3, 5-Di-tert-butylphenol combat against Streptococcus mutans by impeding acidogenicity, acidurance and biofilm formation

AbstractStreptococcus mutans is a common pathogen present in the oral cavity and it causes dental caries for all aged groups of people, in particular, children.S. mutans have several virulence factors such as acidogenecity, aciduricity, adhesion and biofilm formation. These virulence factors are working together and lead to the development of caries in the tooth surface. The present study aimed to investigate the anticariogenic potential of 3, 5-di-tert-butylphenol (3, 5-DTBP) againstS. mutans. 3, 5-DTBP biofilm inhibitory concentration (BIC) was found at 100  µg/ml concentration without any lethal effect on the growth. Moreover, 3, 5-DTBP significantly reduced water soluble and water insoluble glucans production, in concurrence with downregulation ofgtfBC genes. Moreover, acidogenicity associated virulence factors such as lactate dehydrogenase and enolase enzymatic production was arrested upon 3, 5-DTBP treatment. In addition, 3, 5-DTBP greatly reduced acidtolerance ability through impedes of F1F0-ATPase. Gene expression analysis unveiled the downregulation ofgtfB,gtfC,gtfD,vicRK,comDE,gbpB,smu0630 andrelA upon 3, 5-DTBP treatment. The present study paves the way for exhibiting 3, 5-DTBP as a promising therapeutic agent to controlS. mutans infections.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research