In Vitro Antiosteoporosis Activity and Hepatotoxicity Evaluation in Zebrafish Larvae of Bark Extracts of Prunus jamasakura Medicinal Plant.

In this study, in vitro antiosteoporosis activity and hepatotoxicity of P. jamasakura bark extracts were evaluated. Methods. Mouse bone marrow macrophage (BMM) cells were incubated with tartrate-resistant acid phosphate (TRAP) buffers and p-nitrophenyl phosphate and cultured with different P. jamasakura bark extracts at concentrations of 0, 6.25, 12.5, 25, and 50 μg/ml in the presence of the receptor activator of nuclear factor kappa-Β ligand (RANKL) for 6 days. The osteoclast TRAP activity and cell viability were measured. Nitric oxide (NO) assay was conducted using murine macrophage-like RAW 264.7 cells treated with P. jamasakura ethanolic and methanolic bark extracts at concentrations of 0, 6.25, 12.5, 25, 50, 100, and 200 μg/ml. For hepatotoxicity assessment, zebrafish larvae were exposed to P. jamasakura bark extracts, 0.05% dimethyl sulfoxide as a negative control, and 5 μM tamoxifen as a positive control. The surviving larvae were anesthetized and assessed for hepatocyte apoptosis. Results. TRAP activity was significantly inhibited (p 
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research

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Authors: Liu X, Zheng J, Li F, Yi R, Mu J, Tan F, Zhao X Abstract A rat model of secondary osteoporosis was constructed using retinoic acid as an inducer, and the genes, proteins, and bone mass of the rats were analyzed. qPCR detection of the Wnt/β-catenin and OPG/RANK/RANKL signaling pathway-related gene expression levels showed that Lactobacillus plantarum HFY15 played a positive role in regulating both pathways. HFY15 significantly increased β-catenin, Lrp5, Lrp6, Wnt10b, OPG, RANKL, and Runx2 expression and downregulated DKK1, RANK, CTSK, TRACP, and ALP expression. Enzyme-linked immunosorbent assays f...
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Publication date: December 2020Source: Journal of Functional Foods, Volume 75Author(s): Haiying Liu, Lu Huang, Jing Wang
Source: Journal of Functional Foods - Category: Nutrition Source Type: research
ConclusionThis is the first population-based study to ascertain the incidence of major non-vertebral fractures in elderly Latin Americans, confirming the high frequency of the disorder. Age, prior fracture, and hip BMD were predictors of the short-term incidence of fracture.
Source: Osteoporosis International - Category: Orthopaedics Source Type: research
AbstractPurpose of ReviewThis paper seeks to review the important musculoskeletal issues that arise for pregnant and postpartum women. It outlines the background, diagnosis, and management of these musculoskeletal issues; reviews the existing and recent literature; and offers clinical opinions from the perspective of women ’s health PM&R physicians.Recent FindingsExisting and recent literature on pelvic girdle pain, lumbosacral back pain, pubic symphysis separation, transient osteoporosis, rectus diastasis, and postpartum neuropathy offer some new insights on management, which is often context-dependent. Management r...
Source: Current Physical Medicine and Rehabilitation Reports - Category: Rehabilitation Source Type: research
AbstractPurpose of Review3D cone beam imaging (CBCT) has allowed clinicians to better understand the anatomical variations of cranial anatomy. One crucial aspect of this technology plays is the understanding of alveolar bone morphology and remodeling. Variations in cortical bone thickness between individuals have been reported. No published study has analyzed the relationship between cortical bone thickness and rate of tooth movement. The aim of this study is to begin answering the question: is there an association between rate of tooth movement and cortical bone thickness?Recent FindingsTwenty-three patients underwent ext...
Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
Serum anti-Müllerian hormone levels are associated with low bone mineral density in premenopausal women. Biomarkers. 2020 Oct 07;:1-23 Authors: Yan Y, Chen W, Wang J, Huang J, Lv J, Zhao H, Guo L Abstract Objective: To investigate the associations between anti-Müllerian hormone (AMH) and bone mineral density (BMD) induced by ovarian insufficiency in premenopausal women.Methods: Subjects were consecutively enrolled from January 2015 to December 2018. Dual energy X-ray absorptiometry examination was set as the gold standard, with T-scores less than -2.5/-1 as thresholds for the definition of os...
Source: Biomarkers - Category: Research Tags: Biomarkers Source Type: research
The objective of this study was to analyse hospital incidence of osteogenesis imperfecta (OI) in Spanish hospitals and the associated medical costs from a healthcare system perspective.Methods: To this aim, a retrospective multicentre study was designed analysing admission records from patients admitted with OI in specialised care settings in Spain between 2000 and 2017. Direct medical costs were calculated based on the diagnosis-related group-based hospital payment systems, determined by the Spanish Ministry of Health.Results: Overall, 3,747 admissions were reviewed, corresponding to 998 patients, 48.20% of which were mal...
Source: Journal of Medical Economics - Category: Health Management Tags: J Med Econ Source Type: research
This study aims to determine the association between MetS and its components and bone mineral density (BMD) assessed by dual-energy X-ray absorptiometry (DXA) among Malaysians. Methods: 400 Malaysians aged ≥ 40 years (52.5% women) residing in Klang Valley, Malaysia, were recruited. Subjects' demographic and lifestyle details were collected using a questionnaire, and blood pressure and body anthropometry were measured. Subjects' lumbar spine and total hip BMD were measured by DXA. Their fasting blood was collected for blood glucose level and lipid profile analysis. Regression analysis was used to analyze the relationship...
Source: International Journal of Medical Sciences - Category: Biomedical Science Tags: Int J Med Sci Source Type: research
Comorbidity and health care utilisation in persons with Sjögren's syndrome: a claims data analysis. Clin Exp Rheumatol. 2020 Sep 18; Authors: Albrecht K, Dörner T, Redeker I, Karberg K, Marschall U, Zink A, Callhoff J Abstract OBJECTIVES: To capture comorbidity and medication of persons with Sjögren's syndrome (SS) in a population-based cohort in comparison to matched controls. METHODS: Individuals with an outpatient diagnosis of M35.0 (ICD-10) in ≥2 quarters of a year or an inpatient diagnosis of M35.0 were identified in a German statutory health insurance fund covering 7.2 milli...
Source: Clinical and Experimental Rheumatology - Category: Rheumatology Tags: Clin Exp Rheumatol Source Type: research
Publication date: Available online 28 July 2020Source: International Journal of Orthopaedic and Trauma NursingAuthor(s): D.S. Anupama, Judith A. Norohna, Kiran KV. Acharya, Ravishankar, Anice George
Source: International Journal of Orthopaedic and Trauma Nursing - Category: Nursing Source Type: research
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