GSE147629 Development of an in vivo ligated loop model reveals new insight into the host immune response against  Campylobacter jejuni

Contributors : Nicholas M Negretti ; Yinyin Ye ; Lais M Malavasi ; Swechha M Pokharel ; Steven Huynh ; Susan Noh ; Christopher R Gourley ; Claude A Ragle ; Santanu Bose ; Torey Looft ; Craig T Parker ; Geremy Clair ; Joshua N Adkins ; Michael E KonkelSeries Type : Expression profiling by high throughput sequencingOrganism : Campylobacter jejuniThe symptoms of infectious diarrheal disease are mediated by the interplay between the host and pathogen. Campylobacter jejuni is the leading bacterial cause of diarrhea worldwide due to its near-ubiquitous zoonotic association with poultry. One of the outstanding questions is what factors drive the intestinal inflammation during the development of C. jejuni-mediated disease. Specifically, it is not known the extent to which the bacteria are responsible for the diarrheal symptoms via cell necrosis, or whether there is immune cell recruitment prior to tissue damage. To determine the stepwise process of inflammation that leads to diarrhea, we used a piglet ligated intestinal loop model to study the intestinal environment in response to C. jejuni. Pigs were chosen due to the anatomical similarity of the porcine intestine to the human intestine, as the basis of disease modeling is to understand the process of human disease. Using immunoassays and proteomic approaches, we found that neutrophils are most likely the predominant cell type recruited to the intestines of C. jejuni infected animals. In the lumen of the intestine, a number of...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Campylobacter jejuni Source Type: research