O-091 Semen microbiota in patients with astenozoospermia and healthy controls: cluster analysis of real-time PCR data

AbstractStudy questionWhat are the differences in the semen microbiota composition of patients with asthenozoospermia and normospermia according to cluster analysis of PCR data?Summary answerThe detection rate of 4 stable semen microbiota clusters and the dominant bacteria groups varied in patients with asthenozoospermia and normospermia.What is known alreadyMost of the research dedicated to analyzing normal and pathological semen microbiota is based on 16S rRNA gene specific Next generation sequencing (NGS). It has shown that microbiota is represented by polymicrobial communities (clusters) that consist of microorganisms from different genera and bacteria phyla. Despite it being highly informative, NGS has several weaknesses: complex sample preparation, difficult sample intake control, long analysis process, complicated results interpretation, high cost of equipment and reagents. These factors make it virtually impossible to use this approach in routine medical practice. Quantitative real-time PCR (RT-PCR) is far more suitable for this.Study design, size, durationPatients included in the study (n  = 301) came to the “Garmonia” Medical Center (Yekaterinburg, Russia) either seeking preconception care or for infertility treatment. Depending on the spermiogram results, they were divided into two groups. Group 1 (n = 171) — asthenozoospermia, Group 2 (n = 130) — normospermia.Participants/materials, setting, methodsSemen microbiota was analyzed using RT-PCR kit...
Source: Human Reproduction - Category: Reproduction Medicine Source Type: research