DIP2A is involved in SOD-mediated antioxidative reactions in murine brain

Free Radic Biol Med. 2021 Mar 26:S0891-5849(21)00187-8. doi: 10.1016/j.freeradbiomed.2021.03.027. Online ahead of print.ABSTRACTAutism spectrum disorders (ASDs) are highly associated with oxidative stress. We have recently shown that Disconnected-interacting protein homolog 2 A (DIP2A) functions in ASD pathophysiology by regulating cortactin acetylation for spine development and synaptic transmission. However, its role is not fully understood in the context of its abundant expression in mitochondria. In this paper, we found that DIP2A was involved in superoxide dismutase (SOD)-mediated antioxidative reactions. In mice, DIP2A knockout inhibited SOD activity and increased reactive oxygen species (ROS) levels in the cerebral cortex. In vitro gain-of-function experiments further confirmed the positive role of DIP2A in scavenging ROS upon oxidative stress. Moreover, DIP2A knockout caused irregular mitochondrial morphology in the cerebral cortex and impaired mitochondrial metabolism with an over consumption of lipids for energy supply. Taken together, these results revealed unrecognized functions of DIP2A in antioxidative protection, providing another possible explanation for DIP2A-mediated ASD pathophysiology.PMID:33781892 | DOI:10.1016/j.freeradbiomed.2021.03.027
Source: Free Radical Biology and Medicine - Category: Biology Authors: Source Type: research