The evolutionary pattern of cocaine and hyoscyamine biosynthesis provides strategies to produce tropane alkaloids
Chembiochem. 2023 May 30:e202300234. doi: 10.1002/cbic.202300234. Online ahead of print.ABSTRACTCocaine and hyoscyamine are two tropane alkaloids (TA) from Erythroxylaceae and Solanaceae, respectively. These famous compounds possess anticholinergic properties that can be used to treat neuromuscular disorders. While the hyoscyamine biosynthetic pathway has been fully elucidated allowing its de novo synthesis in yeast, the cocaine pathway remained partially elucidated. Recently, the Huang research group has completed the cocaine biosynthetic route by characterizing its two missing enzymes. This allowed transferring this whol...
Source: Chembiochem - May 30, 2023 Category: Biochemistry Authors: Duchesse-Lacours Zamar Nicolas Papon Vincent Courdavault Source Type: research
Recycling of hyoscyamine 6 β-hydroxylase for the in vitro production of anisodamine and scopolamine
Appl Microbiol Biotechnol. 2023 Apr 26. doi: 10.1007/s00253-023-12537-w. Online ahead of print.ABSTRACTThe tropane alkaloids hyoscyamine, anisodamine, and scopolamine are extensively used medicines. In particular, scopolamine has the greatest value in the market. Hence, strategies to enhance its production have been explored as an alternative to traditional field-plant cultivation. In this work, we developed biocatalytic strategies for the transformation of hyoscyamine into its products utilizing a recombinant Hyoscyamine 6β-hydroxylase (H6H) fusion protein to the chitin-binding domain of the chitinase A1 from Bacillus su...
Source: Applied Microbiology and Biotechnology - April 26, 2023 Category: Microbiology Authors: Juan M Minoia Mar ía E Villanueva Guillermo J Copello Juli án Rodríguez Talou Alejandra B Cardillo Source Type: research
Recycling of hyoscyamine 6 β-hydroxylase for the in vitro production of anisodamine and scopolamine
Appl Microbiol Biotechnol. 2023 Apr 26. doi: 10.1007/s00253-023-12537-w. Online ahead of print.ABSTRACTThe tropane alkaloids hyoscyamine, anisodamine, and scopolamine are extensively used medicines. In particular, scopolamine has the greatest value in the market. Hence, strategies to enhance its production have been explored as an alternative to traditional field-plant cultivation. In this work, we developed biocatalytic strategies for the transformation of hyoscyamine into its products utilizing a recombinant Hyoscyamine 6β-hydroxylase (H6H) fusion protein to the chitin-binding domain of the chitinase A1 from Bacillus su...
Source: Applied Microbiology and Biotechnology - April 26, 2023 Category: Microbiology Authors: Juan M Minoia Mar ía E Villanueva Guillermo J Copello Juli án Rodríguez Talou Alejandra B Cardillo Source Type: research
MIL-53(Fe)-derived ant nest structured porous carbon nanospheres CuFeS < sub > 2 < /sub > /C for the determination of atropine enantiomeric impurity L-hyoscyamine
In conclusion, the sensor showed excellent reproducibility, repeatability, and stability and had been applied to the determination of L-HSM in human serum, saliva, and ATP sulfate tablets with satisfactory results.PMID:36190759 | DOI:10.1002/chir.23500 (Source: Chirality)
Source: Chirality - October 3, 2022 Category: Molecular Biology Authors: Jiashuai Zhu Kang Li Source Type: research
MIL-53(Fe)-derived ant nest structured porous carbon nanospheres CuFeS < sub > 2 < /sub > /C for the determination of atropine enantiomeric impurity L-hyoscyamine
In conclusion, the sensor showed excellent reproducibility, repeatability, and stability and had been applied to the determination of L-HSM in human serum, saliva, and ATP sulfate tablets with satisfactory results.PMID:36190759 | DOI:10.1002/chir.23500 (Source: Chirality)
Source: Chirality - October 3, 2022 Category: Molecular Biology Authors: Jiashuai Zhu Kang Li Source Type: research
MIL-53(Fe)-derived ant nest structured porous carbon nanospheres CuFeS < sub > 2 < /sub > /C for the determination of atropine enantiomeric impurity L-hyoscyamine
In conclusion, the sensor showed excellent reproducibility, repeatability, and stability and had been applied to the determination of L-HSM in human serum, saliva, and ATP sulfate tablets with satisfactory results.PMID:36190759 | DOI:10.1002/chir.23500 (Source: Chirality)
Source: Chirality - October 3, 2022 Category: Molecular Biology Authors: Jiashuai Zhu Kang Li Source Type: research
MIL-53(Fe)-derived ant nest structured porous carbon nanospheres CuFeS < sub > 2 < /sub > /C for the determination of atropine enantiomeric impurity L-hyoscyamine
In conclusion, the sensor showed excellent reproducibility, repeatability, and stability and had been applied to the determination of L-HSM in human serum, saliva, and ATP sulfate tablets with satisfactory results.PMID:36190759 | DOI:10.1002/chir.23500 (Source: Chirality)
Source: Chirality - October 3, 2022 Category: Molecular Biology Authors: Jiashuai Zhu Kang Li Source Type: research
MIL-53(Fe)-derived ant nest structured porous carbon nanospheres CuFeS < sub > 2 < /sub > /C for the determination of atropine enantiomeric impurity L-hyoscyamine
In conclusion, the sensor showed excellent reproducibility, repeatability, and stability and had been applied to the determination of L-HSM in human serum, saliva, and ATP sulfate tablets with satisfactory results.PMID:36190759 | DOI:10.1002/chir.23500 (Source: Chirality)
Source: Chirality - October 3, 2022 Category: Molecular Biology Authors: Jiashuai Zhu Kang Li Source Type: research
MIL-53(Fe)-derived ant nest structured porous carbon nanospheres CuFeS < sub > 2 < /sub > /C for the determination of atropine enantiomeric impurity L-hyoscyamine
In conclusion, the sensor showed excellent reproducibility, repeatability, and stability and had been applied to the determination of L-HSM in human serum, saliva, and ATP sulfate tablets with satisfactory results.PMID:36190759 | DOI:10.1002/chir.23500 (Source: Chirality)
Source: Chirality - October 3, 2022 Category: Molecular Biology Authors: Jiashuai Zhu Kang Li Source Type: research
MIL-53(Fe)-derived ant nest structured porous carbon nanospheres CuFeS < sub > 2 < /sub > /C for the determination of atropine enantiomeric impurity L-hyoscyamine
In conclusion, the sensor showed excellent reproducibility, repeatability, and stability and had been applied to the determination of L-HSM in human serum, saliva, and ATP sulfate tablets with satisfactory results.PMID:36190759 | DOI:10.1002/chir.23500 (Source: Chirality)
Source: Chirality - October 3, 2022 Category: Molecular Biology Authors: Jiashuai Zhu Kang Li Source Type: research
A validated LC-MS/MS method for the determination of L-hyoscyamine in human plasma: Application in clinical study
In this study, a new sensitive, stable, and selective LC/MS assay was developed for determination of L-hyoscyamine and applied to the clinical study. The parent-product (m/z) transition pair of L-hyoscyamine was 290.1→124.1. Chromatographic separations were performed using a Chiral MZ column (250 mm×4.6 mm, 5.0 μm) by a stepwise gradient elution mode with n-hexane, isopropanol and diethylamine as mobile phases. L-hyoscyamine in human plasma were extracted using liquid-liquid extraction process. This assay displayed a good linearity over a concentration range of 20.0-400 pg/mL for L-hyoscyamine. The accuracy of the vali...
Source: Biomedical Chromatography : BMC - August 23, 2022 Category: Biomedical Science Authors: Yunhai Duan Zhenglong Zuo Yinchun Chen Qingqing Song Mingming Yu Yong Yang Source Type: research
A validated LC-MS/MS method for the determination of L-hyoscyamine in human plasma: Application in clinical study
In this study, a new sensitive, stable, and selective LC/MS assay was developed for determination of L-hyoscyamine and applied to the clinical study. The parent-product (m/z) transition pair of L-hyoscyamine was 290.1→124.1. Chromatographic separations were performed using a Chiral MZ column (250 mm×4.6 mm, 5.0 μm) by a stepwise gradient elution mode with n-hexane, isopropanol and diethylamine as mobile phases. L-hyoscyamine in human plasma were extracted using liquid-liquid extraction process. This assay displayed a good linearity over a concentration range of 20.0-400 pg/mL for L-hyoscyamine. The accuracy of the vali...
Source: Biomedical Chromatography : BMC - August 23, 2022 Category: Biomedical Science Authors: Yunhai Duan Zhenglong Zuo Yinchun Chen Qingqing Song Mingming Yu Yong Yang Source Type: research
Fluorescence-based immunochromatographic test strip for the detection of hyoscyamine
Analyst, 2022, Advance Article DOI: 10.1039/D1AN01973B, PaperXinxin Xu, Wenliang Ge, Steven Suryoprabowo, Xin Guo, Jianping Zhu, Liqiang Liu, Chuanlai Xu, Hua Kuang A fluorescent microsphere based immunochromatographic assay was developed for hyoscyamine. In comparison with a AuNP immunochromatographic assay, the developed method offered a higher coupling rate and lower amounts of antibodies. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Analyst latest articles)
Source: RSC - Analyst latest articles - December 15, 2021 Category: Chemistry Authors: Xinxin Xu Source Type: research
Fluorescence-based Immunochromatographic strip test for the detection of hyoscyamine
Analyst, 2022, Accepted Manuscript DOI: 10.1039/D1AN01973B, PaperXin-Xin Xu, wenliang ge, Steven Suryoprabowo, xin guo, jianping zhu, Liqiang Liu, Chuanlai Xu, Hua Kuang Hyoscyamine (HSM) which act as antagonists of acetylcholine muscarinic receptor and can induce a variety of distinct toxic syndromes in mammals (anti-cholinergic poisoning) which hazardous in human health. Therefore, it... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Analyst latest articles)
Source: RSC - Analyst latest articles - December 1, 2021 Category: Chemistry Authors: Xin-Xin Xu Source Type: research
CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots
In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.PMID:34562094 | DOI:10.1093/bbb/zbab165 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - September 25, 2021 Category: Biochemistry Authors: Fumihito Hasebe Honoka Yuba Takashi Hashimoto Kazuki Saito Nobutaka Funa Tsubasa Shoji Source Type: research
