Cancers, Vol. 12, Pages 2731: Molecular Pathogenesis of Pancreatic Ductal Adenocarcinoma: Impact of miR-30c-5p and miR-30c-2-3p Regulation on Oncogenic Genes
Cancers, Vol. 12, Pages 2731: Molecular Pathogenesis of Pancreatic Ductal Adenocarcinoma: Impact of miR-30c-5p and miR-30c-2-3p Regulation on Oncogenic Genes
Cancers doi: 10.3390/cancers12102731
Authors:
Takako Tanaka
Reona Okada
Yuto Hozaka
Masumi Wada
Shogo Moriya
Souichi Satake
Tetsuya Idichi
Hiroshi Kurahara
Takao Ohtsuka
Naohiko Seki
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive types of cancer, and its prognosis is abysmal; only 25% of patients survive one year, and 5% live for five years. MicroRNA (miRNA) signature analysis of PDAC revealed that both strands of pre-miR-30c (miR-30c-5p, guide strand; miR-30c-2-3p, passenger strand) were significantly downregulated, suggesting they function as tumor-suppressors in PDAC cells. Ectopic expression assays demonstrated that these miRNAs attenuated the aggressiveness of PDAC cells, e.g., cell proliferation, migration, and invasiveness. Through a combination of in silico analyses and gene expression data, we identified 216 genes as putative oncogenic targets of miR-30c-5p and miR-30c-2-3p regulation in PDAC cells. Among these, the expression of 18 genes significantly predicted the 5-year survival rates of PDAC patients (p < 0.01). Importantly, the expression levels of 10 genes (YWHAZ, F3, TMOD3, NFE2L3, ENDOD1, ITGA3, RRAS, PRSS23, TOP2A, and LRRFIP1) were found to be independent prognostic factors for patient survival (p < 0.01). We focused on TOP2A (DNA T...
Source: Cancers - Category: Cancer & Oncology Authors: Takako Tanaka Reona Okada Yuto Hozaka Masumi Wada Shogo Moriya Souichi Satake Tetsuya Idichi Hiroshi Kurahara Takao Ohtsuka Naohiko Seki Tags: Article Source Type: research