Thermodynamics of oligomerization and Helix-to-sheet structural transition of amyloid β-protein on anionic phospholipid vesicles
Biophys Chem. 2024 Apr 18;310:107248. doi: 10.1016/j.bpc.2024.107248. Online ahead of print.ABSTRACTUnderstanding oligomerization and aggregation of the amyloid-β protein is important to elucidate the pathological mechanisms of Alzheimer's disease, and lipid membranes play critical roles in this process. In addition to studies reported by other groups, our group has also reported that the negatively-charged lipid bilayers with a high positive curvature induced α-helix-to-β-sheet conformational transitions of amyloid-β-(1-40) upon increase in protein density on the membrane surface and promoted amyloid fibril formation ...
Source: Biophysical Chemistry - April 23, 2024 Category: Chemistry Authors: Keisuke Ikeda Yuuki Sugiura Hiroyuki Nakao Minoru Nakano Source Type: research
Thermodynamics of oligomerization and Helix-to-sheet structural transition of amyloid β-protein on anionic phospholipid vesicles
Biophys Chem. 2024 Apr 18;310:107248. doi: 10.1016/j.bpc.2024.107248. Online ahead of print.ABSTRACTUnderstanding oligomerization and aggregation of the amyloid-β protein is important to elucidate the pathological mechanisms of Alzheimer's disease, and lipid membranes play critical roles in this process. In addition to studies reported by other groups, our group has also reported that the negatively-charged lipid bilayers with a high positive curvature induced α-helix-to-β-sheet conformational transitions of amyloid-β-(1-40) upon increase in protein density on the membrane surface and promoted amyloid fibril formation ...
Source: Biophysical Chemistry - April 23, 2024 Category: Chemistry Authors: Keisuke Ikeda Yuuki Sugiura Hiroyuki Nakao Minoru Nakano Source Type: research
Insights into the mechanism of peptide fibril growth on gold surface
Biophys Chem. 2024 Apr 15;310:107237. doi: 10.1016/j.bpc.2024.107237. Online ahead of print.ABSTRACTUnderstanding the formation of β-fibrils over the gold surface is of paramount interest in nano-bio-medicinal Chemistry. The intricate mechanism of self-assembly of neurofibrillogenic peptides and their growth over the gold surface remains elusive, as experiments are limited in unveiling the microscopic dynamic details, in particular, at the early stage of the peptide aggregation. In this work, we carried out equilibrium molecular dynamics and enhanced sampling simulations to elucidate the underlying mechanism of the growth...
Source: Biophysical Chemistry - April 19, 2024 Category: Chemistry Authors: Soumya Mondal Tarak Karmakar Source Type: research
Dissecting the effect of ALS mutation S375G on the conformational properties and aggregation dynamics of TDP-43 < sub > 370-375 < /sub > fragment
This study offers for the first time of molecular insights into the S375G mutation affecting the aggregation of TDP-43370-375 at the atomic level, and may open new avenues in the development of future site-specific mutation therapeutics.PMID:38615537 | DOI:10.1016/j.bpc.2024.107230 (Source: Biophysical Chemistry)
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Zhengdong Xu Jianxin Zhang Jiaxing Tang Yehong Gong Yu Zou Qingwen Zhang Source Type: research
Inhibition of insulin fibrillation by carboxyphenylboronic acid-modified chitosan oligosaccharide based on electrostatic interactions and hydrophobic interactions
Biophys Chem. 2024 Apr 8;310:107236. doi: 10.1016/j.bpc.2024.107236. Online ahead of print.ABSTRACTA novel inhibitor, carboxyphenylboronic acid-modified chitosan oligosaccharide (COS-CPBA), was developed by coupling carboxyphenylboronic acid (CPBA) with chitosan oligosaccharide (COS) to inhibit insulin fibrillation. Extensive biophysical assays indicated that COS-CPBA could decelerate insulin aggregation, hinder the conformational transition from α-helix to β-sheet structure, change the morphology of insulin aggregates and alter fibrillation pathway. A mechanism for the inhibition of insulin fibrillation by COS-CPBA was ...
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Xiangyuan Zhao Chunyan Yang Wei Liu Ke Lu Hao Yin Source Type: research
Dissecting the effect of ALS mutation S375G on the conformational properties and aggregation dynamics of TDP-43 < sub > 370-375 < /sub > fragment
This study offers for the first time of molecular insights into the S375G mutation affecting the aggregation of TDP-43370-375 at the atomic level, and may open new avenues in the development of future site-specific mutation therapeutics.PMID:38615537 | DOI:10.1016/j.bpc.2024.107230 (Source: Biophysical Chemistry)
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Zhengdong Xu Jianxin Zhang Jiaxing Tang Yehong Gong Yu Zou Qingwen Zhang Source Type: research
Inhibition of insulin fibrillation by carboxyphenylboronic acid-modified chitosan oligosaccharide based on electrostatic interactions and hydrophobic interactions
Biophys Chem. 2024 Apr 8;310:107236. doi: 10.1016/j.bpc.2024.107236. Online ahead of print.ABSTRACTA novel inhibitor, carboxyphenylboronic acid-modified chitosan oligosaccharide (COS-CPBA), was developed by coupling carboxyphenylboronic acid (CPBA) with chitosan oligosaccharide (COS) to inhibit insulin fibrillation. Extensive biophysical assays indicated that COS-CPBA could decelerate insulin aggregation, hinder the conformational transition from α-helix to β-sheet structure, change the morphology of insulin aggregates and alter fibrillation pathway. A mechanism for the inhibition of insulin fibrillation by COS-CPBA was ...
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Xiangyuan Zhao Chunyan Yang Wei Liu Ke Lu Hao Yin Source Type: research
Dissecting the effect of ALS mutation S375G on the conformational properties and aggregation dynamics of TDP-43 < sub > 370-375 < /sub > fragment
This study offers for the first time of molecular insights into the S375G mutation affecting the aggregation of TDP-43370-375 at the atomic level, and may open new avenues in the development of future site-specific mutation therapeutics.PMID:38615537 | DOI:10.1016/j.bpc.2024.107230 (Source: Biophysical Chemistry)
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Zhengdong Xu Jianxin Zhang Jiaxing Tang Yehong Gong Yu Zou Qingwen Zhang Source Type: research
Inhibition of insulin fibrillation by carboxyphenylboronic acid-modified chitosan oligosaccharide based on electrostatic interactions and hydrophobic interactions
Biophys Chem. 2024 Apr 8;310:107236. doi: 10.1016/j.bpc.2024.107236. Online ahead of print.ABSTRACTA novel inhibitor, carboxyphenylboronic acid-modified chitosan oligosaccharide (COS-CPBA), was developed by coupling carboxyphenylboronic acid (CPBA) with chitosan oligosaccharide (COS) to inhibit insulin fibrillation. Extensive biophysical assays indicated that COS-CPBA could decelerate insulin aggregation, hinder the conformational transition from α-helix to β-sheet structure, change the morphology of insulin aggregates and alter fibrillation pathway. A mechanism for the inhibition of insulin fibrillation by COS-CPBA was ...
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Xiangyuan Zhao Chunyan Yang Wei Liu Ke Lu Hao Yin Source Type: research
Dissecting the effect of ALS mutation S375G on the conformational properties and aggregation dynamics of TDP-43 < sub > 370-375 < /sub > fragment
This study offers for the first time of molecular insights into the S375G mutation affecting the aggregation of TDP-43370-375 at the atomic level, and may open new avenues in the development of future site-specific mutation therapeutics.PMID:38615537 | DOI:10.1016/j.bpc.2024.107230 (Source: Biophysical Chemistry)
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Zhengdong Xu Jianxin Zhang Jiaxing Tang Yehong Gong Yu Zou Qingwen Zhang Source Type: research
Inhibition of insulin fibrillation by carboxyphenylboronic acid-modified chitosan oligosaccharide based on electrostatic interactions and hydrophobic interactions
Biophys Chem. 2024 Apr 8;310:107236. doi: 10.1016/j.bpc.2024.107236. Online ahead of print.ABSTRACTA novel inhibitor, carboxyphenylboronic acid-modified chitosan oligosaccharide (COS-CPBA), was developed by coupling carboxyphenylboronic acid (CPBA) with chitosan oligosaccharide (COS) to inhibit insulin fibrillation. Extensive biophysical assays indicated that COS-CPBA could decelerate insulin aggregation, hinder the conformational transition from α-helix to β-sheet structure, change the morphology of insulin aggregates and alter fibrillation pathway. A mechanism for the inhibition of insulin fibrillation by COS-CPBA was ...
Source: Biophysical Chemistry - April 14, 2024 Category: Chemistry Authors: Xiangyuan Zhao Chunyan Yang Wei Liu Ke Lu Hao Yin Source Type: research
Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation
This study investigates the inhibitory capacities of different Hsc70 variants, aiming to identify the structural determinants that strike a balance between efficacy and cytotoxicity. Our experimental findings demonstrate that the ATPase activity of Hsc70 is not a pivotal factor for inhibiting hIAPP misfolding. We underscore the significance of the C-terminal substrate-binding domain of Hsc70 in inhibiting hIAPP aggregation, emphasizing that the removal of the lid subdomain diminishes the inhibitory effect of Hsc70. Additionally, we employed atomistic discrete molecular dynamics simulations to gain deeper insights into the ...
Source: Biophysical Chemistry - April 12, 2024 Category: Chemistry Authors: Ali Chaari Nabanita Saikia Pradipta Paul Mohammad Yousef Feng Ding Moncef Ladjimi Source Type: research
Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation
This study investigates the inhibitory capacities of different Hsc70 variants, aiming to identify the structural determinants that strike a balance between efficacy and cytotoxicity. Our experimental findings demonstrate that the ATPase activity of Hsc70 is not a pivotal factor for inhibiting hIAPP misfolding. We underscore the significance of the C-terminal substrate-binding domain of Hsc70 in inhibiting hIAPP aggregation, emphasizing that the removal of the lid subdomain diminishes the inhibitory effect of Hsc70. Additionally, we employed atomistic discrete molecular dynamics simulations to gain deeper insights into the ...
Source: Biophysical Chemistry - April 12, 2024 Category: Chemistry Authors: Ali Chaari Nabanita Saikia Pradipta Paul Mohammad Yousef Feng Ding Moncef Ladjimi Source Type: research
Mechanistic insights into G-protein activation via phosphorylation mediated non-canonical pathway
Biophys Chem. 2024 Apr 5;309:107234. doi: 10.1016/j.bpc.2024.107234. Online ahead of print.ABSTRACTActivation of heterotrimeric G-proteins (Gαβγ) downstream to receptor tyrosine kinases (RTKs) is a well-established crosstalk between the signaling pathways mediated by G-protein coupled receptors (GPCRs) and RTKs. While GPCR serves as a guanine exchange factor (GEF) in the canonical activation of Gα that facilitates the exchange of GDP for GTP, the mechanism through which RTK phosphorylations induce Gα activation remains unclear. Recent experimental studies revealed that the epidermal growth factor receptor (EGFR), a we...
Source: Biophysical Chemistry - April 11, 2024 Category: Chemistry Authors: Kunal Shewani Midhun K Madhu Rajesh K Murarka Source Type: research
Mechanistic insights into G-protein activation via phosphorylation mediated non-canonical pathway
Biophys Chem. 2024 Apr 5;309:107234. doi: 10.1016/j.bpc.2024.107234. Online ahead of print.ABSTRACTActivation of heterotrimeric G-proteins (Gαβγ) downstream to receptor tyrosine kinases (RTKs) is a well-established crosstalk between the signaling pathways mediated by G-protein coupled receptors (GPCRs) and RTKs. While GPCR serves as a guanine exchange factor (GEF) in the canonical activation of Gα that facilitates the exchange of GDP for GTP, the mechanism through which RTK phosphorylations induce Gα activation remains unclear. Recent experimental studies revealed that the epidermal growth factor receptor (EGFR), a we...
Source: Biophysical Chemistry - April 11, 2024 Category: Chemistry Authors: Kunal Shewani Midhun K Madhu Rajesh K Murarka Source Type: research
