Clinical significance of periodic detection of HBV YVDD mutation by ultrasensitive RT-ARMS-qPCR during lamivudine treatment in patients with chronic hepatitis B.

Clinical significance of periodic detection of HBV YVDD mutation by ultrasensitive RT-ARMS-qPCR during lamivudine treatment in patients with chronic hepatitis B.
J Med Microbiol. 2015 Jan 16;
Authors: Zeng Y, Yang B, Wu Y, Chen J, Shang H, Chen X, Su M, Wu S, Lin J, Ou Q
Abstract
Monitoring HBV mutants periodically during nucleoside analogues treatment is of great clinical significance, particularly in patients with persistent HBV DNA-positive. However, few studies have investigated the dynamic changes of HBV YMDD (tyrosine-methionine-aspartate-aspartate) and YVDD (tyrosine-valine-aspartate-aspartate) populations in chronic hepatitis B patients while undergoing lamivudine treatment. We sought to investigate the dynamic changes of HBV YMDD and HBV YVDD variants by ultrasensitive real-time amplification refractory mutation system quantitative PCR (RT-ARMS-qPCR) and evaluate its significance for the changes in treatment in CHB patients. RT-ARMS-qPCR was established and evaluated with standard recombinant plasmids. 15 CHB patients receiving lamivudine (100 mg daily) were consecutively recruited and followed up for 60 weeks. Serum samples were obtained from each patient at baseline and every 12 weeks. The total HBV DNA, HBV YMDD DNA and YVDD DNA levels were measured using RT-ARMS-qPCR at all given time points after treatment. Routine liver biochemistry parameters including AST and ALT were also measured. The linear range of the assay was ...

Source: Journal of Medical Microbiology - January 16, 2015 Category: Microbiology Authors: Zeng Y, Yang B, Wu Y, Chen J, Shang H, Chen X, Su M, Wu S, Lin J, Ou Q Tags: J Med Microbiol Source Type: research