Solid Lipid Nanoparticles of Lepidium Sativum L Seed Extract: Formulation, Optimization and In vitro Cytotoxicity Studies
Drug Res (Stuttg) DOI: 10.1055/a-1800-5956The current study focused on important bioactive compounds in plants that make
them pharmacologically valuable. Therefore, this study was aimed to develop
Lepidium sativum (L. sativum) seed extract loaded solid lipid
nanoparticles and explore its cytotoxic effect on human liver cancer cells
(HepG2 cells). The ethanolic extract of L. sativam used to develop L.
sativum seed extract loaded solid lipid nanoparticles (SLNs) was
analyzed by gas chromatography-mass spectrometry, thin-layer chromatography
(TLC) and high-performance thin-layer chromatography (HPTLC) for phytochemical
profiling. The L. sativum seed extract loaded SLNs were efficaciously
prepared by the nanoprecipitation method and screened on the basis of
physicochemical properties. The L. sativum seed extract loaded SLN-2 was
characterized using various parameters like particle size (237.1±0.104),
% entrapment efficiency (80±1.15), zeta potential
(42.1±0.102) and % drug release (45% at the end
8 hours and release the entire amount in 12 h). The SLN-2
formulation was optimized based on the recipient factor, and SLN-2 was used to
further evaluate the in vitro cytotoxicity of HepG2 cells in a dose-dependent
manner by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT)
assay. T...
Source: Drug Research - Category: Drugs & Pharmacology Authors: Ahmad, Asad Tabassum, Heena Nabi, Rabia Mishra, Anuradha Ahmad, Iffat Zareen Tags: Original Article Source Type: research
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