Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis

ConclusionNested PCR and GeneXpert ultra on formalin fixed paraffin embedded tissues are useful tools for the diagnosis of tuberculous spondylodiscitis, especially for cases where microbiological investigations were not carried out. Both techniques have excellent specificity but single tube nested PCR is more sensitive.Key Points• Molecular techniques are routinely performed on fresh tissues• GeneXpert and nested PCR on formalin fixed paraffin embedded tissues are reliable for the diagnosis of tuberculous spondylodiscitis• Nested PCR is more sensitive than Genexpert for diagnosing tuberculous spondylodiscitis

http://link.springer.com/10.1007/s10067-021-05782-9

Source: Clinical Rheumatology - June 7, 2021 Category: Rheumatology Source Type: research