Identification of Sphingomonas paucimobolis from a case of soft tissue injury, based on the phenotypic characteristics and 16S rRNA gene sequence

Soft tissue injuries can result in pain, swelling, bruising and loss of function. A large area of open soft tissue injury, opened the channel for the invasion of pathogen, so bloodstream infections as a result of soft tissue injury in the clinical diagnosis and treatment are common, and also show an upward trend. Sphingomonas paucimobilis is a Gram-negative bacillus that is emerging as an opportunistic pathogen. It is widely found in nature, especially in water and soil, and has been isolated from hospital environments such as distilled water, nebulizers, and multiple equipments used in medical care. There are few published articles about the S. paucimobilis infections and majority of them are limited to case reports or reports with low number of cases. However, there is no published serial data about the infections related to S. paucimobilis in soft tissue injury. Meanwhile, some strains of the species could not be determined easily because some of their phenotypic characteristics did not correlate with the species descriptions published in the literature. Molecular methods, such as PCR amplification and DNA sequencing, are valuable in strain identification. In the study we identified S. paucimobilis from a soft tissue injury patient with blood culture-positive by phenotypic characteristics and 16S rRNA gene sequence.
Source: Reviews in Medical Microbiology - Category: Microbiology Tags: Case Presentations Source Type: research