Studying Diabetic Retinopathy?

Check out How Neuromic ' s Tools Are UsedThe authors used four of Neuromics to complete this study-Sulodexide reduces glucose induced senescence in human retinal endothelial cellsA. Gericke, K. Suminska-Jasi ńska& A. Br ęborowicz Scientific Reports volume 11, Article number: 11532 (2021) Cite this article.Material and MethodsExperiments were performed on HREC (#HEC09, Neuromics, Edina, MN, USA) in in vitro culture. Cells were seeded in 75 cm2 culture flasks coated with AlphaBioCoat Solution (#AC00, Neuromics, Edina, MN, USA) and were grown in Endo Growth Medium (EKG001, Neuromics, Edina, MN, USA) supplemented with fetal bovine serum 0.5% until they formed monolayers. Then the cells were harvested with cell detachment solution (#ADF001, Neuromics, Edina, MN, USA) and seeded in quadruplicates into 25 cm2 coated, as described above, culture flasks at a density of 1.5  × 105 cells/flask.We conclude that Sulodexide may have a beneficial effect in cases of diabetic retinopathy. It slows down hyperglycemia-dependent senescence of endothelial cells, which translates into the lower angiogenic and inflammatory impact of these cells. An important observation was that Sulodexide also has effective antiangiogenic and anti-inflammatory effects in the senescent HREC. That means that Sulodexide may be effective in retinal endothelial cells, which are already senescent. Further studies are required to explain the potential effect of Sulodexide on the endothelial glycocalyx structur...
Source: Neuromics - Category: Neuroscience Tags: Cell Detachment Diabetic retinopathy Human Endothelial Cells Human Retinal Microvascular Endothelial Cells Hyperglycemia Source Type: news