Making, Cloning and Expression of Human Insulin Genes in Bacteria: The Path to Humulin@.

Making, Cloning and Expression of Human Insulin Genes in Bacteria: The Path to Humulin@. Endocr Rev. 2020 Dec 19;: Authors: Riggs AD Abstract In the mid to late 1970s recombinant DNA methods for cloning and expressing genes in E. coli were under intense development. An important question had become: Can humans design and chemically synthesize novel genes that function in bacteria? This question was answered in 1978 and 1979 with the successful expression in E. coli of two mammalian hormones, first somatostatin and then human insulin. The successful production of human insulin in bacteria provided, for the first time, a practical, scalable source of human insulin and resulted in the approval, in 1982, of human insulin for the treatment of diabetics. In this short review, I give my personal view of how the making, cloning and expressing of human insulin genes was accomplished by a team of scientists led by Keiichi Itakura, Herbert W. Boyer and myself. PMID: 33340315 [PubMed - as supplied by publisher]
Source: ENDOCR REV - Category: Endocrinology Authors: Tags: Endocr Rev Source Type: research