IJERPH, Vol. 17, Pages 7827: Hospital Wastewater —Important Source of Multidrug Resistant Coliform Bacteria with ESBL-Production

IJERPH, Vol. 17, Pages 7827: Hospital Wastewater—Important Source of Multidrug Resistant Coliform Bacteria with ESBL-Production International Journal of Environmental Research and Public Health doi: 10.3390/ijerph17217827 Authors: Kristína Lépesová Petra Olejníková Tomáš Mackuľak Klára Cverenkárová Monika Krahulcová Lucia Bírošová This work compares the prevalence of antibiotic resistant coliform bacteria in hospital wastewater effluents in Slovak (SR) and Czech Republic (ČR). It also describes selected antibiotic resistant isolates in view of resistance mechanism and virulence factor. The highest number of multidrug resistant bacteria was detected in samples from the hospital in Valašské Meziříčí (ČR). More than half of resistant isolates showed multidrug resistance phenotype as well as strong ability to form biofilm. In 42% of isolates efflux pump overproduction was detected together with tetA and tetE genes. The production of extended-spectrum β-lactamases in coliform isolates was encoded mainly by blaTEM, blaCTX-M-2 and blaCTX-M-8/25 genes. About 62% of resistants contained a combination of two or more extended spectrum beta-lactamases (ESBL) genes. Our results strengthen the fact that hospital effluents are a source of multidrug resistant bacteria which can spread their resistance genes to other bacteria in wastewater treatment plants (WWTPs). Accordingly, hosp...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research