Octadecyl 3-(3, 5-di-tert-butyl-4-hydroxyphenyl) propanoate, an antifungal metabolite of Alcaligenes faecalis strain MT332429 optimized through response surface methodology.

Octadecyl 3-(3, 5-di-tert-butyl-4-hydroxyphenyl) propanoate, an antifungal metabolite of Alcaligenes faecalis strain MT332429 optimized through response surface methodology. Appl Microbiol Biotechnol. 2020 Oct 22;: Authors: El-Sayed SE, Abdelaziz NA, El-Housseiny GS, Aboshanab KM Abstract In the current study, a soil bacterial isolate F2 expressed a significant antagonistic activity against Candida albicans ATCC 10231 and Aspergillus niger clinical isolate confirmed through cross streak, dual culture, and agar well diffusion methods. The isolate F2 was identified using phenotypic and molecular approaches as Alcaligenes (A.) faecalis MT332429. The identification and structural characterization of the antifungal compound was performed using advanced spectroscopic techniques including UV absorbance, 1H and 13C NMR and 2D NMR (COSY, HSQC, and HMBC) and was identified as octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoate. Response surface methodology (RSM) using a central composite design was employed to optimize the nutritional and cultural variables affecting the antifungal metabolite yield. The optimum conditions were found to be temperature 30 °C, agitation 150 rpm, glucose 1 g/l, peptone 2 g/l, and pH 8. A confirmatory experiment was performed to assess the accuracy of the optimization procedure, where an increase in the antifungal metabolite production by about 2.48-fold was obtained. To the best of our knowledge, this is t...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research