EDTA as a chelating agent in quantitative 1H-NMR of biologically important ions.

EDTA as a chelating agent in quantitative 1H-NMR of biologically important ions. Biochem Cell Biol. 2021 Jan 15;: Authors: Mathuthu E, Janse van Rensburg A, Du Plessis D, Mason S Abstract Biologically important ions (Ca, K, Mg, Fe, and Zn) play major roles in numerous biological processes, with their homeostatic balance being necessary for the maintenance of cellular mechanisms. Sudden and severe loss in homeostasis of just one biologically important ion can cause cascading negative effects. Being able to quickly, accurately and reliably quantify biologically important ions in human bio-fluid samples is something that has been sorely lacking within the field of metabolomics. 1H-NMR is a well-known analytical platform in metabolomics but ions are invisible on 1H-NMR spectra. The foundation of our investigation was based upon a priori knowledge that free EDTA produce two clear single peaks on 1H-NMR spectra, and that EDTA chelated to different ions produce unique 1H-NMR spectral patterns due to 3D conformational changes in the chemical structure of chelated-EDTA and varying degrees of electronegativity. The aim of this study was to develop and test a 1H-NMR-based method, with application specifically to the field of metabolomics, to quantify biologically important ions within the physiological pH range of 6.50-7.50 using EDTA as a chelating agent. Our method produced linear, accurate, precise and repeatable results for Ca, Mg and Zn; h...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research