GIT1 is an untolerized autoantigen involved in immunologic disturbance of spermatogenesis

In this study, we focused on G protein-coupled receptor kinase interacting protein-1 (GIT1), another haploid cell-specific differentiation antigen, to investigate whether GIT1 is a target autoantigen for EAO induction. GIT1 emulsified with complete Freund’s adjuvant was injected subcutan eously into the mice inguinal region once on day 0 and again on day 14, and the optimum condition of EAO induction was determined. Mice immunized with 200 μg GIT1 showed significantly higher incidence of EAO than that of immunization with other concentrations. In particular, significant lymphocyti c inflammation and extensive aspermatogenesis were observed in these mice at 120 days after the first immunization. These findings indicate that GIT1 is also a target antigen that induces EAO, like HSPA4L.
Source: Histochemistry and Cell Biology - Category: Biomedical Science Source Type: research