Molecules, Vol. 26, Pages 4626: Cholesterol Sequestration from Caveolae/Lipid Rafts Enhances Cationic Liposome-Mediated Nucleic Acid Delivery into Endothelial Cells

In this study, we demonstrated that the transient modulation of caveolae/lipid rafts mediated endocytosis with the cholesterol-sequestrating agents, nystatin, filipin III, and siRNA against Cav-1, which significantly increased the transfection properties of cationic lipid-(2-hydroxy-N-methyl-N,N-bis(2-tetradecanamidoethyl)ethanaminium chloride), namely, amide liposomes in combination with 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) (AD Liposomes) in liver sinusoidal endothelial cells (SK-Hep1). In particular, nystatin was found to be highly effective with 2–3-fold enhanced transfection efficacy when compared with amide liposomes in combination with Cholesterol (AC), by switching lipoplex internalization predominantly through clathrin-mediated endocytosis and macropinocytosis.

Source: Molecules - July 30, 2021 Category: Chemistry Authors: Santhosh Chandar Maddila Chandrashekhar Voshavar Porkizhi Arjunan Rashmi Prakash Chowath Hari Krishna Reddy Rachamalla Balaji Balakrishnan Poonkuzhali Balasubramanian Rajkumar Banerjee Srujan Marepally Tags: Article Source Type: research