Perera Michael F Brown Source Type: research
Pomegranate peel, chokeberry leaves and Ironwort extract as novel natural inhibitors of amylin aggregation and cellular toxicity in pancreatic β cells
Biophys Chem. 2023 Oct 31;304:107130. doi: 10.1016/j.bpc.2023.107130. Online ahead of print.ABSTRACTImpeding or reducing human amylin aggregation and/or its toxicity can be key to preventing pancreatic islet amyloidosis and β-cell loss in patients with Type 2 Diabetes Mellitus (T2DM). Here, Punica granatum (pomegranate) peel, Sideritis raeseri (ironwort) and Aronia melanocarpa (chokeberry) leaf extracts, were tested for their novel anti-aggregative and antitoxic properties in human amylin (hIAPP) treated rat pancreatic insulinoma (INS) cells. The protein aggregation (Th-T) assay revealed an inhibitory trend of all three p...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Achanta Rishisree Brayer Mallory Karnaukhova Elena Jankovic Teodora Zduni ć Gordana Šavikin Katarina Jeremic Aleksandar Source Type: research
Thermally stabilized chondroitin sulfate-hemoglobin nanoparticles and their interaction with bioactive compounds
Biophys Chem. 2023 Oct 29;304:107127. doi: 10.1016/j.bpc.2023.107127. Online ahead of print.ABSTRACTThe preparation of nanoparticles (NPs) based on hemoglobin (Hb) with a fully biocompatible methodology is presented. The spontaneous formation of electrostatic complexes of Hb with chondroitin sulfate (CS) at pH 4 in the polysaccharide/protein mass ratio regime where charge neutrality is met leads to spherical nanostructures with monomodal hydrodynamic radii distribution in the range of 50-100 nm. The integrity of the electrostatic complexes is disturbed at pH 7 as the net electric charge of Hb is very low. Treating the NPs ...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Aristeidis Papagiannopoulos Aggeliki Sklapani Nikolaos Spiliopoulos Source Type: research
Osmotic stress studies of G-protein-coupled receptor rhodopsin activation
Biophys Chem. 2023 Sep 26;304:107112. doi: 10.1016/j.bpc.2023.107112. Online ahead of print.ABSTRACTWe summarize and critically review osmotic stress studies of the G-protein-coupled receptor rhodopsin. Although small amounts of structural water are present in these receptors, the effect of bulk water on their function remains uncertain. Studies of the influences of osmotic stress on the GPCR archetype rhodopsin have given insights into the functional role of water in receptor activation. Experimental work has discovered that osmolytes shift the metarhodopsin equilibrium after photoactivation, either to the active or inact...
Source: Biophysical Chemistry - November 12, 2023 Category: Chemistry Authors: Andrey V Struts Alexander V Barmasov Steven D E Fried Kushani S K Hewage Suchithranga M D C Perera Michael F Brown Source Type: research
