Systems analysis of hematopoiesis using single-cell lineage tracing

Purpose of review In the last few decades, revolutionary advances in next-generation sequencing have led to single-cell lineage tracing technologies that now enable researchers to identify and quantify hematopoietic cell behavior with unprecedented detail. Combined readouts of cell lineage and cell state from the same cell mitigate the need to prospectively isolate populations of interest, and allow a system-level understanding of dynamic developmental processes. We will discuss the advantages and shortcomings of these technologies, the intriguing discoveries that stemmed from lineage tracing hematopoiesis at the single-cell level and the directions toward which the field is moving. Recent findings Single-cell lineage tracing studies unveiled extensive functional heterogeneity within discrete immunophenotypic populations. Recently, several groups merged lineage tracing with single-cell RNA sequencing to visualize clonal relationships directly on transcriptional landscapes without the requirement for prospective isolation of cell types by FACS. To study the cell dynamics of hematopoiesis, without perturbation in their native niche, researchers have developed mouse models with endogenous single-cell lineage tracing systems, which can simultaneously trace thousands of hematopoietic progenitor cells in a single mouse, without transplantation. The emerging picture is that multiple hematopoietic hierarchies coexist within a single individual, each with distinct regulator...
Source: Current Opinion in Hematology - Category: Hematology Tags: MYELOID BIOLOGY: Edited by H. Leighton Grimes Source Type: research

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Publication date: Available online 8 October 2020Source: Hematology/Oncology and Stem Cell TherapyAuthor(s): Ram Vasudevan Nampoothiri, Shiyi Chen, Ivan Pasic, Zeyad Al-Shaibani, Wilson Lam, Fotios V. Michelis, Dennis Dong Hwan Kim, Auro Viswabandya, Armin Gerbitz, Jeffrey Howard Lipton, Rajat Kumar, Jonas Mattsson, Arjun Datt Law
Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
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Source: Supportive Care in Cancer - Category: Cancer & Oncology Source Type: research
Publication date: December 2020Source: Journal of Chemical Neuroanatomy, Volume 110Author(s): Maryam Sadat Jalali, Alireza Sarkaki, Yaghoub Farbood, Seyed saeed Azandeh, Esrafil Mansouri, Mohammad Ghasemi Dehcheshmeh, Ghasem Saki
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research
We report a rare case of HBV reactivation in a patient with HCV infection after DAA therapy. Patient concerns: In 1996, a 53-year-old female was identified as infected with HCV at a medical check-up, following which she visited our hospital. She was infected with HCV genotype 2b, and at follow up in 1997, was found to be hepatitis B surface antigen (HBsAg) and antibody against HBsAg negative, antibody against HBV core positive. She then experienced malignant lymphoma in 2001 at 58 years of age. Complete remission was achieved following chemotherapy and autologous peripheral blood stem cell transplantation. In 2014, she ...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Clinical Case Report Source Type: research
BONE MARROW TRANSPLANTATION (BMT) also called haematopoietic stem cell transplant (HSCT) is the treatment of choice for many haematological diseases including haematological malignancies and different types of anaemia1. The number of HSCT is increasing every year, mainly because of advanced research that has allowed an expansion in the number of indications for which it has become the first-line therapy2. The long-term survival of patients that have undergone BMT continues to improve with several advances in transplantation techniques and supportive care practices.
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
Chronic graft-versus-host disease (cGvHD) is a severe complication of allogeneic hematopoietic stem cell transplantation that affects various organs leading to a reduced quality of life. The condition often requires enduring immunosuppressive therapy, which can also lead to the development of severe side effects. Several approaches including small molecule inhibitors, antibodies, cytokines, and cellular therapies are now being developed for the treatment of cGvHD, and some of these therapies have been or are currently tested in clinical trials. In this review, we discuss these emerging therapies with particular emphasis on...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Epstein –Barr virus (EBV) is associated with the pathogenesis of a variety of malignancies, most notably lymphomas. Especially in the background of immunodeficiency, such as primary immunodeficiency disorder (P...
Source: Diagnostic Pathology - Category: Pathology Authors: Tags: Case Report Source Type: research
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Source: International Orthopaedics - Category: Orthopaedics Source Type: research
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Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Condition:   Hematopoietic Stem Cell Transplantation Intervention:   Procedure: Hematopoietic stem cell transplantation Sponsor:   IRCCS Burlo Garofolo Completed
Source: - Category: Research Source Type: clinical trials
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