Accelerated embryonic stem cell screening with a highly efficient genotyping pipeline

CONCLUSION: In sum, we describe a novel and effective implementation of ddPCR, multiMACS, and adenovirus recombinase into a streamlined screening workflow that significantly reduces timelines for gene targeting in mouse ES cells.PMID:35107736 | DOI:10.1007/s11033-022-07165-y

Source: Mol Biol Cell - February 2, 2022 Category: Molecular Biology Authors: Roger Caothien Charles Yu Lucinda Tam Robert Newman Brian Nakao Tuija Alcantar Natasha Bacarro Juan Reyes Anna Pham Merone Roose-Girma Source Type: research