Molecules, Vol. 25, Pages 5192: Antiglioma Potential of Coumarins Combined with Sorafenib

Molecules, Vol. 25, Pages 5192: Antiglioma Potential of Coumarins Combined with Sorafenib Molecules doi: 10.3390/molecules25215192 Authors: Joanna Sumorek-Wiadro Adrian Zając Ewa Langner Krystyna Skalicka-Woźniak Aleksandra Maciejczyk Wojciech Rzeski Joanna Jakubowicz-Gil Coumarins, which occur naturally in the plant kingdom, are diverse class of secondary metabolites. With their antiproliferative, chemopreventive and antiangiogenetic properties, they can be used in the treatment of cancer. Their therapeutic potential depends on the type and location of the attachment of substituents to the ring. Therefore, the aim of our study was to investigate the effect of simple coumarins (osthole, umbelliferone, esculin, and 4-hydroxycoumarin) combined with sorafenib (specific inhibitor of Raf (Rapidly Accelerated Fibrosarcoma) kinase) in programmed death induction in human glioblastoma multiforme (T98G) and anaplastic astrocytoma (MOGGCCM) cells lines. Osthole and umbelliferone were isolated from fruits: Mutellina purpurea L. and Heracleum leskowii L., respectively, while esculin and 4-hydroxycoumarin were purchased from Sigma Aldrich (St. Louis, MO, USA). Apoptosis, autophagy and necrosis were identified microscopically after straining with specific fluorochromes. The level of caspase 3, Beclin 1, PI3K (Phosphoinositide 3-kinase), and Raf kinases were estimated by immunoblotting. Transfection with specific siRNA (small interfering RNA) was used to block Bcl-2 (B-...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research