Structural characterization and biological evaluation of a new O-acetyl-1,4-linked- β-d-mannan possessed potential application in hydrophilic polymer materials from Dendrobium devonianum

Int J Biol Macromol. 2022 May 17:S0141-8130(22)01072-8. doi: 10.1016/j.ijbiomac.2022.05.098. Online ahead of print.ABSTRACTTo explore the active polysaccharides from Dendrobium devonianum, a novel O-acetylmannan (DDP-1) with molecular weight of 117 kDa was isolated from D. devonianum. The chemical and instrumental analysis indicated that the DDP-1 was a homopolysaccharide containing a backbone chain composed of →4)-β-d-Manp-(1 → (71.4%) residue with internal →4)-2-O-acetyl-β-d-Manp-(1 → (14.2%), →4)-3-O-acetyl-β-d-Manp-(1 → (7.1%), and non-reducing end β-d-Manp-(1 → (7.3%) residues. Anticancer assay in vitro revealed that DDP-1 had anticancer activity against the growth of HepG2 and MCF-7 cancer cells. Moreover, cytokine secretion assays also presented that DDP-1 can promote cytokine production of TNF-α and IL-6 in THP-1 macrophage stimulated by PMA. Finally, the effects of isolation and purification on the microstructure of DDP-1 was studied by scanning electron microscope. The morphological features of DDP-1 indicated that DDP-1 hold high potential application in hydrophilic polymer materials.PMID:35594938 | DOI:10.1016/j.ijbiomac.2022.05.098
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Source Type: research