Application of serum and urinary electrophoresis as an aid tool for evaluating dogs at different stages of chronic kidney disease

AbstractChronic kidney disease (CKD) is a common, progressive, and irreversible disease in dogs. Inflammation and proteinuria are related to the process of CKD progression. The search for early markers and indicators of the degree and location of renal inflammation to aid in diagnosis and prognosis is very important. The aim of this study was to evaluate the applicability of serum and urinary electrophoresis as an aid tool in the diagnosis of dogs with chronic kidney disease according to the stage of CKD (IRIS2019b). For this, 27 canine patients from routine clinical practice were evaluated and divided into five groups: C (control), G1 (CKD —stage 1), G2 (CKD—stage 2), G3 (CKD—stage 3), and G4 (CKD—stage 4). Samples were evaluated by serum and urine biochemistry, urinalysis, and serum and urine polyacrylamide gel electrophoresis (SDS-PAGE). In serum electrophoresis, difference was observed between the C and the CKD group, with a decrease in 40.76 (P = 0.017), 33.96 (P = 0.025), and 25.79 kDa (P = 0.049) serum proteins caused by the loss of protein in the urine in dogs affected with CKD. The urinary electrophoresis showed an important difference between groups in the evaluation of the lesion pattern, and in the glomerular and tubular lesion score, there was a difference in the glomerular lesion score of C in relation to G2 (P = 0.007), G3 (P = 0.016), and G4 (P = 0.000). Regarding the tubular lesion score, there was a difference between C and G2 (P = 0.029), G3 (P = 0....
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research