An update and perspectives on the use of promoters in plant genetic engineering.

An update and perspectives on the use of promoters in plant genetic engineering. J Biosci. 2020;45: Authors: Kummari D, Palakolanu SR, Kishor PBK, Bhatnagar-Mathur P, Singam P, Vadez V, Sharma KK Abstract Genetically engineered plants have varied applications in agriculture for enhancing the values of food and feed. Genetic engineering aims to introduce selected genetic regions with desirable traits into target plants for both spatial and temporal expressions. Promoters are the key elements responsible for regulating gene expressions by modulating the transcription factors (TFs) through recognition of RNA polymerases. Based on their recognition and expression, RNA polymerases were categorized into RNA pol II and pol III promoters. Promoter activity and specificity are the two prime parameters in regulating the transgene expression. Since the use of constitutive promoters like Cauliflower mosaic virus (CaMV) 35S may lead to adverse effects on nontarget organisms or ecosystem, inducible/tissue specific promoters and/or the RNA pol III promoters provide myriad opportunities for gene expressions with controlled regulation and with minimum adverse effects. Besides their role in transgene expression, their influence in synthetic biology and genome editing are also discussed. This review provides an update on the importance, current prospects, and insight into the advantages and disadvantages of promoters reported thus far would help to uti...
Source: Journal of Biosciences - Category: Biomedical Science Authors: Tags: J Biosci Source Type: research