Gene and protein expression in human megakaryocytes derived from induced pluripotent stem cells

Conclusions MKs expressed genes and proteins known to be important in MK and platelet function and demonstrated good agreement with previous studies in human MKs derived from CD34+ progenitor cells. The percent of cells expressing the MK markers CD41 and CD42a was consistent in biological replicates, but variable across subjects, suggesting that unidentified subject-specific factors determine differentiation of MKs from iPSCs. Gene and protein sets important in platelet function were associated with increasing expression of CD41/42a, while those related to more basic cellular functions were associated with lower CD41/42a expression. There was differential gene expression by the sex and race (but not age) of the subject. Numerous genes and proteins were highly expressed in MKs but not known to play a role in MK or platelet function; these represent excellent candidates for future study of hematopoiesis, platelet formation, and/or platelet function.PMID:33829634 | DOI:10.1111/jth.15334

Source: Thrombosis and Haemostasis - April 8, 2021 Category: Hematology Authors: Kai Kammers Margaret A Taub Rasika A Mathias Lisa R Yanek Kanika Kanchan Vidya Venkatraman Niveda Sundararaman Joshua Martin Senquan Liu Dixie Hoyle Koen Raedschelders Ronald Holewinski Sarah Parker Victoria Dardov Nauder Faraday Diane M Becker Linzhao Ch Source Type: research