Ngari virus (Orthobunyavirus, Peribunyaviridae) in ixodid ticks collected from cattle in Guinea.

Ngari virus (Orthobunyavirus, Peribunyaviridae) in ixodid ticks collected from cattle in Guinea. Acta Trop. 2020 Dec 09;:105790 Authors: Makenov MT, Toure AH, Bayandin RB, Gladysheva AV, Shipovalov AV, Boumbaly S, Sacko N, Korneev MG, Yakovlev SA, Zhurenkova OB, Grigoreva YE, Fyodorova MV, Radyuk EV, Morozkin ES, Boiro MY, Matsvay A, Khafizov K, Karan LS Abstract Ngari virus is a mosquito-borne virus belonging to the genus Orthobunyavirus (Peribunyaviridae family). This virus is pathogenic to humans and causes severe illness. Ngari virus is present in several African countries, including Madagascar. Here, we report the detection of Ngari virus in ixodid ticks collected from cows in Guinea. A tick survey was conducted in March-November of 2018 in six regions of Guinea. The sample comprised 710 pools, with a total of 2067 ticks belonging to five species collected from 197 cows. At the initial stage, we screened a subsample of tick pools of vector-borne viruses with a multiplex genus-specific primer panel. In the second stage of the study, we narrowed the search and screened all the samples by qPCR for the detection of Ngari virus. All positive samples were sequenced with primers flanking Ngari virus-specific fragments on the S and M segments. We found Ngari virus in 12 pools that were formed from engorged ticks collected from livestock in three villages of the Kindia and Kankan regions. Sequencing of the S and M segments confirmed that...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research