NaCl concentration-dependent aminoglycoside resistance of Halomonas socia CKY01 and identification of related genes.

NaCl concentration-dependent aminoglycoside resistance of Halomonas socia CKY01 and identification of related genes. J Microbiol Biotechnol. 2020 Nov 04;: Authors: Park YL, Choi TR, Kim HJ, Song HS, Lee HS, Park SL, Lee SM, Kim SH, Park S, Bhatia SK, Gurav R, Sung C, Seo SO, Yang YH Abstract Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, there was not much information on antibiotic resistance of Halomonas species. As an effort to find antibiotic resistance of Halomonas socia CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics, including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the H. socia CKY01 resistance pattern to kanamycin, gentamicin, apramycin, tetracycline, and spectinomycin was different in the presence of 10% NaCl and 1% NaCl in the culture medium. To determine the mechanism underlying NaCl concentration-dependent antibiotic resistance, we compared four aminoglycoside resistance genes under different salt conditions, and by performing time dependent reverse transcription PCR. We found that aminoglycoside phosphotransferase (aph2) showed increased expression under the 10% than 1% NaCl conditions. When these genes were overexpressed in ...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research