Exhaled breath condensate in acute pulmonary embolism; a porcine study of effect of condensing temperature and feasibility of protein analysis by mass spectrometry
The search for diagnostic biomarkers for pulmonary embolism (PE) has mainly been focused on blood
samples. Exhaled breath condensate (EBC) is a possible source for biomarkers specific for chronic
lung diseases and cancer, yet no previous studies have investigated the potential of EBC for
diagnosis of PE. The protein content in the EBC is very low, and efficient condensing of the EBC is
important in order to obtain high quality samples for protein analysis. We investigated if advanced
proteomic techniques in a porcine model of acute intermediate-high-risk PE was feasible using two
different condensing temperatures for EBC collection. Seven pigs were anaesthetized and intubated.
EBC was collected one hour after intubation. Two autologous emboli were induced through the right
external jugular vein. Two hours after the emboli were administered, EBC was collected again.
Condensing temperature was either −21 °C or −80 °C. Nano liquid chromatography-tandem mass
spectrometry (nLC-MS/MS)...
Source: Journal of Breath Research - Category: Respiratory Medicine Authors: Inger Lise Gade, Jacob Gammelgaard Schultz, Lasse J ørgensen Cehofski, Benedict Kjærgaard, Marianne Tang Severinsen, Bodil Steen Rasmussen, Henrik Vorum, Bent Honoré and Søren Risom Kristensen Source Type: research
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