Tissue culture-based genetic improvement of fava bean (Vicia faba L.): analysis on previous achievements and future perspectives

Appl Microbiol Biotechnol. 2021 Aug 24. doi: 10.1007/s00253-021-11517-2. Online ahead of print.ABSTRACTFava bean is an extremely important legume and serves immense potential to function as an ingredient as pulse proteins in human diet. Bearing the proficiency of yielding magnanimous amount of functional and nutritional ingredients, this bean deserves to replace any other leguminous crop too. The instability of fava bean in its yield makes breeding for crop improvement difficult, and its high susceptibility to a number of abiotic and biotic stresses additionally results in unstable yields. The self-incompatibility leads to the formation of a limited genetic pool and shows a slow progress in breeding. The plant is highly recalcitrant, making it an onerous task to micropropagate or regenerate fava beans under in vitro conditions. Another fly in the ointment is the release of phenolic compounds by the plant. There are several endeavours that have been made to establish in vitro regeneration, protoplast culture, and genetic transformation and to genetically improve this plant. Nonetheless there are a number of promising cutting-edge technologies that are yet to be harnessed in order to ensure its comprehensive and sustainable genetic improvement. The in vitro-based technologies of this legume and its untraveled path in the plant tissue culture-mediated approaches can assist further genetic manipulation of this plant species in a smoother manner and at an exponential rate. Creatio...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Source Type: research