Morphological control and modern applications of bicelles
Biophys Chem. 2023 Aug 19;302:107094. doi: 10.1016/j.bpc.2023.107094. Online ahead of print.ABSTRACTBicellar systems have become popularized as their rich morphology can be applied in biochemistry, physical chemistry, and drug delivery technology. To the biochemical field, bicelles are powerful model membranes for the study of transmembrane protein behavior, membrane transport, and environmental interactions with the cell. Their morphological responses to environmental changes reveal a profound fundamental understanding of physical chemistry related to the principle of self-assembly. Recently, they have also drawn signific...
Source: Biophysical Chemistry - September 2, 2023 Category: Chemistry Authors: Justin Amengual Luke Notaro-Roberts Mu-Ping Nieh Source Type: research
Morphological control and modern applications of bicelles
Biophys Chem. 2023 Aug 19;302:107094. doi: 10.1016/j.bpc.2023.107094. Online ahead of print.ABSTRACTBicellar systems have become popularized as their rich morphology can be applied in biochemistry, physical chemistry, and drug delivery technology. To the biochemical field, bicelles are powerful model membranes for the study of transmembrane protein behavior, membrane transport, and environmental interactions with the cell. Their morphological responses to environmental changes reveal a profound fundamental understanding of physical chemistry related to the principle of self-assembly. Recently, they have also drawn signific...
Source: Biophysical Chemistry - September 2, 2023 Category: Chemistry Authors: Justin Amengual Luke Notaro-Roberts Mu-Ping Nieh Source Type: research
Structural insights on the deformations induced by various mutations on cholesteryl ester transfer protein
Biophys Chem. 2023 Aug 15;301:107093. doi: 10.1016/j.bpc.2023.107093. Online ahead of print.ABSTRACTCholesteryl Ester Transfer Protein (CETP) is a plasma glycoprotein that intervenes the reverse cholesterol transport (RCT) by equimolar exchange of Cholesteryl esters (CE) and Triglycerides (TGs) between anti-atherogenic High-Density Lipoproteins (HDLs) and pro-atherogenic Low-Density Lipoproteins (LDLs) resulting in the increased concentration of CEs in LDL. This is a potential cause for the formation of atherosclerotic plaques in blood vessels leading to fatality. Therefore, blocking the function of CETP has emerged as a n...
Source: Biophysical Chemistry - August 28, 2023 Category: Chemistry Authors: Prasanna D Revanasiddappa Source Type: research
Structural insights on the deformations induced by various mutations on cholesteryl ester transfer protein
Biophys Chem. 2023 Aug 15;301:107093. doi: 10.1016/j.bpc.2023.107093. Online ahead of print.ABSTRACTCholesteryl Ester Transfer Protein (CETP) is a plasma glycoprotein that intervenes the reverse cholesterol transport (RCT) by equimolar exchange of Cholesteryl esters (CE) and Triglycerides (TGs) between anti-atherogenic High-Density Lipoproteins (HDLs) and pro-atherogenic Low-Density Lipoproteins (LDLs) resulting in the increased concentration of CEs in LDL. This is a potential cause for the formation of atherosclerotic plaques in blood vessels leading to fatality. Therefore, blocking the function of CETP has emerged as a n...
Source: Biophysical Chemistry - August 28, 2023 Category: Chemistry Authors: Prasanna D Revanasiddappa Source Type: research
Structural insights on the deformations induced by various mutations on cholesteryl ester transfer protein
Biophys Chem. 2023 Aug 15;301:107093. doi: 10.1016/j.bpc.2023.107093. Online ahead of print.ABSTRACTCholesteryl Ester Transfer Protein (CETP) is a plasma glycoprotein that intervenes the reverse cholesterol transport (RCT) by equimolar exchange of Cholesteryl esters (CE) and Triglycerides (TGs) between anti-atherogenic High-Density Lipoproteins (HDLs) and pro-atherogenic Low-Density Lipoproteins (LDLs) resulting in the increased concentration of CEs in LDL. This is a potential cause for the formation of atherosclerotic plaques in blood vessels leading to fatality. Therefore, blocking the function of CETP has emerged as a n...
Source: Biophysical Chemistry - August 28, 2023 Category: Chemistry Authors: Prasanna D Revanasiddappa Source Type: research
Structural insights on the deformations induced by various mutations on cholesteryl ester transfer protein
Biophys Chem. 2023 Aug 15;301:107093. doi: 10.1016/j.bpc.2023.107093. Online ahead of print.ABSTRACTCholesteryl Ester Transfer Protein (CETP) is a plasma glycoprotein that intervenes the reverse cholesterol transport (RCT) by equimolar exchange of Cholesteryl esters (CE) and Triglycerides (TGs) between anti-atherogenic High-Density Lipoproteins (HDLs) and pro-atherogenic Low-Density Lipoproteins (LDLs) resulting in the increased concentration of CEs in LDL. This is a potential cause for the formation of atherosclerotic plaques in blood vessels leading to fatality. Therefore, blocking the function of CETP has emerged as a n...
Source: Biophysical Chemistry - August 28, 2023 Category: Chemistry Authors: Prasanna D Revanasiddappa Source Type: research
Structural insights on the deformations induced by various mutations on cholesteryl ester transfer protein
Biophys Chem. 2023 Aug 15;301:107093. doi: 10.1016/j.bpc.2023.107093. Online ahead of print.ABSTRACTCholesteryl Ester Transfer Protein (CETP) is a plasma glycoprotein that intervenes the reverse cholesterol transport (RCT) by equimolar exchange of Cholesteryl esters (CE) and Triglycerides (TGs) between anti-atherogenic High-Density Lipoproteins (HDLs) and pro-atherogenic Low-Density Lipoproteins (LDLs) resulting in the increased concentration of CEs in LDL. This is a potential cause for the formation of atherosclerotic plaques in blood vessels leading to fatality. Therefore, blocking the function of CETP has emerged as a n...
Source: Biophysical Chemistry - August 28, 2023 Category: Chemistry Authors: Prasanna D Revanasiddappa Source Type: research
Ferguson plot analysis of multiple intermediate species of thermally unfolded bovine serum albumin
Biophys Chem. 2023 Aug 15;301:107095. doi: 10.1016/j.bpc.2023.107095. Online ahead of print.ABSTRACTFerguson plot was used to characterize the multiple intermediate species of bovine serum albumin (BSA) upon thermal unfolding. Differential scanning calorimetry showed an irreversible melting of BSA in Tris-HCl and phosphate buffers with a mid-transition temperature, Tm, of ∼68 °C. Thermally unfolded BSA was analyzed by agarose native gel electrophoresis stained by Coomassie blue and SYPRO Orange staining as a function of pH or protein concentration. SYPRO Orange was used to stain unfolded proteins. BSA heated at 70 and 8...
Source: Biophysical Chemistry - August 23, 2023 Category: Chemistry Authors: Yui Tomioka Satoru Nagatoishi Masataka Nakagawa Kouhei Tsumoto Tsutomu Arakawa Teruo Akuta Source Type: research
Ferguson plot analysis of multiple intermediate species of thermally unfolded bovine serum albumin
Biophys Chem. 2023 Aug 15;301:107095. doi: 10.1016/j.bpc.2023.107095. Online ahead of print.ABSTRACTFerguson plot was used to characterize the multiple intermediate species of bovine serum albumin (BSA) upon thermal unfolding. Differential scanning calorimetry showed an irreversible melting of BSA in Tris-HCl and phosphate buffers with a mid-transition temperature, Tm, of ∼68 °C. Thermally unfolded BSA was analyzed by agarose native gel electrophoresis stained by Coomassie blue and SYPRO Orange staining as a function of pH or protein concentration. SYPRO Orange was used to stain unfolded proteins. BSA heated at 70 and 8...
Source: Biophysical Chemistry - August 23, 2023 Category: Chemistry Authors: Yui Tomioka Satoru Nagatoishi Masataka Nakagawa Kouhei Tsumoto Tsutomu Arakawa Teruo Akuta Source Type: research
Ferguson plot analysis of multiple intermediate species of thermally unfolded bovine serum albumin
Biophys Chem. 2023 Aug 15;301:107095. doi: 10.1016/j.bpc.2023.107095. Online ahead of print.ABSTRACTFerguson plot was used to characterize the multiple intermediate species of bovine serum albumin (BSA) upon thermal unfolding. Differential scanning calorimetry showed an irreversible melting of BSA in Tris-HCl and phosphate buffers with a mid-transition temperature, Tm, of ∼68 °C. Thermally unfolded BSA was analyzed by agarose native gel electrophoresis stained by Coomassie blue and SYPRO Orange staining as a function of pH or protein concentration. SYPRO Orange was used to stain unfolded proteins. BSA heated at 70 and 8...
Source: Biophysical Chemistry - August 23, 2023 Category: Chemistry Authors: Yui Tomioka Satoru Nagatoishi Masataka Nakagawa Kouhei Tsumoto Tsutomu Arakawa Teruo Akuta Source Type: research
Ferguson plot analysis of multiple intermediate species of thermally unfolded bovine serum albumin
Biophys Chem. 2023 Aug 15;301:107095. doi: 10.1016/j.bpc.2023.107095. Online ahead of print.ABSTRACTFerguson plot was used to characterize the multiple intermediate species of bovine serum albumin (BSA) upon thermal unfolding. Differential scanning calorimetry showed an irreversible melting of BSA in Tris-HCl and phosphate buffers with a mid-transition temperature, Tm, of ∼68 °C. Thermally unfolded BSA was analyzed by agarose native gel electrophoresis stained by Coomassie blue and SYPRO Orange staining as a function of pH or protein concentration. SYPRO Orange was used to stain unfolded proteins. BSA heated at 70 and 8...
Source: Biophysical Chemistry - August 23, 2023 Category: Chemistry Authors: Yui Tomioka Satoru Nagatoishi Masataka Nakagawa Kouhei Tsumoto Tsutomu Arakawa Teruo Akuta Source Type: research
Lee's transient protonic capacitor cannot explain the surface proton current observed in bacteriorhodopsin purple membranes
Biophys Chem. 2023 Aug 15;301:107096. doi: 10.1016/j.bpc.2023.107096. Online ahead of print.ABSTRACTRecently in this Journal, James Lee employed his transmembrane electrostatically localized proton (TELP) hypothesis and the notion of a transient protonic capacitor to explain the force holding protons at the surface of bacteriorhodopsin purple membrane fragments. Here we show that purple membrane fragments cannot maintain the requisite transient non-zero transmembrane potential, and even if they could, it would not support the surface proton current moving from the P side to the N side that was reported by Heberle et al. (N...
Source: Biophysical Chemistry - August 21, 2023 Category: Chemistry Authors: Todd P Silverstein Source Type: research
Lee's transient protonic capacitor cannot explain the surface proton current observed in bacteriorhodopsin purple membranes
Biophys Chem. 2023 Aug 15;301:107096. doi: 10.1016/j.bpc.2023.107096. Online ahead of print.ABSTRACTRecently in this Journal, James Lee employed his transmembrane electrostatically localized proton (TELP) hypothesis and the notion of a transient protonic capacitor to explain the force holding protons at the surface of bacteriorhodopsin purple membrane fragments. Here we show that purple membrane fragments cannot maintain the requisite transient non-zero transmembrane potential, and even if they could, it would not support the surface proton current moving from the P side to the N side that was reported by Heberle et al. (N...
Source: Biophysical Chemistry - August 21, 2023 Category: Chemistry Authors: Todd P Silverstein Source Type: research
Direct interaction between the transmembrane helices stabilize cytochrome P450 2B4 and cytochrome b5 redox complex
Biophys Chem. 2023 Aug 8;301:107092. doi: 10.1016/j.bpc.2023.107092. Online ahead of print.ABSTRACTThe catalytic activity of cytochrome P450 2B4 (CYP2B4) is moderated by its cognate redox partner cytochrome b5 (Cyt-b5). The endoplasmic reticulum (ER) membrane and intermolecular transmembrane (TM) interaction between CYP2B4 and Cyt-b5 regulate the substrate catalysis and the reaction rate. This emphasizes the significance of elucidating the molecular basis of CYP2B4 and Cyt-b5 complexation in a membrane environment to better understand the enzymatic activity of CYP2B4. Our previous solid-state NMR studies revealed the membr...
Source: Biophysical Chemistry - August 16, 2023 Category: Chemistry Authors: Bikash R Sahoo Ayyalusamy Ramamoorthy Source Type: research
Direct interaction between the transmembrane helices stabilize cytochrome P450 2B4 and cytochrome b5 redox complex
Biophys Chem. 2023 Aug 8;301:107092. doi: 10.1016/j.bpc.2023.107092. Online ahead of print.ABSTRACTThe catalytic activity of cytochrome P450 2B4 (CYP2B4) is moderated by its cognate redox partner cytochrome b5 (Cyt-b5). The endoplasmic reticulum (ER) membrane and intermolecular transmembrane (TM) interaction between CYP2B4 and Cyt-b5 regulate the substrate catalysis and the reaction rate. This emphasizes the significance of elucidating the molecular basis of CYP2B4 and Cyt-b5 complexation in a membrane environment to better understand the enzymatic activity of CYP2B4. Our previous solid-state NMR studies revealed the membr...
Source: Biophysical Chemistry - August 16, 2023 Category: Chemistry Authors: Bikash R Sahoo Ayyalusamy Ramamoorthy Source Type: research
