Serum albumin mitigated perfluorooctane sulfonate-induced cytotoxicity by affecting the cellular responses
Biophys Chem. 2023 Sep 18;302:107110. doi: 10.1016/j.bpc.2023.107110. Online ahead of print.ABSTRACTDuring the wide applications of perfluorinated materials such as perfluorooctane sulfonate (PFOS) in commercial and industrial products, the potential toxicity of these engineered compounds has attracted more and more attention. As a typical environmental pollutant, PFOS could preferentially bind to albumin protein in vivo. However, the role of protein-PFOS interactions in the cytotoxicity of PFOS was not stressed enough. Herein, we investigated the interactions of PFOS with human serum albumin (HSA, the most abundant protei...
Source: Biophysical Chemistry - September 23, 2023 Category: Chemistry Authors: Ya-Di Yang Jia-Xin Li Naihao Lu Rong Tian Source Type: research
Serum albumin mitigated perfluorooctane sulfonate-induced cytotoxicity by affecting the cellular responses
Biophys Chem. 2023 Sep 18;302:107110. doi: 10.1016/j.bpc.2023.107110. Online ahead of print.ABSTRACTDuring the wide applications of perfluorinated materials such as perfluorooctane sulfonate (PFOS) in commercial and industrial products, the potential toxicity of these engineered compounds has attracted more and more attention. As a typical environmental pollutant, PFOS could preferentially bind to albumin protein in vivo. However, the role of protein-PFOS interactions in the cytotoxicity of PFOS was not stressed enough. Herein, we investigated the interactions of PFOS with human serum albumin (HSA, the most abundant protei...
Source: Biophysical Chemistry - September 23, 2023 Category: Chemistry Authors: Ya-Di Yang Jia-Xin Li Naihao Lu Rong Tian Source Type: research
Effect of cycloastragenol and punicalagin on Prp(106-126) and A β(25-35) oligomerization and fibrillizaton
In this study, we have examined the effect of two NPs, cycloastragenol (CAG) and punicalagin (PCG), on the metal-induced oligomerization and aggregation of Aβ25-35 and PrP106-126 peptides. The peptide aggregation and inhibitory properties of both NPs were examined by the thioflavin-T (ThT) assay, MALDI-TOF, circular dichroism (CD) spectroscopy, and transmission electron microscopy (TEM). Among the two NPs, PCG significantly binds to the peptides, chelates metal ions (Cu2+ and Zn2+), inhibits peptide aggregation, substantially reduces oxidative stress, and controls the production of reactive oxygen species (ROS). Both NPs ...
Source: Biophysical Chemistry - September 21, 2023 Category: Chemistry Authors: Govinda R Navale Rahul Chauhan Saakshi Saini Partha Roy Kaushik Ghosh Source Type: research
Effect of cycloastragenol and punicalagin on Prp(106-126) and A β(25-35) oligomerization and fibrillizaton
In this study, we have examined the effect of two NPs, cycloastragenol (CAG) and punicalagin (PCG), on the metal-induced oligomerization and aggregation of Aβ25-35 and PrP106-126 peptides. The peptide aggregation and inhibitory properties of both NPs were examined by the thioflavin-T (ThT) assay, MALDI-TOF, circular dichroism (CD) spectroscopy, and transmission electron microscopy (TEM). Among the two NPs, PCG significantly binds to the peptides, chelates metal ions (Cu2+ and Zn2+), inhibits peptide aggregation, substantially reduces oxidative stress, and controls the production of reactive oxygen species (ROS). Both NPs ...
Source: Biophysical Chemistry - September 21, 2023 Category: Chemistry Authors: Govinda R Navale Rahul Chauhan Saakshi Saini Partha Roy Kaushik Ghosh Source Type: research
Effect of cycloastragenol and punicalagin on Prp(106-126) and A β(25-35) oligomerization and fibrillizaton
In this study, we have examined the effect of two NPs, cycloastragenol (CAG) and punicalagin (PCG), on the metal-induced oligomerization and aggregation of Aβ25-35 and PrP106-126 peptides. The peptide aggregation and inhibitory properties of both NPs were examined by the thioflavin-T (ThT) assay, MALDI-TOF, circular dichroism (CD) spectroscopy, and transmission electron microscopy (TEM). Among the two NPs, PCG significantly binds to the peptides, chelates metal ions (Cu2+ and Zn2+), inhibits peptide aggregation, substantially reduces oxidative stress, and controls the production of reactive oxygen species (ROS). Both NPs ...
Source: Biophysical Chemistry - September 21, 2023 Category: Chemistry Authors: Govinda R Navale Rahul Chauhan Saakshi Saini Partha Roy Kaushik Ghosh Source Type: research
Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation
Biophys Chem. 2023 Aug 22;302:107097. doi: 10.1016/j.bpc.2023.107097. Online ahead of print.ABSTRACTHigh temperature, acidic pH, and physical agitation are commonly observed during cooking or industrial food processing, which are often considered as favorable conditions, at least for some proteins, to misfold and form amyloid-like protein aggregates (APA). The proteins in various bakery products generally experience high temperatures that might lead to the formation of APA. To test this hypothesis, the presence of APA in white bread was examined in this study. The APA isolated from white bread displayed typical characteris...
Source: Biophysical Chemistry - September 12, 2023 Category: Chemistry Authors: Abhishek Singh Yadav Shweta Malik Indranil De Bhumika Pippal Manish Singh Neha Jain Jay Kant Yadav Source Type: research
Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation
Biophys Chem. 2023 Aug 22;302:107097. doi: 10.1016/j.bpc.2023.107097. Online ahead of print.ABSTRACTHigh temperature, acidic pH, and physical agitation are commonly observed during cooking or industrial food processing, which are often considered as favorable conditions, at least for some proteins, to misfold and form amyloid-like protein aggregates (APA). The proteins in various bakery products generally experience high temperatures that might lead to the formation of APA. To test this hypothesis, the presence of APA in white bread was examined in this study. The APA isolated from white bread displayed typical characteris...
Source: Biophysical Chemistry - September 12, 2023 Category: Chemistry Authors: Abhishek Singh Yadav Shweta Malik Indranil De Bhumika Pippal Manish Singh Neha Jain Jay Kant Yadav Source Type: research
Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation
Biophys Chem. 2023 Aug 22;302:107097. doi: 10.1016/j.bpc.2023.107097. Online ahead of print.ABSTRACTHigh temperature, acidic pH, and physical agitation are commonly observed during cooking or industrial food processing, which are often considered as favorable conditions, at least for some proteins, to misfold and form amyloid-like protein aggregates (APA). The proteins in various bakery products generally experience high temperatures that might lead to the formation of APA. To test this hypothesis, the presence of APA in white bread was examined in this study. The APA isolated from white bread displayed typical characteris...
Source: Biophysical Chemistry - September 12, 2023 Category: Chemistry Authors: Abhishek Singh Yadav Shweta Malik Indranil De Bhumika Pippal Manish Singh Neha Jain Jay Kant Yadav Source Type: research
Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation
Biophys Chem. 2023 Aug 22;302:107097. doi: 10.1016/j.bpc.2023.107097. Online ahead of print.ABSTRACTHigh temperature, acidic pH, and physical agitation are commonly observed during cooking or industrial food processing, which are often considered as favorable conditions, at least for some proteins, to misfold and form amyloid-like protein aggregates (APA). The proteins in various bakery products generally experience high temperatures that might lead to the formation of APA. To test this hypothesis, the presence of APA in white bread was examined in this study. The APA isolated from white bread displayed typical characteris...
Source: Biophysical Chemistry - September 12, 2023 Category: Chemistry Authors: Abhishek Singh Yadav Shweta Malik Indranil De Bhumika Pippal Manish Singh Neha Jain Jay Kant Yadav Source Type: research
Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation
Biophys Chem. 2023 Aug 22;302:107097. doi: 10.1016/j.bpc.2023.107097. Online ahead of print.ABSTRACTHigh temperature, acidic pH, and physical agitation are commonly observed during cooking or industrial food processing, which are often considered as favorable conditions, at least for some proteins, to misfold and form amyloid-like protein aggregates (APA). The proteins in various bakery products generally experience high temperatures that might lead to the formation of APA. To test this hypothesis, the presence of APA in white bread was examined in this study. The APA isolated from white bread displayed typical characteris...
Source: Biophysical Chemistry - September 12, 2023 Category: Chemistry Authors: Abhishek Singh Yadav Shweta Malik Indranil De Bhumika Pippal Manish Singh Neha Jain Jay Kant Yadav Source Type: research
Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation
Biophys Chem. 2023 Aug 22;302:107097. doi: 10.1016/j.bpc.2023.107097. Online ahead of print.ABSTRACTHigh temperature, acidic pH, and physical agitation are commonly observed during cooking or industrial food processing, which are often considered as favorable conditions, at least for some proteins, to misfold and form amyloid-like protein aggregates (APA). The proteins in various bakery products generally experience high temperatures that might lead to the formation of APA. To test this hypothesis, the presence of APA in white bread was examined in this study. The APA isolated from white bread displayed typical characteris...
Source: Biophysical Chemistry - September 12, 2023 Category: Chemistry Authors: Abhishek Singh Yadav Shweta Malik Indranil De Bhumika Pippal Manish Singh Neha Jain Jay Kant Yadav Source Type: research
Isolation of Amyloid-like Protein Aggregates (APA) from white bread and their characterisation
Biophys Chem. 2023 Aug 22;302:107097. doi: 10.1016/j.bpc.2023.107097. Online ahead of print.ABSTRACTHigh temperature, acidic pH, and physical agitation are commonly observed during cooking or industrial food processing, which are often considered as favorable conditions, at least for some proteins, to misfold and form amyloid-like protein aggregates (APA). The proteins in various bakery products generally experience high temperatures that might lead to the formation of APA. To test this hypothesis, the presence of APA in white bread was examined in this study. The APA isolated from white bread displayed typical characteris...
Source: Biophysical Chemistry - September 12, 2023 Category: Chemistry Authors: Abhishek Singh Yadav Shweta Malik Indranil De Bhumika Pippal Manish Singh Neha Jain Jay Kant Yadav Source Type: research
Asymmetrical interactions between nanoparticles and proteins arising from deformation upon adsorption to surfaces
Biophys Chem. 2023 Aug 23;302:107098. doi: 10.1016/j.bpc.2023.107098. Online ahead of print.ABSTRACTDrug release from polymeric nanoparticles (NPs) is governed by their adsorption onto cell membranes and transmigration across cell walls. These steps are influenced by their interactions with proteins near the cells. These interactions were investigated by studying the sequential adsorption of plasma proteins, albumin (Alb) and fibrinogen (Fg), and micellar NPs using quartz crystal microbalance with dissipation (QCMD), X-ray photoelectron spectroscopy (XPS), and small-angle X-ray scattering (SAXS). The three NPs in the study...
Source: Biophysical Chemistry - September 7, 2023 Category: Chemistry Authors: Megan Maniar Joachim Kohn N Sanjeeva Murthy Source Type: research
Asymmetrical interactions between nanoparticles and proteins arising from deformation upon adsorption to surfaces
Biophys Chem. 2023 Aug 23;302:107098. doi: 10.1016/j.bpc.2023.107098. Online ahead of print.ABSTRACTDrug release from polymeric nanoparticles (NPs) is governed by their adsorption onto cell membranes and transmigration across cell walls. These steps are influenced by their interactions with proteins near the cells. These interactions were investigated by studying the sequential adsorption of plasma proteins, albumin (Alb) and fibrinogen (Fg), and micellar NPs using quartz crystal microbalance with dissipation (QCMD), X-ray photoelectron spectroscopy (XPS), and small-angle X-ray scattering (SAXS). The three NPs in the study...
Source: Biophysical Chemistry - September 7, 2023 Category: Chemistry Authors: Megan Maniar Joachim Kohn N Sanjeeva Murthy Source Type: research
Asymmetrical interactions between nanoparticles and proteins arising from deformation upon adsorption to surfaces
Biophys Chem. 2023 Aug 23;302:107098. doi: 10.1016/j.bpc.2023.107098. Online ahead of print.ABSTRACTDrug release from polymeric nanoparticles (NPs) is governed by their adsorption onto cell membranes and transmigration across cell walls. These steps are influenced by their interactions with proteins near the cells. These interactions were investigated by studying the sequential adsorption of plasma proteins, albumin (Alb) and fibrinogen (Fg), and micellar NPs using quartz crystal microbalance with dissipation (QCMD), X-ray photoelectron spectroscopy (XPS), and small-angle X-ray scattering (SAXS). The three NPs in the study...
Source: Biophysical Chemistry - September 7, 2023 Category: Chemistry Authors: Megan Maniar Joachim Kohn N Sanjeeva Murthy Source Type: research
