Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2

J Microbiol Immunol Infect. 2021 Feb 1:S1684-1182(21)00019-0. doi: 10.1016/j.jmii.2021.01.005. Online ahead of print.ABSTRACTTo determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%-100%) and the negative percentage agreement was 97.1% (67 of 69; 95% CI, 93.1%-100%).PMID:33582073 | PMC:PMC7849545 | DOI:10.1016/j.jmii.2021.01.005

Source: Journal of Microbiology, Immunology, and Infection - February 14, 2021 Category: Microbiology Authors: Pai-Ling Chang Chia-Yi Lin Cheng-Pin Chen Yi-Chun Lin Hui-Chun Hu Shu-Hsing Cheng Chien-Yu Cheng Source Type: research