PG1659 functions as anti ‐sigma factor to extracytoplasmic function sigma factor RpoE in Porphyromonas gingivalis W83

The sigma factor RopE plays an important role in oxidative stress resistance and the virulence regulatory network inP. gingivalis. Collectively, the data in this report have demonstrated the functional properties of anti ‐sigma factor PG1659 in its modulation of RopE inP. gingivalis. It is likely that PG1659 is involved in a complex regulatory network that may represent a yet ‐to‐be described oxidative stress resistance pathway inP. gingivalis. AbstractAnti ‐sigma factors play a critical role in regulating the expression of sigma factors in response to environmental stress signals.PG1659 is cotranscribed with an upstream genePG1660 (rpoE), which encodes for a sigma factor that plays an important role in oxidative stress resistance and the virulence regulatory network ofP.gingivalis.PG1659, which is annotated as a hypothetical gene, is evaluated in this study. PG1659, composed of 130 amino acids, is predicted to be transmembrane protein with a single calcium (Ca2+) binding site. InP.gingivalis FLL358 ( ΔPG1659::ermF), therpoE gene was highly upregulated compared to the wild ‐type W83 strain. RpoE‐induced genes were also upregulated in thePG1659‐defective isogenic mutant. Both protein‐protein pull‐down and bacterial two‐hybrid assays revealed that the PG1659 protein could interact with/bind RpoE. The N‐terminal domain of PG1659, representing the cytoplasmic fragment of the protein, is critical for interaction with RpoE. In the pr esence of PG1659, the ini...
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research